STUDIES ON MORPHO-CHEMICAL VARIATION IN GYNODIOECIOUS Valeriana jatamansi JONES
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Date
2017-12
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UHF,NAUNI
Abstract
ABSTRACT
The present investigation entitled “Studies on morpho-chemical variation in gynodioecious Valeriana
jatamansi Jones” was carried out in the field and laboratory of the Department of Forest Products, College of
Forestry, Dr. Y S Parmar University of Horticulture and Forestry, Nauni, Solan (H.P.) during 2014-16. The
study was conducted under five broad heads namely (i) Morphological features and isolation of morphovariants,
(ii) Stability of morphological features of morpho-variants, (iii) Evaluation of different morphovariants
for phytochemical content and yield, (iv) Progeny evaluation of female & and bisexual plants, and (v)
Standardization of analytical method for quantification of valepotriates. Already existing plants of different
germplasms i.e. IVth inbred generation of INGR 11036 (IC 05844665), IVth inbred generation of “A” germplasm
and general plants of different populations of Valeriana jatamansi were utilized for the present study. Wide
variation in the plants for plant habit, leaf characteristics and floral parameters was observed. Detailed
morphological studies resulted in identification of 46 morpho-variants differing in atleast one distinct qualitative
morphological feature. Out of 46 selected morpho-variants, 25 morpho-variants were female flowering plants
and 21 morpho-variants were bisexual flowering plants. The distinct qualitative morphological features of
morpho-variants were found stable in the vegetatively raised offsprings of all the morpho-variants. Significant
variation was recorded among the morpho-variants with regard to leaf length, leaf width, ratio of leaf length and
leaf width, plant height, petiole length, petiole diameter, rhizome diameter, fresh & dry aerial biomass, fresh &
dry rootstock biomass and flowering initiation. Among female and bisexual morpho-variants, significant
difference was observed for plant height, leaf width, petiole diameter and fresh and dry biomass of rhizomes.
Phytochemical analysis revealed wide variation in concentration of valepotriate’s (valtrate, acevaltrate,
Didrovaltrate & IVHD Valtrate) in roots, rhizomes & rootstocks of different morpho-variants. The valtrate
content varied from 0.749% to 2.878% in rhizomes, 0.917% to 5.221% in roots and 0.927% to 3.267% in
rootstock; acevaltrate content varied from 0.022% to 0.512% in rhizomes, 0.015% to 1.647% in roots and
0.260% to 0.909% in rootstock; didrovaltrate content varied from 0.187% to 1.688% in rhizomes, 0.053% to
0.365% in roots and 0.136% to 0.958% in rootstock; IVHD valtrate content varied from 0.179% to 1.491% in
rhizomes, 0.176% to 0.755% in roots and 0.214% to 1.101% in rootstock of different morpho-variants. The total
valepotriate’s content varied between 1.761% to 4.848% in rhizomes, 1.598% to 6.420% in roots and 1.845% to
4.875% in rootstock of different morpho-variants. The concentration of diene type of valepotriate’s was more in
roots than rhizomes and monoene type of valepotriate’s were more in rhizomes than roots. On the basis of
phytochemical content and morphological studies, promising morpho-variants has been identified which can be
further used for breeding purpose for isolation of chemotype/variety with stable and distinct morphological
features. Seasonal variation studies indicated that during September month, valepotriates content was maximum
but due to higher rootstock biomass, valepotriate’s yield was maximum in November month. Analytical method
for quantification of individual valepotriate’s through HPLC has been standardized and developed.
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