Thumbnail Image

Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

Browse

2009 - 200912010 - 20181
Reset filters
Subject: Biotechnology × Author: SHARMA, NEHA × Thesis Type: M.Sc ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    BIOSYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF IRON NANOPARTICLES SYNTHESIZED BY Pseudomonas putida STRAIN LUA 15.1
    (UHF,NAUNI, 2018-01) SHARMA, NEHA; SHIRKOT, POONAM
    ABSTRACT Nanotechnology is gaining tremendous incentive in the present century due to its capability of modulating metals into their nanosize, which changes the chemical, physical and optical properties of metals. The synthesis of nanostructured materials, especially metallic nanoparticles, has gained utmost interest over the few decades owing to their unique properties that make them applicable in different fields of science and technology. The biological method of nanoparticles synthesis is a relatively simple, cheap and environmentally friendly than the conventional chemical and physical methods of synthesis. Aim of the present study was procurement of Pseudomonas putida strain LUA15.1, conformation of the original strain using morphological, biochemical and molecular techniques and its quantitative evaluation for iron nanoparticles synthesis. Different physicochemical parameters were optimized for synthesis of iron nanoparticles by Pseudomonas putida strain LUA15.1 using one variable at a time. Maximum iron nanoparticles synthesis was achieved at 35OC, pH: 8.0 and after 24 hrs of incubation with 5.0g/l peptone, 3.0g/l beef extract, 3 % inoculum size and 2.0mM ferrous sulfate as substrate. In vitro synthesis of iron nanoparticles was carried and biosynthesized iron nanoparticles were characterized using various spectroscopic and microscopic techniques UV-vis spectroscopy exhibited a characteristic peak at 350nm, FTIR spectrum revealed presence of of alchol-phenol, alkenes, aromatic, alkane, alkyl halide, amine, and ether biomolecules. XRD results confirmed that these nanoparticles were originally iron nanoparticles. Scanning electron microscope showed that iron nanoparticles were both monodispersed in aggregates with varation in shape triangular, square rectangular and rod, whereas shape and DLS graph revealed average diameter of iron nanoparticles was 75nm. Antibacterial activity of biosynthesized iron nanoparticles was assessed against five pathogenic bacteria, Agrobacterium tumefaciens, Pectobacterium carotovorum, Xanthomonas axonopodis, Ralstonia solanacearum and Escherichia coli under in vitro conditions and maximum zone of inhibition of 1.7 cm was observed for Agrobacterium tumefaciens. Pseudomonas putida strain LUA15.1 and its extracellularly synthesized iron nanoparticles showed significant potential for detoxification of four textile dyes viz., methylene blue, congo red, malachite green and brilliant green maximum decolorization was observed for 20 ppm methylene blue dye with 99.1% decolorization. Phytotoxity effect of methylene blue dye on five agriculturally important crops viz., Allium cepa, Brassica rapa, Solanum lycopersicum, Spinacia oleracea and Vigna radiate was investigated and it has been observed that maximum germination of 92%, maximum shoot length of 7.7cm and maximum root length 5.6 cm were in Spinacia oleracea, Allium cepa and Solanum lycopersicum respectively with ironnanoparticle treated dye.
  • Thumbnail Image
    ThesisItemOpen Access
    Studies on genomics and proteomics of a potyvirus infecting summer squash (Cucurbita pepo L.) in H.P
    (UHF,NAUNI,SOLAN, 2009) SHARMA, NEHA; BHARDWAJ, S.V.
    Abstract Summer Squash (Cucurbita pepo L.) is one of the important vegetable crops of family Cucurbitaceae. Zucchini yellow mosaic virus (ZYMV; Family: Potyviridae, Genus: Potyvirus) causes great losses to both crop and ornamental cucurbit crop production. In the present study, after detection using ELISA, molecular characterization of ZYMV (at genomic and proteomic level) infecting summer squash was carried out. A cDNA of approximately 700bp was amplified from infected tissue with the help of primers specific for potyvirus group. The PCR amplified product was sequenced and analyzed. The sequence of partial coat protein of 154 nucleotides of Una (Indian) isolate of zucchini yellow mosaic virus (ZYMV) was determined and translated to proteins. Later, the sequence was submitted to NCBI and has got accession no. GU144796 with protein id ACZ36948. In BLASTN analysis, nucleotide test sequence showed 91% homology with D13914 (sequence from USA), whereas, protein test sequence was 75.9% homologous in BLASTP analysis with a number of protein sequences present in the database. The alignment score of test sequence with 67 other isolates of ZYMV retrieved from NCBI database was highest for USA among varied countries and lowest for China in case of nucleotides and Korea in case of proteins. Phylogenetic analysis revealed 91% similarity of the test virus sequence with a USA ZYMV CP (D13914) and 75.9% similarity of the partial polyprotein sequence with that of Japan (BAE75935). Conserved domain of the test virus was found to show homology with the potyvirus coat protein domain alignment collection (pfam00767). Computational restriction digestion revealed that 22 different restriction enzymes restrict present isolate of ZYMV. Secondary structures for polyprotein of the test virus was predicted which inferred dominance of alpha (􀄮) helix in the protein sequence.