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Birsa Agricultural University, Ranchi

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2013 - 20153
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Subject: Animal Reproduction and Gynecology × End date: 2019 × Start date: 2013 × Type: Thesis ×
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    ThesisItemOpen Access
    INFLUENCE OF ANTIOXIDANTS, REDUCED GLUTATHIONE AND VITAMIN E ON MICROSCOPIC AND OXIDATIVE STRESS PARAMETERS OF FROZEN THAWED CHHOTANAGPURI RAM SEMEN
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2014) KURMI, DHRUBA JYOTI; Singh, M. P.
    The result of the present study shows that addition of vitamin E and reduced glutathione in Tris freezing medium improved the percentage of sperm motility, live sperm, plasma membrane integrity, acrosomal integrity and DNA integrity in the frozen thawed semen compared to the Tris control as freezing medium. 2mM Vit E added in Tris dilutor appeared to be the best. Thus on the basis of the present result 2mM Vit E and 5mM GSH may be recommended for supplementation in Tris freezing medium for freezing of Chhotanagpuri ram semen. However further study on large population is required for validation of the result.
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    ThesisItemOpen Access
    “STUDIES ON CRYOPRESERVATION AND POSTTHAWED ASSESMENT OF CHHOTANAGPURI RAM SEMEN”
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2013) Toppo, Tej Pawan; Sinha, M. P.
    The studies were carried out on seminal ejaculate characteristics and its cryopreservation of Chhotanagpuri rams. The main purpose of this study was to determine the cryotolerance of Chhotanagpuri ram spermatozoa on the basis of post-thaw semen quality. The results obtained have been discussed earlier in detail, the same have been summarized as follows: Seminal characters at pre- freeze stage: 1. The colour of Chhotanagpuri ram semen was found to be creamy or creamy white. 2. The overall mean ejaculate volume, mass motility, sperm concentration and live sperm percentage were found to be 0.56±0.02 ml, 4.28±0.08, 4210.42±29.00 millions/ml and 84.50±0.65 percent respectively. 3. The overall mean value of intact plasma membrane integrity, intact acrosome status and intact DNA integrity were found to be 78.11±0.63 percent, 83.42±0.75 percent and 89.97±0.38 percent respectively. 4. Mean live percentage, intact plasma membrane integrity, intact acrosome, and intact DNA integrity percentage were significantly higher in neat semen samples than frozen thawed semen. 5. The ejaculate volume was negatively and non significantly correlated with sperm concentration, acrosome status and DNA integrity .The ejaculate volume was significantly (P<0.05) and positively correlated with mass motility. It was positively but non significantly correlated with live sperm % and plasma membrane integrity. 6. The mass motility was significantly (P<0.01) and positively correlated with sperm concentration, live sperm percentage. Its correlation with acrosome status, plasma membrane integrity and DNA integrity was positive but non significant. 7. The sperm concentration was highly significantly (P<0.01) and positively correlated with live sperm percentage whereas it was positively and non significantly correlated with DNA integrity. It was negatively and non significantly correlated with plasma membrane integrity and acrosome status. 8. The live sperm percentage was significantly (P<0.05) and positively correlated with plasma membrane integrity. It was positively but non significantly correlated with DNA integrity and acrosome status. 9. The plasma membrane integrity was non significantly and positively correlated with acrosome status and DNA integrity. 10. The acrosome status was positively and non significantly correlated with DNA integrity. Post- thaw characters: 11. The overall mean value of semen progressive motility and live proportion were found to be 46.33±0.36 percent and 61.53±0.76 percent respectively. The values did not vary significantly between the rams. 12. The overall mean value of intact plasma membrane integrity, intact acrosome status, and intact DNA integrity were found to be 69.66±0.67 percent, 70.94±0.79 percent and 74.58±0.79 percent respectively which did not differ significantly between the rams. 13. The difference between the ejaculate volume, mass motility, sperm concentration, live sperm percentage, plasma membrane integrity, acrosome status, and DNA integrity were found to be non significant among the rams. 14. The plasma membrane integrity was non significantly and positively correlated with acrosome status, live proportion, DNA integrity and also with progressive motility percentage. 15. The acrosome status was positively and non significantly correlated with live proportion, progressive motility percentage and DNA integrity. 16. The DNA integrity was positively and non significantly correlated with live proportion, but non significantly and negatively correlated with progressive motility percentage. 17. The live proportion was positively and non significantly correlated with progressive motility.
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    ThesisItemOpen Access
    EFFECT OF CAFFEINE CITRATE AND PROSTAGLANDINF2α AS ADDITIVES ON RAM SEMEN PRESERVATION
    (Birsa Agricultural University, Kanke, Ranchi, Jharkhand, 2015) Mehta, Arjun Kumar; Sinha, M. P.
    The present study was conducted to evaluate the seminal attributes of six Chottanagpuri rams and to see the effect of different dilutors on preservation at refrigerator temperature. A schedule of twice weekly collection of semen per ram was followed. Ejaculates having three plus initial motility were finally diluted with TCFY extender in 1:1 ratio containing additives viz. caffeine citrate and ProstaglandinF2α at the rate of 2mM/ml, or 4 µg/ml. separately. Extended semen samples were preserved in refrigerator at 4-50C and evaluated for the various sperm characteristics viz., progressive motility, pH, live sperm percentage and sperm abnormality at 24, 48, 72 and 96 hours of preservation. Data were statistically analyzed for assessing their preservability. The results obtained have been summarized below: 1. The colour of the Chottanagpuri ram semen was found to be creamy or creamy white. 2. The mean value for different seminal attributes viz., volume (ml.), pH, mass motility, sperm concentration (millions/ml.), live sperm percentage, head, mid piece and tail abnormality percentage in neat semen was 0.69±0.01 ml, 6.66±0.02, 3.81±0.07 (0-5 score), 4043.06±106.76 millions/ml, 81.61±0.23 percent, 0.49±0.05 percent, 0.60±0.05 percent and 5.94±0.13 percent, respectively. 3. Ejaculate volume, pH, mass motility, sperm concentration and live sperm percentage ranged between 0.67±0.03 to 0.72±0.03 ml, 6.62±0.03 to 6.72±0.05, 3.66±0.24 to 4.00±0.46, 3916.67±261.62 and 4291.67±345.31 millions/ml and 81.00±0.37 to 82.17±0.75 percent respectively. 4. The difference in ejaculate volume, pH, mass motility, abnormal sperm percentage, sperm concentration and live sperm percentage were found to be non significant among the rams. 5. Addition of 2mM caffeine and 4µg PGF2α had beneficial effect in respect to progressive motility at all hours of preservation. Amongst the two additives, addition of caffeine showed significantly higher progressive motility at all hours of preservation. 6. Live sperm percentages were also higher in Caffeine and ProstaglandinF2α added diluters than control at all hours of preservation. However the difference was not significant at 72 and 96 hours of preservation. 7. Percentage of head and mid piece abnormality did not differ significantly among the control, Caffeine and ProstaglandinF2α added diluters at any hours of preservation. However the effect of additives on tail abnormality were significant at all hours of preservation which was significantly lowest in caffeine added diluters followed by PGF2α added and control dilutor.