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Birsa Agricultural University, Ranchi

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2011 - 20155
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Subject: Animal Reproduction and Gynecology × Start date: 2010 × Type: Thesis ×
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    ThesisItemOpen Access
    EFFECT OF PROSTAGLANDIN AND OXYTOCIN ADMINISTRATION ON SEMINAL CHARACTERISTICS IN RAM
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2011) Singh, Amit Ranjan Kr.; Singh, M.P.
    The present study was conducted on six rams of chottanagpuri breed. The rams were divided into two groups, each consisting of three rams. Oxytocin 7 IU to each ram in one group (oxytocin group) and prostaglandin F2α 0.1mg/kg body weight to each ram in other group (prostaglandin group were administered before semen collection. Scrotal circumference in each ram was measured and correlation with seminal attributes was studied. Effect of oxytocin and prostaglandin F2α on seminal attributes were also studied. 1. Average scrotal circumference in rams was found to be 22.33±0.30 and 22.33±0.22 cm in oxytocin and prostaglandin groups respectively. 2. Non-significant positive correlation of scrotal circumference with mass activity and sperm concentration in oxytocin group and nonsignificant positive correlation with ejaculate volume, mass activity, sperm concentration and live sperm percentage in prostaglandin group were observed. 3. Colour of the neat semen was observed to be creamy or creamy white. 4. The overall average ejaculate volume of semen before treatment and after treatment in oxytocin group were 0.52±0.02 ml and 0.72±0.02 ml respectively whereas in prostaglandin group 0.56±0.16 ml and 0.83±0.02 ml respectively. The overall mean values before and after treatment differed significantly in both the groups. 5. Non-significant effect of treatment on seminal pH was observed in both the groups. 6. The overall mean values of mass activity before and after treatment were 3.77±0.10 and 3.99±0.05 respectively in oxytocin group and in prostaglandin group the above values were 3.88±0.77 and 4.00±0.60 respectively, but pre-treatment and post treatment values did not differ significantly in both the groups. Abstract ………   7. The overall mean values of sperm concentration before and after treatment in oxytocin group were 3729±78.86 and 3609±111.02 millions per ml respectively whereas in prostaglandin group the values were 3528±103.44 and 3981±46.20 millions per ml respectively. 8. Pre-treatment and post- treatment values of sperm concentration did not differ significantly in oxytocin group but differed significantly (p≤0.05) in prostaglandin group. 9. With respect to live sperm percentage, the overall mean values before treatment and after treatment were 81.94±0.71 and 82.86±0.53 per cent respectively in oxytocin group whereas the values were 82.33±1.00 and 83.73±0.72 per cent respectively in prostaglandin group. Differences between pre-treatment and post-treatment values were non-significant in both the groups. 10. The overall mean values of abnormal sperm percentage before treatment and after treatment in oxytocin group were 6.40±0.59 and 6.20±0.48 respectively whereas in prostaglandin group the values were 6.94±0.62 and 6.40±0.42 respectively. Differences between pretreatment and post- treatment values were non-significant in both the groups. Out of total sperm abnormalities, sperm tail abnormalities were found to be highest. 11. Significant increase in ejaculate volume, sperm concentration and sperm head abnormalities was recorded after prostaglandin administration. It may be concluded from this study that scrotal circumference was positively correlated with mass activity and sperm concentration in the rams of both the groups. It may also be concluded that both oxytocin and prostaglandin administration increased ejaculate volume of semen significantly. Significant increase in sperm concentration was also recorded with prostaglandin treatment during this study.
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    ThesisItemOpen Access
    COMPARATIVE EFFICACY OF CERTAIN IMMUNOMODULATORS FOR THE TREATMENT OF ENDOMETRITIS IN COWS
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2011) Kant, Ravi; Sinha, M.P.
    The levamisole acted as a good immunomodulator for the treatment of endometritis in cows at the dose rate of 1ml/30 kg body weight administered through sub-cutaneous route three times at an interval of 7 days. Septilin (a herbal preparation) at the dose rate of 100 ml per animal given orally continuously for 15 days was also found effective in controlling endometritis in cows, although levamisole was more effective than septilin. Hence, levamisole may be used for the treatment of endometritis as an alternate therapy to antibiotics.
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    ThesisItemOpen Access
    INFLUENCE OF ANTIOXIDANTS, REDUCED GLUTATHIONE AND VITAMIN E ON MICROSCOPIC AND OXIDATIVE STRESS PARAMETERS OF FROZEN THAWED CHHOTANAGPURI RAM SEMEN
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2014) KURMI, DHRUBA JYOTI; Singh, M. P.
    The result of the present study shows that addition of vitamin E and reduced glutathione in Tris freezing medium improved the percentage of sperm motility, live sperm, plasma membrane integrity, acrosomal integrity and DNA integrity in the frozen thawed semen compared to the Tris control as freezing medium. 2mM Vit E added in Tris dilutor appeared to be the best. Thus on the basis of the present result 2mM Vit E and 5mM GSH may be recommended for supplementation in Tris freezing medium for freezing of Chhotanagpuri ram semen. However further study on large population is required for validation of the result.
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    ThesisItemOpen Access
    “STUDIES ON CRYOPRESERVATION AND POSTTHAWED ASSESMENT OF CHHOTANAGPURI RAM SEMEN”
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2013) Toppo, Tej Pawan; Sinha, M. P.
    The studies were carried out on seminal ejaculate characteristics and its cryopreservation of Chhotanagpuri rams. The main purpose of this study was to determine the cryotolerance of Chhotanagpuri ram spermatozoa on the basis of post-thaw semen quality. The results obtained have been discussed earlier in detail, the same have been summarized as follows: Seminal characters at pre- freeze stage: 1. The colour of Chhotanagpuri ram semen was found to be creamy or creamy white. 2. The overall mean ejaculate volume, mass motility, sperm concentration and live sperm percentage were found to be 0.56±0.02 ml, 4.28±0.08, 4210.42±29.00 millions/ml and 84.50±0.65 percent respectively. 3. The overall mean value of intact plasma membrane integrity, intact acrosome status and intact DNA integrity were found to be 78.11±0.63 percent, 83.42±0.75 percent and 89.97±0.38 percent respectively. 4. Mean live percentage, intact plasma membrane integrity, intact acrosome, and intact DNA integrity percentage were significantly higher in neat semen samples than frozen thawed semen. 5. The ejaculate volume was negatively and non significantly correlated with sperm concentration, acrosome status and DNA integrity .The ejaculate volume was significantly (P<0.05) and positively correlated with mass motility. It was positively but non significantly correlated with live sperm % and plasma membrane integrity. 6. The mass motility was significantly (P<0.01) and positively correlated with sperm concentration, live sperm percentage. Its correlation with acrosome status, plasma membrane integrity and DNA integrity was positive but non significant. 7. The sperm concentration was highly significantly (P<0.01) and positively correlated with live sperm percentage whereas it was positively and non significantly correlated with DNA integrity. It was negatively and non significantly correlated with plasma membrane integrity and acrosome status. 8. The live sperm percentage was significantly (P<0.05) and positively correlated with plasma membrane integrity. It was positively but non significantly correlated with DNA integrity and acrosome status. 9. The plasma membrane integrity was non significantly and positively correlated with acrosome status and DNA integrity. 10. The acrosome status was positively and non significantly correlated with DNA integrity. Post- thaw characters: 11. The overall mean value of semen progressive motility and live proportion were found to be 46.33±0.36 percent and 61.53±0.76 percent respectively. The values did not vary significantly between the rams. 12. The overall mean value of intact plasma membrane integrity, intact acrosome status, and intact DNA integrity were found to be 69.66±0.67 percent, 70.94±0.79 percent and 74.58±0.79 percent respectively which did not differ significantly between the rams. 13. The difference between the ejaculate volume, mass motility, sperm concentration, live sperm percentage, plasma membrane integrity, acrosome status, and DNA integrity were found to be non significant among the rams. 14. The plasma membrane integrity was non significantly and positively correlated with acrosome status, live proportion, DNA integrity and also with progressive motility percentage. 15. The acrosome status was positively and non significantly correlated with live proportion, progressive motility percentage and DNA integrity. 16. The DNA integrity was positively and non significantly correlated with live proportion, but non significantly and negatively correlated with progressive motility percentage. 17. The live proportion was positively and non significantly correlated with progressive motility.
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    ThesisItemOpen Access
    EFFECT OF CAFFEINE CITRATE AND PROSTAGLANDINF2α AS ADDITIVES ON RAM SEMEN PRESERVATION
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2015) Mehta, Arjun Kumar; Sinha, M. P.
    The present study was conducted to evaluate the seminal attributes of six Chottanagpuri rams and to see the effect of different dilutors on preservation at refrigerator temperature. A schedule of twice weekly collection of semen per ram was followed. Ejaculates having three plus initial motility were finally diluted with TCFY extender in 1:1 ratio containing additives viz. caffeine citrate and ProstaglandinF2α at the rate of 2mM/ml, or 4 µg/ml. separately. Extended semen samples were preserved in refrigerator at 4-50C and evaluated for the various sperm characteristics viz., progressive motility, pH, live sperm percentage and sperm abnormality at 24, 48, 72 and 96 hours of preservation. Data were statistically analyzed for assessing their preservability. The results obtained have been summarized below: 1. The colour of the Chottanagpuri ram semen was found to be creamy or creamy white. 2. The mean value for different seminal attributes viz., volume (ml.), pH, mass motility, sperm concentration (millions/ml.), live sperm percentage, head, mid piece and tail abnormality percentage in neat semen was 0.69±0.01 ml, 6.66±0.02, 3.81±0.07 (0-5 score), 4043.06±106.76 millions/ml, 81.61±0.23 percent, 0.49±0.05 percent, 0.60±0.05 percent and 5.94±0.13 percent, respectively. 3. Ejaculate volume, pH, mass motility, sperm concentration and live sperm percentage ranged between 0.67±0.03 to 0.72±0.03 ml, 6.62±0.03 to 6.72±0.05, 3.66±0.24 to 4.00±0.46, 3916.67±261.62 and 4291.67±345.31 millions/ml and 81.00±0.37 to 82.17±0.75 percent respectively. 4. The difference in ejaculate volume, pH, mass motility, abnormal sperm percentage, sperm concentration and live sperm percentage were found to be non significant among the rams. 5. Addition of 2mM caffeine and 4µg PGF2α had beneficial effect in respect to progressive motility at all hours of preservation. Amongst the two additives, addition of caffeine showed significantly higher progressive motility at all hours of preservation. 6. Live sperm percentages were also higher in Caffeine and ProstaglandinF2α added diluters than control at all hours of preservation. However the difference was not significant at 72 and 96 hours of preservation. 7. Percentage of head and mid piece abnormality did not differ significantly among the control, Caffeine and ProstaglandinF2α added diluters at any hours of preservation. However the effect of additives on tail abnormality were significant at all hours of preservation which was significantly lowest in caffeine added diluters followed by PGF2α added and control dilutor.