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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan
Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry.
The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services.
The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.
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ThesisItem Open Access IMPACT OF WEATHER ON PHENOLOGY OF MUSTARD (Brassica juncea L.) AND ITS EFFECT ON INSECT POLLINATORS DIVERSITY AND POLLINATION(UHF,NAUNI, 2017-12) MANJU, DEVI; SHARMA, HARISH KUMARABSTRACT The present study was conducted to determine the impact of weather on phenology of mustard (Brassica juncea L.) and its effect on insect pollinators diversity and pollination. The studies were conducted in mustard crop by inducing the plant to flower early or late through different sowing dates. The crop was sown during two major seasons first from October- November (season 1) and second from February to March (season 2) in three different dates in each season at an interval of fifteen days. Season 1 and season 2 had significant effect on flower phenology, insect pollinators diversity and relative abundance of insects and seed set of mustard crop. Flower bud development was significantly more in mustard crop grown in season 1 (11.12 days) as compared to season 2 (8.55 days). Season 1 had longest flowering duration (39 days). Flower phenology characters like duration of flowering, days to flowering, longevity of flower, pollen viability and nectar sugar concentration have been found sensitive to change in weather conditions like temperature, relative humidity and rainfall. Flower bud development, longevity of flower was positively correlated with temperature. Nectar sugar concentration decreased with decreasing relative humidity and had a direct correlation with air temperature. Cool temperature at the time of flowering favoured nectar flow. Open mode of pollination was superior to hand pollination and pollinators exclusion. Higher seed set percentage was observed in open pollination (66.87 %) as compared to hand pollination (51.55 %) and pollinator’s exclusion (42.68 %) during both the seasons. Seed set in mustard was higher in season 1 (62.15 %). Delayed sowing of mustard resulted into reduction in the seed set due to short flowering duration, low nectar sugar concentration, which were affected by weather conditions. The population of insect visitors was higher in season 1 as compared to season 2. A total of 88 insects belonging to 63 genera under 31 families and 9 orders were found to visit the mustard bloom in season 1. Forty one insects belonging to 23 families and 9 orders were observed in season 2. Order Hymenoptera contributed higher percentage of the insect visitors. Hive bees (Apis cerana F. and A. mellifera L.) were the most efficient pollinators of mustard. High temperature, heavy rainfall and low temperature had negative influence on abundance of insect visitors on mustard flower. Shannon Weiner pollinator diversity indices indicated that for sampling pollinator diversity, all the methods have to be collectively employed as no single method is fully reliable.ThesisItem Open Access BIOMASS PRODUCTION, NUTRIENT CYCLING AND CARBON SEQUESTRATION POTENTIAL OF BAMBOO PLANTATIONS IN MID-HILLS, SUB-MONTANE AND LOW HILLS OF HIMACHAL PRADESH(UHF,NAUNI, 2017-12) YOURMILA, KUMARI; BHARDWAJ, D.R.ABSTRACT The present investigations entitled “Biomass production, nutrient cycling and carbon sequestration potential of bamboo plantations in mid-hills, sub-montane and low hills of Himachal Pradesh” were carried out at three sites of Dr YSP University of Horticulture and Forestry, Nauni-Solan, Himachal Pradesh viz., experimental field of Department of SAF at Nauni, comprising of 7 bamboo species under pure bamboo plantation and 3 bamboo species under bamboo based agroforestry system in mid-hill conditions, RHRS Jacch, Kangra comprising of 5 bamboo species under bamboo based agroforestry system in sub-montane conditions and RHRS Bajaura, Kullu comprising of 4 tree species in combination with Phyllostaychys pubescens, during 2015-2016. The results revealed that Dendrocalamus hamiltonii showed better growth and development in pure bamboo plantation under mid-hill conditions. Whereas, in bamboo based agroforestry system, Bambusa balcooa displayed better growth behavior than Dendrocalamus asper and Dendrocalamus hamiltonii. Under submontane low hill conditions, the growth was better in Bambusa bambos when planted in agroforestry system while, Phyllostachys pubescens showed better growth and development behaviour when planted under the canopy of Platanus orientalis. The biomass of different bamboo species was affected significantly due to variation in climatic conditions of HP. In pure bamboo plantation under mid-hill conditions, Dendrocalamus asper produced maximum biomass whereas, in bamboo based agroforestry system, Dendrocalamus hamiltonii produced maximum biomass. In bamboo based agroforestry system under sub-montane and low-hill conditions, Bambusa bambos showed highest biomass production while, Phyllostachys pubescens produced highest biomass when planted with Platanus orientalis. Irrespective of bamboo species, the biomass production enhanced with increasing culm age. Dendrocalamus asper, Dendrocalamus hamiltonii and Bambusa bambos showed better carbon sequestration potential under mid-hill and sub-montane conditions, respectively. Under mid hill conditions, Dendrocalamus asper showed better nutrient cycling in pure plantation as well as in bamboo based agroforestry system while, under sub-montane low hill conditions, Bambusa bambos showed better nutrient cycling under bamboo based agroforestry system. In temperate climatic conditions, better nutrient status was recorded in soil under Phyllostachys pubescens and Platanus orientalis. Except for soil pH, all the soil physico-chemical and microbiological parameters decreased with increase in soil depth.ThesisItem Open Access PRODUCTION AND CHARACTERIZATION OF LYTIC ENZYMES FROM Pseudomonas SPECIES ASSOCIATED WITH APPLE(UHF,NAUNI, 2017-12) KUMARI, MANORMA; KAUR, MOHINDERABSTRACT The aim of present study was production and characterization of lytic enzymes i.e. protease and chitinase produced by fluorescent Pseudomonas sp. isolated from the normal and replant sites of apple orchards. Eleven Pseudomonas sp. were isolated from the replant sites of Rauni and Maggota of Shimla district. Four isolated strains of fluorescent Pseudomonas sp. from replant sites of apple were also used for screening of direct and indirect plant growth promoting activities like lytic enzymes (protease & chitinase), siderophores, phosphate solubilization and production of HCN, ammonia, plant growth regulators (auxins, cytokinins and gibberellins), antifungal and antibacterial activity. By 16S rRNA gene characterization, isolates Rn1 and Mgn1 showed 99.0% and 97.0% similarity with Pseudomonas brassicacearum KU139391 and Pseudomonas aeruginosa respectively. The pathogenic fungal isolates from replant sites of orchard at Rauni i.e. RF3 showed 99.0% similarity with Phytopythium litorale and from Maggota i.e. MF5 showed 99.0% similarity with Pythium ultimum by ITS gene sequencing. Optimization of media, time of incubation, temperature, pH, carbon, nitrogen and mineral sources were done for the production and characterization of protease & chitinase from four Pseudomonas strains L (Pseudomonas putida), M (Pseudomonas fluorescens), G and H (Pseudomonas aeruginosa), which were also used for mass multiplication of inoculum for field trials. Purification of protease and chitinase was done through Sephadex G-75 column chromatography. Through SDS-PAGE electrophoresis, the molecular weight of Protease was recorded as 31.0 to 43kDa and 26.0 to 38.0kDa for chitinase. This study also reported significant role of protease & chitinase in antagonistic activity against Dematophora necatrix, Phytopythium litorale and Pythium ultimum. Lytic enzymes (protease and chitinase) are responsible for the lysis and hyperparasitism of antagonists against deleterious fungal and bacterial pathogens. Lytic enzymes along with other plant growth promoting activities may be the reasons for better establishment and growth promotion of replanted apple. Treatments with bioformulations of consortia were found to be more effective in growth and establishment of replanted apple. Therefore these strains can be exploited for the management of replant problem of apple.ThesisItem Open Access MORPHOGENETIC VARIATION OF SEABUCKTHORN (Hippophae rhamnoides L.) IN SPITI VALLEY OF HIMACHAL PRADESH(UHF,NAUNI, 2017-12) KAIRON, VINOD KUMAR; SANKHYAN, H.P.ABSTRACT The study entitled “Morphogenetic Variation of Seabuckthorn (Hippophae rhamnoides L.) in Spiti Valley of Himachal Pradesh” was conducted during the year 2014-17 with the aim to evaluate morphological variation, variation in fodder quality traits, variation in physical and chemical characteristics of berries, seed oil and deoiled cake and and molecular characterization of morphovariants using RAPD of different gene pools of Hippophae rhamnoides under different gene pool areas and growing conditions. Nine gene pool areas and three growing conditions within gene pool areas selected for the study showed wide variation among different morphological, fodder quality traits, physical and chemical characteristics of berries, seed oil and deoiled cake. Morphological traits (plant height, leaf length, leaf width, leaf area, leaf density, no. of thorn, 100 fruits fresh weight, fruit diameter and 100 seed weight) differed positively significant among the gene pool areas and growing conditions, except branch length. All proximate leaf composition (100 leaf fresh weight, 100 leaf dry weight, crude fiber, crude protein and total ash) and mineral nutrient (nitrogen, potassium, calcium and magnesium) compositions showed significant difference between the gene pool areas and growing conditions for all the parameters except for leaf dry matter content and phosphorus. All physical and chemical characteristics of berries (pulp%, ascorbic acid and total sugar content), per cent seed oil, physico-chemical traits of oil (specific gravity, acid value, iodine value and saponification value) and deoiled seed cake (protein content, starch content and total sugar content) showed significant under different gene pool areas and growing conditions for all the traits, except for physical characteristics (refractive index and specific gravity) of seed oil. The RAPD fragments obtained after the amplification of genomic DNA for 27 genotypes. Total 89 RAPD markers were amplified and 71 out of 89 were found to be polymorphic in nature. The size range of polymorphic bands was 100 bp to 2,500 bp. The Jaccard's similarity correlation coefficient values ranged from 0.520 to 0.879. The highest value (0.879) was found between 87.9 per cent was found between GPA3GC1 and GPA3GC2 followed by 0.853 between GPA4GC1 and GPA8GC1 genotypes. In overall, GPA4 (Schilling) gene pool area proved to be the best among different gene pool areas on the basis of fodder quality traits. GPA3 (Sheigo) was found to be best in all gene pool areas for physical and chemical characteristics of berries, seed oil and deoiled seed cake. Among different growing conditions, GC3 (Crop Land) growing condition was found to be top ranking for most of morphological traits, fodder quality traits, and physical and chemical characteristics of berries, seed oil and deoiled seed cake. So Seabuckthorn can be used as agroforestry crop in different systems and designs. Based on the morphological variation, variation in fodder quality traits, variation in physical and chemical characteristics of berries, seed oil and deoiled cake observations it was evident that large diversity exists in naturally growing populations of Hippophae rhamnoides in the Spiti Valley of Himachal Pradesh, and this morphological variation can be exploited for the further selection of suitable genotypes to accelerate the domestication process of this important species.ThesisItem Open Access STUDIES ON IDENTIFICATION AND SOCIO-ECONOMIC STATUS OF EXISTING AGROFORESTRY SYSTEMS IN SIRMAUR DISTRICT OF HIMACHAL PRADESH(UHF,NAUNI, 2017-12) TIWARI, PRABHAT; PANT, K.S.ABSTRACT The present investigation entitled “Studies on identification and socio-economic status of existing agroforestry systems in Sirmaur district of Himachal Pradesh” was carried out in Sirmaur district which is located between 30°22'30"–31°01'20" N latitude and 77°01'12"–77°49'40" E longitude in Himachal Pradesh. The climate in this area is sub-tropical to temperate depending upon the elevation. The inclusion of woody perennials within farming systems, has been practiced as a traditional land use and livelihood option since time immemorial. For the study, Sirmaur district were selected and in district three altitudinal zones were prepared, such as altitudinal zone I (<1000m amsl), zone II (1000-2000m amsl), zone III (>2000m amsl) and in each altitudinal zone, four panchayats were selected and in each selected panchayats, three farmers category were made namely, marginal (<1 ha), small (1-2 ha) and medium (2-5 ha). Finally from each farmer category five farming families were taken for the study. Socioeconomic status of farmers, vegetation composition, biomass, economic viability and technological constraints in the system have been studied during the research. The average family size of sampled households were 6.90, 5.67 and 7.35 persons/household with average literacy rate 85.09 to 88.29 per cent ranging from altitude zone I to altitude zone III. The results found that prevalent agroforestry systems in study area were AS, ASH, AH, ASP, PS, SP and PSH, among these system types most predominant were AS, ASH and AH systems. The maximum above ground biomass production in three altitudinal zones was noticed in ASH under zone I (25.82 t ha-1), while in three farmers category, it was maximum in ASH with small farmers (22.81 t ha-1). Higher above ground biomass production of pastoral agroforestry systems among three altitudinal zone was recorded in SP under zone III (22.86 t ha-1) whereas, in three farmers category was recorded maximum in SP under marginal farmers (23.11 t ha-1). Similar trends were also noticed in the below ground and total biomass production. Maximum net returns among three altitudinal zones was reported in AH under zone III (Rs. 3,37,716/-), while in three farmers category, it was recorded maximum in AH under small farmers(Rs. 2,46,070/-). In pastoral agroforestry systems highest net profit among three altitudinal zones was noticed in ASP under zone I (Rs. 82,675/-), on the other hand in three farmers category was recorded higher net profit was recorded in ASP under marginal farmers (Rs. 82,746/-). Benefit-Cost ratio of agroforestry systems among three altitudinal zones was noticed highest in AH under zone III (2.37), while in three farmers category was recorded maximum in AH under medium farmers (2.27). Pastoral agroforestry systems was observed highest BC ratio in PSH under zone II (2.20), on the other hand among three farmers category, it was found maximum in PSH under small farmers (2.21). Technological gaps identifying the socio-economic and other constraints have highlighted the inherent weakness of existing AF systems. The most prominent among these constraints includes disproportionate applications of insecticides/pesticides and fertilizers, fragmented landholdings, lack of efficient marketing channels, communication gap between lab to land and ineffective implementation of government sponsored schemes for the benefits of farming communities. Relevant agroforestry solutions include conducting location specific diagnostic survey, strengthening of agro-processing facilities, voluntarily adoption of some villages by state agriculture universities and other related institutions and opening of govt. authorized sale centers at accessible places etc. From the above study it is concluded that AH system type found to be best for economic point of view, hence these systems may be recommended to the Sirmaur district of Himachal Pradesh.ThesisItem Open Access INFLUENCE OF INTEGRATED NUTRIENT MANAGEMENT ON YIELD AND SOIL PROPERTIES IN PEA- TOMATO CROPPING SEQUENCE(UHF,NAUNI, 2017-12) MANJU, KUMARI; TRIPATHI, D.ABSTRACT The present investigation entitled “Influence of integrated nutrient management on yield and soil properties in pea- tomato cropping sequence” was conducted during 2014-15 and 2015- 16 at the experimental farm of Department of Soil Science and Water Management, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni- Solan, H.P. The experiment was laid out in randomized block design with nine treatments and three replications. The experiments were conducted for two years in pea and tomato cropping sequence where in, recommended dose of fertilizer was substituted with organic at three levels (10, 20 and 30 per cent). The higher rates of fertilizer in the tune of 10, 20 and 30 per cent over recommended dose has also been tried to arrive at concrete and conclusive results. Integrated use of inorganic fertilizer along with FYM, vermicompost and PGPR improved physico-chemical, microbiological properties of soil and yield of pea and tomato cropping sequence. The addition of 80% NPKM + 20% N through FYM and VC (50:50) (T3) recorded highest (162.27 q/ha-Pea, 606.51 q/ha –Tomato) crop yield. The increase in yield of pea and tomato crops was found to be 23.29 and 29.60 per cent over recommended dose of fertilizers (T5), respectively. Higher uptake of major nutrients and good quality of pea and tomato fruits has also been recorded with 80% NPKM + 20% N through FYM and VC (50:50) treatment. The higher B:C ratio (3.08:1 and 5.48:1) was found with the substitution (up to 20 %) inorganic fertilizers with organics. Thus, a saving of about 20 per cent inorganic fertilizer can be achieved through organic substitution besides appreciable improvement in physico-chemical properties, microbiological properties, crop yield and quality of pea-tomato.ThesisItem Open Access ASSOCIATION GENETICS IN POPULUS DELTOIDES(UHF,NAUNI, 2017-12) THAKUR, SHIKHA; THAKUR, SANJEEVABSTRACT The present investigation entitled “Association genetics in Populus deltoides” was carried out in the Department of Tree Improvement and Genetic Resources, Dr Y. S. Parmar University of Horticulture & Forestry Nauni, Solan (HP) during 2013-2016. There is a collection of Populus deltoides clones procured from different countries namely USA, Australia and parts of India and maintained in the germplasm block of Naganji nursery from which ninety five clones were included in the study. Cuttings were raised in the nursery in RBD with three replications at 50 cm X 50 cm spacing and were evaluated for morphological characters in field as well as wood and molecular studies in laboratory. Analysis of variance revealed that all morphological traits were significantly different in nature. On the basis of clustering of morphological and wood traits, ninety five clones were distributed into five distinct clusters suggesting the presence of different sources of relationships amongst the clones. The gene action study revealed that additive variance was observed more than the dominance variance for most of the parameters studied except for growth traits. Growth traits expressed highly significant and positive genotypic as well as phenotypic correlation. Ninety five clones were tested for their genetic diversity using hundred SSR markers out of which ninety three gave results and sixteen ISSR markers out of which ten markers gave results. Results disclosed that dendrogram generated from ninety three SSR markers divided whole population of ninety five clones into two major clusters whereas dendrogram generated from ISSR markers depicted that whole population was divided into three major clusters. The structure analysis from SSR data revealed that the population gets divided into two sub-populations whereas from ISSR data, it was clear that the population gets divided into three sub-populations indicating the level of diversity existing in the population. On the basis of population structure analysis it is concluded that this clonal population structure is diverse. Results from genetic diversity analysis as well as STRUCUTRE analysis showed that the clustering pattern of the genotypes in STRUCUTRE analysis were almost similar to the splitting in the UPGMA tree which reveals that all the genotypes were clustered better with two clusters in SSR markers and three clusters in case of ISSR markers. Association studies of commercially important wood traits were conducted using ISSR and SSR marker data. Some important associations of markers with specific gravity of poplar wood were found in case of SSR markers whereas with ISSR markers, various associations of holocellulose and lignin content were obtained.ThesisItem Open Access IMPACT OF WEATHER AND ORCHARD MANAGEMENT PRACTICES ON APPLE PRODUCTIVITY AND SOIL HEALTH IN KULLU DISTRICT OF HIMACHAL PRADESH(UHF,NAUNI, 2017-12) SHARMA, PRIYANKA; MOHINDER, SINGHABSTRACT The present investigation entitled “Impact of weather and orchard management practices on apple productivity and soil health in Kullu district of Himachal Pradesh” was conducted during the years 2015 and 2016 at four altitudinal gradients/locations, viz. Bajaura (1090m amsl), Seobagh (1189m amsl), Naggar (1880m amsl) and Arsu (2080 m amsl), on three commercial apple varieties viz. Red Delicious, Royal Delicious and Golden Delicious. The different phenological stages (Silver tip, Green tip, Pink bud, Full bloom and Petal fall) in apple were observed at earliest at Bajaura, where the apple crop experienced higher average temperature compared to other locations. Minimum number of days for silver tip stage (54.22 days with 349.22 GDD), green tip stage (71.67 days with 484.00 GDD), pink bud stage (86.94 days with 662.54 GDD), full bloom stage (93.89 days with 749.67 GDD) and for petal fall stage (99.00 days with 807.48 GDD) were observed at Bajaura, followed by Seobagh, Naggar and Arsu. Among different cultivars, Red Delicious cultivar took minimum number of days for silver tip stage (56.58 days and 264.93 GDD), green tip stage (74.92 days with 387.44 GDD), pink bud stage (89.00 days with 526.85 GDD), full bloom stage (95.71 days with 598.33 GDD) and for petal fall stage (101.58 days with 662.14 GDD), followed by Royal Delicious and Golden Delicious. The studies on long term impact of weather parameters on apple productivity revealed that the minimum temperature during dormancy stage (Jan- March) exhibited positive and significant correlation with productivity of apple. Whereas, maximum temperature during April- June (flowering, fruit set and fruit development stage) showed negative and significant impact on apple productivity. The studies on effect of management practices and orchard aspect on soil health at different locations revealed maximum soil pH (6.77) and OC (2.92 %) in low input orchards when compared with high input orchards. Among different locations highest soil pH and EC were found in Arsu, followed by Naggar, Seobagh and Bajaura. High input orchards showed higher values of soil macronutrients, N (330.4 kg/ha), P (36.64 kg/ha) and K (287.4 kg/ha) than low input orchards. Soil micronutrients Fe, Zn and Cu were also found higher in high input orchards. In low input orchards, soil microbial count and biomass were recorded higher than high input orchards. The orchards in south west aspect recorded higher N (309.7 kg/ha) and Mn (27.60 mg/kg) as compared to orchards in north east aspect. Whereas, other soil parameters were found higher in north east than south west aspect.ThesisItem Open Access STUDIES ON MORPHO-CHEMICAL VARIATION IN GYNODIOECIOUS Valeriana jatamansi JONES(UHF,NAUNI, 2017-12) THAKUR, PANCY; SHARMA, YASH PALABSTRACT The present investigation entitled “Studies on morpho-chemical variation in gynodioecious Valeriana jatamansi Jones” was carried out in the field and laboratory of the Department of Forest Products, College of Forestry, Dr. Y S Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) during 2014-16. The study was conducted under five broad heads namely (i) Morphological features and isolation of morphovariants, (ii) Stability of morphological features of morpho-variants, (iii) Evaluation of different morphovariants for phytochemical content and yield, (iv) Progeny evaluation of female & and bisexual plants, and (v) Standardization of analytical method for quantification of valepotriates. Already existing plants of different germplasms i.e. IVth inbred generation of INGR 11036 (IC 05844665), IVth inbred generation of “A” germplasm and general plants of different populations of Valeriana jatamansi were utilized for the present study. Wide variation in the plants for plant habit, leaf characteristics and floral parameters was observed. Detailed morphological studies resulted in identification of 46 morpho-variants differing in atleast one distinct qualitative morphological feature. Out of 46 selected morpho-variants, 25 morpho-variants were female flowering plants and 21 morpho-variants were bisexual flowering plants. The distinct qualitative morphological features of morpho-variants were found stable in the vegetatively raised offsprings of all the morpho-variants. Significant variation was recorded among the morpho-variants with regard to leaf length, leaf width, ratio of leaf length and leaf width, plant height, petiole length, petiole diameter, rhizome diameter, fresh & dry aerial biomass, fresh & dry rootstock biomass and flowering initiation. Among female and bisexual morpho-variants, significant difference was observed for plant height, leaf width, petiole diameter and fresh and dry biomass of rhizomes. Phytochemical analysis revealed wide variation in concentration of valepotriate’s (valtrate, acevaltrate, Didrovaltrate & IVHD Valtrate) in roots, rhizomes & rootstocks of different morpho-variants. The valtrate content varied from 0.749% to 2.878% in rhizomes, 0.917% to 5.221% in roots and 0.927% to 3.267% in rootstock; acevaltrate content varied from 0.022% to 0.512% in rhizomes, 0.015% to 1.647% in roots and 0.260% to 0.909% in rootstock; didrovaltrate content varied from 0.187% to 1.688% in rhizomes, 0.053% to 0.365% in roots and 0.136% to 0.958% in rootstock; IVHD valtrate content varied from 0.179% to 1.491% in rhizomes, 0.176% to 0.755% in roots and 0.214% to 1.101% in rootstock of different morpho-variants. The total valepotriate’s content varied between 1.761% to 4.848% in rhizomes, 1.598% to 6.420% in roots and 1.845% to 4.875% in rootstock of different morpho-variants. The concentration of diene type of valepotriate’s was more in roots than rhizomes and monoene type of valepotriate’s were more in rhizomes than roots. On the basis of phytochemical content and morphological studies, promising morpho-variants has been identified which can be further used for breeding purpose for isolation of chemotype/variety with stable and distinct morphological features. Seasonal variation studies indicated that during September month, valepotriates content was maximum but due to higher rootstock biomass, valepotriate’s yield was maximum in November month. Analytical method for quantification of individual valepotriate’s through HPLC has been standardized and developed.