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Birsa Agricultural University, Ranchi
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ThesisItem Open Access Bacteriological Quality of Pork and pork product with special reference to Enterotoxigenic(Birsa Agricultural University, Ranchi, 1992) Mohammad Kalimuddin; S.P.ChoudharyIt was also concluded that while studying enteropathogenicity/enterotoxigenicity of the isolates of Salmonella, consideration of the results of histopathological changes in the Intestinal loops of animal model is also essential atleast for those loops showing dilstation index less than 0.40.ThesisItem Open Access Bacteriological Quality of Pork Luncheon Meat with Special Refrence to Escherichia Coli(Birsa Agricultural University, Ranchi, 1994) Nelli Grass Toppo; Dr. K. G. NarayanAll the serotypes of E. coli were enter pathogenic. Serotypes 0117 and 0147 were found to be more enter pathogenic than other serotypes of E. coll. This was further confirmed with histopathological changes observed in the sections of intestinal loops. Both the serotypes 0117 and 0147 showed extensive loss of intestinal villi, leaving behind a few villi, the height of which were greatly reduced. They also caused wide spread of hemorrhage in subserosal area and lamina proprias. Serotypes 06, 07, 065, 0117 and 0147 were also enter toxigenic as revealed by rabbit 11eal loop and suckling infant mice tests and finally confirmed with histopathological changes in the intestinal loops. It was concluded that while studying enter pathogenicity/ enterotaxigenicity of the isolates of E. coli. Consideration of the results of histopathological changes in the intestinal loops of animal model is also essential at least for those loops showing dilatation index less than 0.40. The presence of enter pathogenic/ enter toxigenic serotypes of coli in pork luncheon meat is alarming From public health point of view, it is important that measures must be taken to prevent contamination of this "ready-to-eat" pork product during its manufacture at different states of production.ThesisItem Open Access Biotyping and Serotyping of Bacillus Cereus Isolated from fried Rice and Chawmin(Birsa Agricultural University, Ranchi, 1993) Rajendra Yadav; K.G.NarayanBacillus cercus has recently emerged as one of the most important food borne pathogen in India. It is most commonly associated with cereal foods causing diarrhea and vomition in human being. As compared to other food borne pathogens relatively little work has been done on . cercus particularly with fast foods. Therefore, the present investigation was undertaken to see the incidence of this organism in chawmin and fried rice. Skimmed milk powder was also included in this study. Altogether 226 samples consisting of chowmin (100), fried rice (100) and skimmed milk powder (26) were examined. The result has been presented in log10 scale. Altogether 23.0, 24.0 and 69.23 per cent of the samples of chaumin, fried rice and skimmed milk powder were positive for cereus and the counts ranged between zero to 3.9193/0, zero to 3.6182/g and zero to 3.6812/g with an average of 0.6453 0.1225, 0.7394 0.1400 and 1.9386 0.2736/s, respectively. Mannitol egg yolk polymixin agar appears to be a satisfactory selective and differential medium for cereus which is inhibitory to many bacteria and only a limited group of organisms grew on it. Viz. S. aureus, Serratia marcescens and P. vulgaris. The number of such bacteria was also estimated and designated as bacteria other than cercus. Altogether 74.0, 64.0 and zero per cent of the samples of choumin, fried rice and skimmed milk powder were positive for bacteria other than cercus and the count ranged from zero to 5.4771, zeroThesisItem Open Access A Study on the Enteropathy of Salmonellae and Their Enterotoxins(Birsa Agricultural University, Ranchi, 1993) Bhaskar Debnath; S.P.ChoudharyAll the serotypes of Salmonella showed vascular permeability reactions (VPR) on rabbit skin. Therefore, it was concluded that VPR test might be employed as an alternative assay system for screening enterotoxigenic Salmonelise. There was variation in the heat stability of the enterotoxins of S ohlo, S. bornum and Salmonella (3:10:r:-). While the CFS of S. ohfo induced enough fluid accumulation in RLIL to consider it positive for enterotoxic activity after heating at 70°c for 30 minutes the CPS of S bornum and Salmonella (3:10:r:-) showed decrease in the enterotoxicity activity at 70°C for 30 minutes. However, the CFS of all the serotypes of Salmonella were found to be heat labile and lost their enterotoxicity at 80°C for 30 minutes. The enterotoxic activity of the CFS of all the Salmonellae maintained between pH 6.0 and 8.0. The enterotoxins were sensitive to proteolytic enzyme trypsin. The enterotoxins present in CFS of the organisms were salt precipitable with ammonium sulphate at 60.0 per cent saturation level. It was concluded that enteropathy of diarrhoea associated with Sohio, S. bornum and Salmonella (3:10:r:-) was enterotoxin mediated.ThesisItem Open Access Rabies Infection and protection in Pubs less than 10- 12 weeks(Birsa Agricultural University, Ranchi, 1991) Anjan Bhattacharya; K.G.NarayanNinety nine apparently healthy street pups of 10-12 weeks of age were found to be free from rabies virus. This is based on simultaneous examination of brain, salivary gland and tonsil of each of 99 pups. The results of examining the above 297 samples simultaneously by 5 different tests vis., MIT, FAT, IPT, DOT-ELISA and Seller's stain add strength in declaring that the 99 pups examined were negative for rabies. Serologic tests like FAT and IPT were comparable to MIT as was earlier reported by Department of Veterinary Public Health & Epidemiology. In addition ELISA and DOT-ELISA tests were carried out. The former, reported from Pasteur Institute, Paris was found to be equally comparable. DOT-ELISA was developed in order to make rabies diagnosis easy. Results of ELISA and DOT-ELISA compared with MIT suggested that it was possible to titrate rabies virus antigen. Serum neutralization test in mice carried out in this study was comparable to those being routinely done at Central Research Institute, Kasauli. The three testa, viz., SN, ELISA and DOT-ELISA were used to monitor the response of vaccination of pups 4 weeks old with Raksharab and Rabisin. The of the three tests were comparable thus indicating that one could determine anti rabic serum titre by ELISA and DOT-ELISA instead of SN.ThesisItem Open Access STANDARDIZATION OF IMMUNOPEROXIDASE TESTS FOR THE DIAGNOSIS OF RABIES(Birsa Agricultural University, 1984) KOTWAL, SANJAY; Narayanan, K.G.The present study was conducted to standardize tests which are comparable in specificity to Fluorescent antibody and Mouse inoculation tests for the diagnosis of rabies. A total of 95 specimens, consisting of 53 infected and 42 normal brain tissue were tested. The infected specimens included 11 CVS-infected mice brains, eight specimens from field cases and 34 brains from the mice infected with the field material. The 42 normal brain originate from mouse. Immunoperoxidase tests vis., Direct IPT, Indirect IPT and Peroxidase-Anti-Peroxidase (PAP) alongwith Indirect Fluorescent Antibody Test (PAT) were standardized for the specific diagnosis of rabies. Simultaneously, mouse inoculation, Direct FAT and Seller's staining were carried out. While each of these 4 tests gave 100.0 percent specific reaction, the sensitivity differed. The sensitivity of Direct IPT was 95.3 percent, compared to 98.0 percent of Indirect IPT and 100.0 percent of each of PAP and Indirect FAT. The immunoperoxidase tests perform well on the formalin preserved and paraffin embedded tissues and it was easy to recognize the reaction on the histopathological sections. Histopathological sections were also stained by Seller's and Haematoxylere and Eosin stain to demonstrate Negri bodies. The study was extended to see whether the acetone fixed smears stored at room temperature could be stained specifically for the viral antigen. The viral antigen could be detected only up to 4 days.
