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Birsa Agricultural University, Ranchi

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1993 - 19943
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Subject: Veterinary Epidemiology and Preventive Medicine × End date: 1997 × Start date: 1993 × Type: Thesis ×
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    ThesisItemOpen Access
    Bacteriological Quality of Pork Luncheon Meat with Special Refrence to Escherichia Coli
    (Birsa Agricultural University, Ranchi, 1994) Nelli Grass Toppo; Dr. K. G. Narayan
    All the serotypes of E. coli were enter pathogenic. Serotypes 0117 and 0147 were found to be more enter pathogenic than other serotypes of E. coll. This was further confirmed with histopathological changes observed in the sections of intestinal loops. Both the serotypes 0117 and 0147 showed extensive loss of intestinal villi, leaving behind a few villi, the height of which were greatly reduced. They also caused wide spread of hemorrhage in subserosal area and lamina proprias. Serotypes 06, 07, 065, 0117 and 0147 were also enter toxigenic as revealed by rabbit 11eal loop and suckling infant mice tests and finally confirmed with histopathological changes in the intestinal loops. It was concluded that while studying enter pathogenicity/ enterotaxigenicity of the isolates of E. coli. Consideration of the results of histopathological changes in the intestinal loops of animal model is also essential at least for those loops showing dilatation index less than 0.40. The presence of enter pathogenic/ enter toxigenic serotypes of coli in pork luncheon meat is alarming From public health point of view, it is important that measures must be taken to prevent contamination of this "ready-to-eat" pork product during its manufacture at different states of production.
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    ThesisItemOpen Access
    Biotyping and Serotyping of Bacillus Cereus Isolated from fried Rice and Chawmin
    (Birsa Agricultural University, Ranchi, 1993) Rajendra Yadav; K.G.Narayan
    Bacillus cercus has recently emerged as one of the most important food borne pathogen in India. It is most commonly associated with cereal foods causing diarrhea and vomition in human being. As compared to other food borne pathogens relatively little work has been done on . cercus particularly with fast foods. Therefore, the present investigation was undertaken to see the incidence of this organism in chawmin and fried rice. Skimmed milk powder was also included in this study. Altogether 226 samples consisting of chowmin (100), fried rice (100) and skimmed milk powder (26) were examined. The result has been presented in log10 scale. Altogether 23.0, 24.0 and 69.23 per cent of the samples of chaumin, fried rice and skimmed milk powder were positive for cereus and the counts ranged between zero to 3.9193/0, zero to 3.6182/g and zero to 3.6812/g with an average of 0.6453 0.1225, 0.7394 0.1400 and 1.9386 0.2736/s, respectively. Mannitol egg yolk polymixin agar appears to be a satisfactory selective and differential medium for cereus which is inhibitory to many bacteria and only a limited group of organisms grew on it. Viz. S. aureus, Serratia marcescens and P. vulgaris. The number of such bacteria was also estimated and designated as bacteria other than cercus. Altogether 74.0, 64.0 and zero per cent of the samples of choumin, fried rice and skimmed milk powder were positive for bacteria other than cercus and the count ranged from zero to 5.4771, zero
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    ThesisItemOpen Access
    A Study on the Enteropathy of Salmonellae and Their Enterotoxins
    (Birsa Agricultural University, Ranchi, 1993) Bhaskar Debnath; S.P.Choudhary
    All the serotypes of Salmonella showed vascular permeability reactions (VPR) on rabbit skin. Therefore, it was concluded that VPR test might be employed as an alternative assay system for screening enterotoxigenic Salmonelise. There was variation in the heat stability of the enterotoxins of S ohlo, S. bornum and Salmonella (3:10:r:-). While the CFS of S. ohfo induced enough fluid accumulation in RLIL to consider it positive for enterotoxic activity after heating at 70°c for 30 minutes the CPS of S bornum and Salmonella (3:10:r:-) showed decrease in the enterotoxicity activity at 70°C for 30 minutes. However, the CFS of all the serotypes of Salmonella were found to be heat labile and lost their enterotoxicity at 80°C for 30 minutes. The enterotoxic activity of the CFS of all the Salmonellae maintained between pH 6.0 and 8.0. The enterotoxins were sensitive to proteolytic enzyme trypsin. The enterotoxins present in CFS of the organisms were salt precipitable with ammonium sulphate at 60.0 per cent saturation level. It was concluded that enteropathy of diarrhoea associated with Sohio, S. bornum and Salmonella (3:10:r:-) was enterotoxin mediated.