CHARACTERIZATION OF PLANT GROWTH PROMOTING RHIZOBACTERIA AND EVALUATION OF THEIR BIOCONTROL POTENTIAL AGAINST TOMATO BACTERIAL CANKER
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Date
2014
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UHF,NAUNI
Abstract
ABSTRACT
Bacterial canker caused by Clavibacter michiganensis subsp. michiganensis is known
to cause significant economic losses to tomato production worldwide. Biological control has
been proposed as an alternative to current chemical containment methods, which are often
inefficient and may leave adverse effects on the environment. However, only little headway
has so far been made in developing biocontrol strategies against C. michiganensis subsp.
michiganensis. To address this knowledge gap, we investigated the antagonistic capacity of
different PGPRs having multifarious plant growth promoting traits (P-solubilization, IAA
production, siderophore production, lytic enzyme activity, and ability to fix atmospheric
nitrogen) on C. michiganensis under in vitro and in planta conditions. PGPRs are highly
diverse and in this study we focus on rhizobacteria as biocontrol agents. Their effects can
occur via local antagonism to soil-borne pathogens or by induction of systemic resistance
against pathogens throughout the entire plant. Several substances produced by antagonistic
rhizobacteria have been related to pathogen controland indirect promotion of growth in many
plants, such as siderophores, lytic enzymes and antibiotics. Induced systemic resistance (ISR)
in plants resembles pathogen-induced systemic acquired resistance (SAR) under conditions
where the inducing bacteria and the challenging pathogen remain spatially separated. Five
antagonistic bacterial isolates were tested for biocontrol potential against C. michiganensis
under net house conditions. Isolate S1 showed maximum reduction in disease incidence and
disease index along with maximum increase in plant growth parameters. The isolate was
identified as Bacillus amyloliquefaciensby 16S rDNA sequencing. Therefore, strain S1 had
considerable biocontrol potential as well as plant growth promoting ability and could be used
as a potential biocontrol agent against bacterial canker of tomato.
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