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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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20121
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Subject: Biotechnology and Molecular Biology × Author: RAJNI, KUMARI × Start date: 1994 × Type: Thesis ×
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    ThesisItemOpen Access
    GENETIC FIDELITY OF MICROPROPAGATED PLANTS OF CARNATION (Dianthus caryophyllus L.)
    (2012) RAJNI, KUMARI; SHARMA, S.K.
    ABSTRACT The present investigation entitled “ Genetic fidelity of micropropagated plants of carnation (Dianthus caryophyllus L.)” was carried out to study the genetic similarity among the plants micropropagated through different technique of micropropagation viz. axillary buds, suspension and callus culture through RAPD markers. All types of shoots were multiplied on already standardized medium containing MS medium supplemented with 1.0 mg l-1 BA which was solidified with 1.0% agar in order to lessen the problem of vitrification. In vitro raised shoots were rooted in ½ strength MS medium supplemented with 2 mg l-1 IBA and 2 g l-1 activated charcoal and hardened successfully. Analysis of genomic DNA of mother plant and fifteen in vitro raised well acclimatized plants generates 35 Scorable bands, out of which 32, 28 and 29 bands were monomorphic among vegetativaly propagated (97.14%), suspension culture (80.00%) and callus culture (85.72%) derived plants respectively and 1, 7 and 5 polymorphic bands among vegetativaly propagated (2.86%), suspension culture (20.00%) and callus culture (14.28%) derived plants respectively. The presences of polymorphic fragments among regenerants indicate that genomic alteration occurred during long term culture of the cell. Similarity co-efficient value ranged from 0.97-1.00 for mother and axillary derived plants, 0.82-1.00 for mother and suspension culture derived plants and 0.85-1.00 for mother and callus derived plants. Hence, the regeneration system from organized meristem, such as shoot tip and axillary buds are considered to be most efficient method of micropropagation than others. Although plants derived from axillary buds showed the stability but not always genetically true to the type. Hence it is imperative to regularly check the genetic fidelity of micropropagated plants while using different techniques of micropropagation.