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Dr. Y. S. Parmar University of Horticulture & Forestry, Solan

Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, was established on 1st December, 1985 with the objective to promote education, research and extension education in the fields of Horticulture, Forestry and allied disciplines. Late Dr. Yashwant Singh Parmar, the first Chief Minister and the architect of Himachal Pradesh perceived the importance of Horticulture and Forestry to develop and improve the State economy which led to the establishment of this University. Its history lies in erstwhile Himachal Agricultural College, Solan, established in 1962 and affiliated to the Panjab University. It became one of the campuses of Agriculture Complex of Himachal Pradesh University on its formation in 1970. Consequent upon the establishment of Himachal Pradesh Krishi Vishvavidyalaya in 1978, this campus became its Horticulture Complex and finally in 1985, assumed the status of a State University, being the only University in the country engaged exclusively in teaching, research and extension in Horticulture and Forestry. The University is located at Nauni in Solan District of Himachal Pradesh, 13 km from Solan on Solan-Rajgarh Road, at an elevation of 1300 metres above mean sea level. Solan town is situated on national highway (NH-22) and is well connected by train and bus services. The University has four constituent colleges, out of which, two are located at the main campus Nauni, one for horticulture and the other for forestry, having 9 and 7 departments, respectively. The third College i.e., College of Horticulture & Forestry is located at Neri in Hamirpur District on Nadaun-Hamirpur state highway, about 6 Km from Hamirpur town and is well connected with bus service. The college offers three Undergraduate Degree Programmes i.e. BSc (Hons.) Horticulture, BSc (Hons.) Forestry and B. Tech. Biotechnology and MSc degree programme in a few subjects. The fourth college i.e. College of Horticulture and Forestry, Thunag (Mandi) is located at Thunag District Mandi. This college offer BSc (Hons.) Horticulture and BSc (Hons.) Forestry degree programme. In addition, there are five Regional Research Stations, 12 Satellite Stations and five Krishi Vigyan Kendras (KVKs) situated in different zones of the State.

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Subject: Biochemistry × Start date: 1994 ×
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Now showing 1 - 9 of 11
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    ThesisItemOpen Access
    STUDIES ON TANNASE OF ASPERGILLUS NIGER VAN TIEGHEM
    (CHAUDHARY SARWAN KUMAR HIMACHAL PRADESH KRISHI VISHVAVIDYALAYA ; PALMPUR, 2001) BHARDWAJ, RITA; BHAT, T. K
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    ThesisItemOpen Access
    STUDIES ON PARTIAL MOLECULAR CHARACTERIZATION OFDendrocalamus ha miltonii Munro.(Maggar Bambo.o)
    (DR. YASHWANT SINGH PARMAR UNIVERSITY OF HORTICULTURE AND FORESTRY ; SOLAN, 1999) SHARMA, ASHWANIKUMAR; KANWAR, KAMLESH
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    ThesisItemOpen Access
    Studies on in vitro cultured protoplasts of Robinia pseudoacacia L
    (DR. YASHWANT SINGH PARMAR UNIVERSITY OF HORTICULTURE AND FORESTRY ; SOLAN, 1999) BHARDWAJ, ANJU; KANWAR, KAMLESH
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    ThesisItemOpen Access
    IN VITRO SELECTION OF CARNATION PLANTS FOR RESISTANCE AGAINST FUSARIUM OXYSPORUM F.SP DIANTHI
    (COLLEGE OF HORTICULTURE DR. YASHWANT SINGH PARMAR UNIVERSITY OF HORTICULTURE AND FORESTRY, 1999) THAKUR, MANISHA; SHARMA, D. R
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    ThesisItemOpen Access
    CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA AND EVALUATION OF THEIR BIOCONTROL POTENTIAL AGAINST TOMATO BACTERIAL CANKER
    (UHF,NAUNI, 2014) GAUTAM, SWATI; SHIRKOT, C.K.
    ABSTRACT Bacterial canker caused by Clavibacter michiganensis subsp. michiganensis is known to cause significant economic losses to tomato production worldwide. Biological control has been proposed as an alternative to current chemical containment methods, which are often inefficient and may leave adverse effects on the environment. However, only little headway has so far been made in developing biocontrol strategies against C. michiganensis subsp. michiganensis. To address this knowledge gap, we investigated the antagonistic capacity of different PGPRs having multifarious plant growth promoting traits (P-solubilization, IAA production, siderophore production, lytic enzyme activity, and ability to fix atmospheric nitrogen) on C. michiganensis under in vitro and in planta conditions. PGPRs are highly diverse and in this study we focus on rhizobacteria as biocontrol agents. Their effects can occur via local antagonism to soil-borne pathogens or by induction of systemic resistance against pathogens throughout the entire plant. Several substances produced by antagonistic rhizobacteria have been related to pathogen controland indirect promotion of growth in many plants, such as siderophores, lytic enzymes and antibiotics. Induced systemic resistance (ISR) in plants resembles pathogen-induced systemic acquired resistance (SAR) under conditions where the inducing bacteria and the challenging pathogen remain spatially separated. Five antagonistic bacterial isolates were tested for biocontrol potential against C. michiganensis under net house conditions. Isolate S1 showed maximum reduction in disease incidence and disease index along with maximum increase in plant growth parameters. The isolate was identified as Bacillus amyloliquefaciensby 16S rDNA sequencing. Therefore, strain S1 had considerable biocontrol potential as well as plant growth promoting ability and could be used as a potential biocontrol agent against bacterial canker of tomato.
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    ThesisItemOpen Access
    EXPLORATION OF MICROBIAL DIVERSITY OF HIMACHAL PRADESH FOR PRODUCTION AND OPTIMIZATION OF LIPASE AND ITS APPLICATION
    (UHF,NAUNI, 2016) SHARMA, PUSHPINDER; SHARMA, NIVEDITA
    ABSTRACT Enzymes are considered as nature’s catalysts. Lipases are a class of enzymes which catalyze the hydrolysis of long chain triglycerides into polar lipids. Oil contaminated sites are highly probable source of lipogenic microorganisms and was utilized as a source for isolation of lipase producing microorganisms. In total, 50 lipoltytic bacteria have been screened from oil contaminated sites of Himachal Pradesh. Among them hyper lipolytic strains PS3 and PS4 were selected and identified as Bacillus methylotrophicus PS3 KX758632 and Brevibacterium halotolerans PS4 KX671556. Cultural conditions and process parameters viz. media types, pH, temperature, inoculum size, incubation time, substrate concentration, divalent ions and surfactants etc. were optimized firstly through classical one variable at a time (OVAT) followed by statistical optimization by employing central composite design of response surface methodology. The enzymes obtained from both the strains were purified to homogeneity by following a sequential purification approach. B. methylotrophicus PS3 lipase was purified to a final purification fold of 2.90 and had a molecular weight of  31.5 kDa whereas B. halotolerans PS4 lipase was purified to a final purification fold of 2.94 and had a molecular weight of  80 kDa. Lipase activity was found to be maximum at 40oC and pH 7.0 for both the strains. Applicability of purified lipase from both the strains was assessed by utilizing it for the removal of stains from the clothes. Both these strains along with branded detergents had significantly improve the cleansing of grease, butter, vegetable oil, olive oil and grease (white) within 30min as compared to the control. The high efficiency of lipase in enhancing the washing quality of detergents by destaining makes them a potential candidate for use in detergent industry.
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    ThesisItemOpen Access
    EFFECT OF PLANT GROWTH PROMOTING RHIZOBACTERIA, ARBUSCULAR MYCORRHIZA AND DIFFERENT LEVEL OF CHEMICAL FERTILIZERS ON GROWTH AND YIELD OF SWEET CHERRY (Prunus avium)
    (UHF,NAUNI,SOLAN, 2016) KHOSLA, SAIJAL; KAUSHAL, RAJESH
    ABSTRACT The deterioration of soil health and environmental conditions due to indiscriminate use of synthetic inputs in modern agriculture has promoted the use of beneficial microorganisms to increase crop yields and maintain soil ecological balance. Therefore, the present investigations were carried out to isolate and characterize the efficient indigenous PGPR and AM fungal isolates and to study their conjoint effect along with chemical fertilizers on growth and yield of sweet cherry. A total of seven efficient bacterial isolates were screened for multifarious PGP traits, out of which one bacterial isolate KHA8 was selected, which solubilized 422.50 μg/ml of insoluble phosphorous, showed 72.41% siderophore efficiency and inhibited selected fungi i.e. Rhizoctonia solani (30%), Fusarium oxysporum (53.24%) and Pythium aphanidermatum (32.33%). On the basis of morphological, biochemical characterization, the isolate was identified as Bacillus spp. The AMF spore population varied from 120.58 to 243.50 spores/50 g of soil and root colonization ranged from 58.20 to 80.50 per cent under natural conditions. The AM fungal species isolated from different aspect/ altitude/ location followed the distribution pattern of Glomus> Acaulospora> Gigaspora and Scutellospora with maximum (80%) frequency of occurrence for Glomus genus in cherry plantation. Multiplication of AM fungal spores was carried out with Pannicum maximum where root colonization was about 75% with spore count of 322.67 spores/50 g of soil after six months of mass multiplication. The application of consortium (PS1 and PS7) and indigenous AMF isolate (Glomus fasciculatum) at 90 per cent dose of NPK registered a significant increase in annual shoot extension (185.23 cm), leaf area (372.27 cm2), available N (413.95), P (86.70) and K (472.50) kg/hac over uninoculated control.
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    ThesisItemOpen Access
    Preliminary characterization of fluorescent Pseudomonas diversity producing plant growth regulators in apple and pear
    (2010) KAPOOR, RITIKA; KAUR, MOHINDER
    ABSTRACT Under the present study, an attempt was made to study production of bacterial plant growth regulators viz. auxins, gibberellins and cytokinins produced by indigenous strains of Pseudomonas species isolated from the rhizosphere of apple and pear. They all were screened out for the production of different plant growth promoting activities like antifungal, siderophores, phosphate solubilization, HCN, ammonia and protease. Four strains each of Pseudomonas species (PN-4-SAN, PN-10-SAN, AN-2-NAG, AN-4-NAG ) were selected for further studies with the aim to find out best indigenous plant growth regulators producing bacterial strains for application in field in order to increase yield and crop productivity and also to find out industrially important strains for production of plant growth regulators viz. auxins, gibberellins and cytokinins. The selected Pseudomonas isolates preferred nutrient broth for the growth and production of all three growth regulators. All the four strains produced maximum plant growth regulators at 72 h incubation period at pH 7.0 under shake condition while the optimum temperature for production of growth regulators was 28oC. An attempt was also made to extract, purify and evaluate plant growth regulators by thin layer chromatography, by spectrophotometric and specific bioassay method. By TLC, auxins gave pink spot gibberellins gave grey spot and cytokinins gave brown spot upon spraying with Salper reagent, solvent water: sulphuric acid and iodine respectively. Specific bioassays viz. Avena coleoptile straight test, α-amylase release test and radish cotyledon bioassays showed increased length, enzyme activity and increase in weight of cotyledons.
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    ThesisItemOpen Access
    EFFICIENCY OF PLANT GROWTH PROMOTING RHIZOBACTERIA FOR THE ENHANCEMENT OF TOMATO GROWTH
    (2012) SHARMA, RASHMI; SHIRKOT, C.K.
    ABSTRACT Plant growth promoting rhozobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. Environmental protection and the need to enhance agricultural output have made research in new sustainable technologies necessary. As in most sectors of agriculture, there is increasing demand for organic tomatoes. Therefore, the overall strategy for increasing crop yields, sustaining them at a high level and maintaining proper soil health must include an integrate approach to develop effective and more consistent active plant growyh promoting rhozobacteria (PGPR) inocula, because, there is a considerable experimental support for the idea that PGPR may be used as biofertilizers and biocontrol agent to increase the early growth of tomato seedlings in the nursery and subsequently the yield with concomitant decrease in the chemical fertilizers and pesticides. Therefore, the aim of this study is to screen the indegeneous plant growth promoting rhizobacterial isolates for multifarious plant growth promoting traits and to determine the effect of inoculation with PGPR’s on growth of tomato seedlings in filter paper assay and in soil less medium under growth chamber. The bacterial strains were characterized for plant growth promoting activities and antifungal-antibiotic activities under in vitro conditions. Seed bacterization with bacterial strains increased the germination percent and overall root/shoot parameters of tomato seedlings in filter paper assay and under growth chamber. Among twenty seven bacterial strains, including refrence strain, isolate S2 showed significant effect in filter paper assay with maximum increase in per cent germination, root length, plant dry weight and vigour index. Isolate *S25 showed maximum increase in root length, root dry weight, shoot dry weight and plant biomass in growth chamber. *S25 also possessed maximum P-solubilization, maximum IAA production, siderophore synthesis, chitinase activity, protease activity, ability to fix atmospheric nitrogen and antifungal activity against Alternaria solani, D.necatrix and Sclerotium rolfsii. Therefore, *S25 strain had considerable growth promotion and biocontrol ability, and it could be used as a biofertilizer or biocontrol agent.