BIOSYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF IRON NANOPARTICLES SYNTHESIZED BY Pseudomonas putida STRAIN LUA 15.1
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Date
2018-01
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UHF,NAUNI
Abstract
ABSTRACT
Nanotechnology is gaining tremendous incentive in the present century due to its capability of
modulating metals into their nanosize, which changes the chemical, physical and optical properties of metals.
The synthesis of nanostructured materials, especially metallic nanoparticles, has gained utmost interest over the
few decades owing to their unique properties that make them applicable in different fields of science and
technology. The biological method of nanoparticles synthesis is a relatively simple, cheap and environmentally
friendly than the conventional chemical and physical methods of synthesis. Aim of the present study was
procurement of Pseudomonas putida strain LUA15.1, conformation of the original strain using morphological,
biochemical and molecular techniques and its quantitative evaluation for iron nanoparticles synthesis. Different
physicochemical parameters were optimized for synthesis of iron nanoparticles by Pseudomonas putida strain
LUA15.1 using one variable at a time. Maximum iron nanoparticles synthesis was achieved at 35OC, pH: 8.0
and after 24 hrs of incubation with 5.0g/l peptone, 3.0g/l beef extract, 3 % inoculum size and 2.0mM ferrous
sulfate as substrate. In vitro synthesis of iron nanoparticles was carried and biosynthesized iron nanoparticles
were characterized using various spectroscopic and microscopic techniques UV-vis spectroscopy exhibited a
characteristic peak at 350nm, FTIR spectrum revealed presence of of alchol-phenol, alkenes, aromatic, alkane,
alkyl halide, amine, and ether biomolecules. XRD results confirmed that these nanoparticles were originally iron
nanoparticles. Scanning electron microscope showed that iron nanoparticles were both monodispersed in
aggregates with varation in shape triangular, square rectangular and rod, whereas shape and DLS graph revealed
average diameter of iron nanoparticles was 75nm. Antibacterial activity of biosynthesized iron nanoparticles
was assessed against five pathogenic bacteria, Agrobacterium tumefaciens, Pectobacterium carotovorum,
Xanthomonas axonopodis, Ralstonia solanacearum and Escherichia coli under in vitro conditions and
maximum zone of inhibition of 1.7 cm was observed for Agrobacterium tumefaciens. Pseudomonas putida
strain LUA15.1 and its extracellularly synthesized iron nanoparticles showed significant potential for
detoxification of four textile dyes viz., methylene blue, congo red, malachite green and brilliant green maximum
decolorization was observed for 20 ppm methylene blue dye with 99.1% decolorization. Phytotoxity effect of
methylene blue dye on five agriculturally important crops viz., Allium cepa, Brassica rapa, Solanum
lycopersicum, Spinacia oleracea and Vigna radiate was investigated and it has been observed that maximum
germination of 92%, maximum shoot length of 7.7cm and maximum root length 5.6 cm were in Spinacia
oleracea, Allium cepa and Solanum lycopersicum respectively with ironnanoparticle treated dye.
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