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Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola
Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola was established on 20th October, 1969 with its head-quarter at Akola. This Agricultural University was named after the illustrious son of Vidarbha Dr. Panjabrao (alias Bhausaheb) Deshmukh, who was the Minister for Agriculture,Govt. of India. The jurisdiction of this university is spread over the eleven districts of Vidarbha. According to the University Act 1983 (of the Government of Maharashtra), the University is entrusted with the responsibility of agricultural education, research and extension education alongwith breeder and foundation seed programme.
The University has its main campus at Akola. The instructional programmes at main campus are spread over in 5 Colleges namely, College of Agriculture, College of Agricultural Engineering & Technology, College of Forestry, College of Horticulture and Post Graduate Institute. At this campus 4 degree programmes namely B.Sc.(Agri.) B.Sc. (Hort.), B.Sc. (Forestry) and B.Tech. (Ag. Engg.) , two Master’s Degree Programmes viz. M.Sc.(Agri.) and M.Tech. (Agri.Engg.) and Doctoral Degree Programmes in the faculties of Agriculture and Agril. Engineering are offered.
The University has its sub-campus at Nagpur with constituent College, College of Agriculture which offers B.Sc.(Agri.) and M.Sc.(Agri.) degree programmes. The Nagpur Campus is accomplished with a garden, surrounded by its natural beauty and a well established Zoo which attract the general public and visitors to the city. A separate botanic Garden is being maintained on 22 hectares with a green house for the benefit of research workers.
In addition there are 2 affiliated grant-in-aid colleges and 14 private non-grant-in-aid colleges under the umbrella of this University
A Central Research Station is situated at the main Campus which caters to the need of research projects undertaken by Crop Scientists of the principle crops of the region are Cotton, Sorghum, Oilseeds and Pulses.
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ThesisItem Open Access NANO ENCAPSULATION OF dsRNA OF METAMORPHOSIS RELATED GENE AND ITS INSECTICIDAL POTENTIAL AGAINST DIAMOND BACK MOTH.(Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2022-03-31) PIMPALZARE, PRANALI SUBHASHRAO.; Deshmukh, Mr. A. G.The present investigation entitled “Nano encapsulation of dsRNA of metamorphosis related gene and its insecticidal potential against diamond back moth” was carried out at Biotechnology Centre, Department of Agricultural Botany, Post Graduate Institute, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during the academic year 2019-2021. RNA interference (RNAi) technology post transcriptional mechanism triggerd by dsRNA to silence specific genes by down regulating their expression. It has become a potential strategy for functional and regulatory studies of insect genes and has potential to control insect pest. Though it has been challenging to produce effective RNAi in lepidopteran Diamond back moth (P. xylostella). Some important factors significantly influencing the silencing potential effects are concentration and length of dsRNA, nucleotide sequence, and determination of the silencing effect and life stage of the target organism. The P.xylostella shows RNAi effects after feeding of nanoencapsulated dsRNA. Considering the potential impact on the normal physiological functions of healthy adults, Juvenile Hormone Esterase gene select as targets for dsRNA mediated gene silencing. Juvenile Hormone Esterase gene sequence obtained from NCBI database. Off targets were predicted to avoid non target effects. Possible RNAi sites were depicted to identify the region having maximum RNAi targets. The aim of studies was to prepared PLGA nanoparticles, because of their possibility to target specific cells with high biodegradability and biocompatibility. It is approved for human use by the Food and Drug Administration. Here, we describes in great details about the formation and characterization of PLGA nanoparticles. Poly (lactic-co-glycolic acid) nanoparticles synthesized using double emulsification method. PVA (poly vinyl alcohol) used as stabilizer during the synthesis of PLGA nanoparticles. This protocol can be readily adapted to use alternative emulsifiers PVA (e.g. Poly vinyl alcohol) or solvent (e.g. methanol). A typical peak at 380nm having 0.56 absorbance on UV- visible spectroscopy indicates synthesis of PLGA nanoparticles. Particle size and zeta potential are determined with Nanoparticle size analyzer and Zeta potential analyzer. The PLGA nanoparticles characterized during present study showed 244.9 nm particle sizes and -4.59 mV zeta potential. FTIR analysis, carried out in wave range 1000- 4000 cm-1, showed different functional groups like 3884 cm-1 and 3410 cm-1 (Alcohol), 1780 cm-1 (Carbonyl group), 1494 cm-1 and 1666 cm-1 (Glycolic acid) 1382 cm-1, and 1187 cm-1 (Esters), 1472 cm-1 and 1096 cm-1(Vinyl) in PLGA NPs synthesis. The process of encapsulation of dsRNA with PLGA NPs done to increase the insecticidal potential of dsRNA-JHE(A) and dsRNA-JHE(B). The encapsulated nanoparticles were also characterized by UV spectrophotometry, particle size was determined with Nanoparticle size analyzer and Zeta potential analyzer. We provide representative images for nanoparticles produced. Effect of dsRNA and encapsulated dsRNA with PLGA NPs was checked out by performing insect bioassay studies on P. xylostella larvae. The dsRNAs and encapsulated dsRNA with PLGANPs were individually spread on to the cabbage leaf discs and twelve larvae were released into 3 replication. We detected that encapsulated dsRNA-JHE(A) and encapsulated dsRNA-JHE(B) which had the highest mortality 60% and 72% as compared to control 0% (only water) and dsRNA 36% JHE(A) and 48% JHE(B). These findings largely broaden the target selection for RNAi-based technology and deliver dsRNA into the insect and potential against insect damage and insect control management.ThesisItem Open Access Title : EVALUATION OF NSE AT DIFFERENT CONCENTRATIONS AND SOAKING PERIODS AGAINST MAJOR PESTS OF TOMATO.(Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2022-10-05) Authors : NAKADE, NIKESH UDARAM.; Advisor : Yadgirwar, Dr. P. V.Abstract : The present investigation entitled “EVALUATION OF NSE AT DIFFERENT CONCENTRATIONS AND SOAKING PERIODS AGAINST MAJOR PESTS OF TOMATO” was undertaken on tomato crop (Variety local popular hybrid Abhilash) under field condition At Post Ambadi, Tq. Kuhi, Dist. Nagpur during kharif season of 2020-21 with a view to study effect of different soaking periods on field efficacy of NSE against major pests of tomato. Similarly, investigations were also aimed to evaluate field efficacy of different concentrations of NSE and work out economics of different treatments. The experiment was laid out in a randomized block design with seven treatments replicated three times. The treatments comprised of NSE 5% with 12, 24 and 36 hours soaking period, NSE 7% with 12, 24 and 36 hours soaking period and untreated control. The defoliator (Spodoptera litura), whitefly (Bemisia tabaci), aphid (Myzus persicae), leaf miner (Liriomyza trifolii) and fruit borer (Helicoverpa armigera) were observed as major pests causing damage at various growth stages of the crop. Among the different treatments NSE 7% with 12 hours soaking period was found significantly most effective and was at par with NSE 5% with 12 hours soaking period against all major pests viz., Spodoptera litura, whitefly, aphid, leaf miner and fruit borer. The next effective treatments were, NSE 7% with 24 hours soaking period, NSE 5% with 24 hours soaking period, NSE 7% with 36 hours soaking period and NSE 5% with 36 hours soaking period . However, significantly highest infestations was observed in control (water spray). The highest yield was recorded in NSE 7% with 12 hours soaking period (243.38 q/ha) which was at par with NSE 5% with 12 hours soaking period (229.27 q/ha) and NSE 7% with 24 hours soaking period (218.69 q/ha). Those treatments were followed by NSE 5% with 24 hours soaking period (208.14 q/ha), NSE 7% with 36 hours soaking period (204.58 q/ha) and NSE 5% with 36 hours soaking period (197.53 q/ha). The lowest yield was recorded in untreated control (172.83 q/ha). The highest incremental cost benefit ratio was obtained in the treatment of NSE 7% with 12 hours soaking period (1:6.39) followed by NSE 5% with 12 hours soaking period (1:5.60), NSE 7% when soaked for 24 hours (1:3.80), NSE 5% with 24 hours soaking period (1:3.13), NSE 7% with 36 hours soaking period (1:2.32) and NSE 5% with 36 hours soaking period (1:1.89), respectively.ThesisItem Open Access Title : EXPLOITATION OF NANOPARTICLES FOR SEED TREATMENT IN CHICKPEA.(Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2022-09-20) Authors : DHAKADE, PRATIK PRAMODRAO.; Advisor : Akhare, Dr. A. A.Abstract : The present investigation entitled “Exploitation of nanoparticles for seed treatment in chickpea” was carried out in Seed Technology and Research unit at Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola in the academic year 2020-21. The study was aimed to evaluate the effect of green synthesized nanoparticles on seed growth parameters like seed germination, seed vigor, and seed health, and also see the effect on various yield attributes of chickpea. Concentrations of 1000, 750, 500, 250 ppm were made to perform seed treatment. 250 ppm shows a better effect on germination percentage, seed vigor index I and II and seed health than control during the period of seed germination. In number of pods per plant T6 (75% RDF (100% N/K with 75% P) + seed coating of nano Zn + Fe (Zinc + Iron) @ 125 ml ha-1) shows the better result (175.1 pods per plant) than control. In seed yield per plant T10 (75 % RDF (100 % NPK with 75 % Zn/Fe) + Seed coating of nano Zn+Fe (Zinc + Iron) @ 62.5 ml ha-1+ Foliar spray of nano Zn + Fe (Zinc + Iron) @ 250 ml ha-1) shows the better result (46 g seed yield per plant) than control. In seed yield per plot T5 (75 % RDF (100 % N/K with 75 % P) + Seed coating of nano P (Phosphorous) @125 ml ha-1) shows the better result (5.45 kg seed yield per plot) than control. After post-harvest T9 (75 % RDF (100 % N/K with 75 % P) + Seed coating of nano P (Phosphorous) @ 62.5 ml ha-1+ Foliar spray of nano P (Phosphorous) @ 250 ml ha-1) shows the better result ( 94 germination %) in seed vigor index I T6 (75 % RDF ( 100 % NPK with 75 % Zn/Fe) + Seed coating of nano Zn + Fe (Zinc + Iron) @125 ml ha-1) shows the better result (2984) than control and in seed vigor index II T8 (100 % RDF + Seed coating of nano Zn + Fe (Zinc + Iron) @ 62.5 ml ha-1+ Foliar spray of nano Zn + Fe (Zinc + Iron) @ 250 ml ha-1) shows the better result (62.3) than control. Fe-NP, ZnO-NP, and P-NPs were synthesized using Datura inoxia, Aloe vera, and Aspergillus tubingensis respectively. Nanoparticles were confirmed by UV-Visible photo spectrometer, Particle size analysis, Zeta potential, and Fourier transform infrared spectroscopy. Fe-NPs show the optimum peak at 270 nm, ZnO-NPs show the optimum peak at 360 nm and P-NPs show the optimum peak at 280 nm. Fe-NPs having an average size of 29.77 nm and ZnO-NPs having an average size of 37.8 nm were observed by Particle size analyzer. Fe-NPs having a zeta potential of -22.9 mV and ZnO-NPs having a zeta potential of -14.8 mV were observed by Zeta potential analysis.ThesisItem Open Access Title: IN-VITRO PROPAGATION OF JAMUN (Syzygium cumini L.).(Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2022-10-26) Authors: BOCHARE, PRACHI RAJENDRA.; Advisor: Rathod, Dr. D. D.Abstract: The present study was carried out with the aim to study an in-vitro propagation of Jamun (Syzygium cumini L.) species using local species using Lloyd and McCown woody plant medium (WPM) in CRD experimental design at Biotech Centre, Dr. PDKV, Akola during 2019-20 and 20-21. Effect of WPM with little modification of cytokines, auxins, and plant growth-stimulating hormones on different growth parameters on in-vitro propagation were optimized. Surface sterilized local jamun nodal explant using HgCl2, the combination of antibiotics and antioxidants were attempted. Surface sterilization of the explants was effectively achieved using mercuric chloride at 0.1% (4 minutes) in combination with 1% streptomycin as it recorded lower contamination (20%) and higher survival (76.66%). Surface sterilized nodal explant using HgCl2, the combination of antibiotics and antioxidants were attempted using WPM with BAP (6-Benzylamino purine), Kn (kinetin) shown good results. Nodal explants were cultured on WPM supplemented with BAP (6-Benzyl amino purine 8.0 and 9.0 µM/l), Kn (kinetin 8, 8.5 and 9 µM/l). In which, WPM with BAP + Kn @ 8 µM/l was proved to be significantly superior at 1% level for high survival rate after 3rd week 76.66% and requires less time 25.59 days to shoot sprout. Whereas BAP + Kn @ 8.0 + 8.5 µM/l was proved best with the highest 2.97 shoot multiples. Shoot proliferation and multiplication were performed on WPM supplemented with BAP @ 13 and 14 µM/l, Kinetin @ 8.0, 8.5, and 9.0 µM/l, and NAA (Naphthalene acetic acid) @ 5 µM/l. The treatment combination of WPM with BAP + Kn + NAA @ 13+8.5+5.0 µM/l were proved to be significantly superior at 1% for high frequency multiple shoot induction. The response was shown a maximum of 5.66 shoot multiples after 2nd subculture, followed by 3.94 shoot multiples after 1st subculture with significantly highest 1.98cm shoot length elongation upon WPM with 13.0+ 8.5+5.0 µM/l BAP + Kn + NAA medium as against 0.57cm of control (WPM without growth regulators). Therefore, woody plant media with above modification found promising for in-vitro propagation of Jamun species.ThesisItem Open Access Title : CHARACTERIZATION OF ECONOMICALLY IMPORTANT BAMBOO SPECIES USING MORPHOLOGICAL AND MOLECULAR MARKERS.(Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2022-07-09) Authors : PIPRADE, DHANASHRI VIJAYRAO.; Advisor : Rathod, Dr. D. R.Abstract : Present study entitled “Characterization of economically important bamboo species using morphological and molecular markers” was attempted at Biotechnology Centre, Dr. PDKV, Akola during 2020-21 and 2021-22. Main aim was to characterize economically important bamboo species viz., Dendrocalamus stocksii, Bamboosa balcoa, Dendrocalamus strictus, Bamboosa tulda, Bamboosa vulgaris. The morphological characterizations of these important bamboo species were attempted based on field-based observations. This was carried out by samples collected randomly from three plants of each species in field grown Bamboo Sectum, College of Agriculture, Dr. PDKV, Nagpur campus for 21 traits includes, 17 quantitative and 4 qualitative traits. B. tulda has found highest internodal distance 45.41cm, followed by D. stocksii (41.39cm) and the lowest D. strictus (30.78 cm). In case to yield, B. balcoa was found highest with 41.49 t/ha, followed by D. stocksii (35.22 t/ha), B. tulda (28.39 t/ha), B. vulgaris (17.48 t/ha) and lowest in D. strictus 12.53 t/ha, respectively. While culm height and number of culms/clumps sequentially B. vulgaris, D. strictus, B. tulda, D. stocksii and B. balcoa found 17.33m, 10.36m, 10.33m, 10.27m and 8.27m and B. tulda, D. strictus, D. stocksii, B. vulgaris and B. balcoa with 54.19, 45.22, 237.33, 26.03 and 9.51 respectively. Sp1 and sp5 were non-thorny while others are thorny natures and all spp. are economically important. Species genetic diversity was assessed using five polymorphic SSRs in all the spp. Dendrogram was constructed using Jaccard’s similarity coefficient and genotypes were grouped into three clusters based on SSR profile. A total of five SSR markers (RM-154, RM-224, RM-226, RM-338 and RM-340 were used for molecular genotyping of five important bamboo spp. Out of which, RM-224 and RM- 340 shows highest polymorphic alleles (3.0) each, followed by RM-226 (2.80), RM-154 (2.0) and least in RM-338 (1.38) Among bamboo spp., highest allelic diversity observed in D. stocksii, followed by B. tulda (13.00) and lowest in B. balcoa and vulgaris (11.00). Whereas, statistical analysis indicates that B. balcoa and B. tulda possess more genetic diversity with 47.51% and 43.85 % while B. vulgaris has lowest diverse (20.33%). Based on dendrogram, it proves that D. stocksii and D. strictus are closely related and shows highest similarity among them. While bamboo species, B. tulda and vulgaries shows closest spp. and they are genetically distance with B. balcoa. The molecular characterizing by using five SSR makers will be helpful for bamboo nursery persons for species identification and characterization of economically important bamboo species using SSR markers. The morphological and molecular markers represent similarity and distance between the bamboo spp. taken under study. The information generated will be useful for future Bamboo breeding programme.ThesisItem Open Access Title : EFFECT OF MODIFIED WOODY PLANT MEDIA ON SHOOT PROLIFERATION OF COMMERCIAL BAMBOO (Bambusa tulda L.).(Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2022-07-27) Authors : POTE, CHAITALI MAROTI.; Advisor : Rathod, Dr. D. R.Abstract : Present investigation entitled “Effect of modified woody plant media on shoot proliferation of commercial bamboo (Bambusa tulda L.)” was conducted at Biotechnology Centre, Dr PDKV, Akola during year 2019-20 and 2020-21 with the aim to study efficiency and effectiveness of modified woody plant media on culture establishments of commercial species of bamboo and optimization of combination of woody plant media for in-vitro shoot proliferation. The mother explants source were obtained from the well-established bamboo setum developed under AICRP on Agroforestry, College of Agriculture, Dr. PDKV, Nagpur. The nodal explants of B. tulda spp. having 3-4-year-old were taken and treatment with 0.1% tween-20 for 20 mins, 1% carbendazim for 30 mins, 1% streptomycin for 20 mins and 0.1% HgCl2 for 10 mins. These were inoculated in the month of Jan-Feb and shown maximum initiation rate 92.47% with lowest (20.88%) contamination percentage. However, explants of size 3cm showed effective growth with high multiplication rate (71.79%). The nodal explants when cultured on WPM media supplemented with 3 mg/l BAP (6-Benzylamino purine) showed high initiation i.e., 81.41% and survival rate of 45.93%. Shoot multiplication and proliferation was performed on WPM media supplemented with 3 mg/l BAP+2 mg/l kinetin +0.5 mg/l IBA was found best at 1% level of significance with highest multiplication and survival rate 60.99% and 69.88% respectively after 8th week of its inoculation while in same treatment the bud break occurred after 7th day of inoculation. Efficiency of WPM on various agar concentration was studied and it was recorded that 0.8% agar was more effective as compared with liquid media and agar at 0.3% and 0.6%. Various adjuvants and adsorbents namely; ascorbic acid, citric acid,1% charcoal, streptomycin was added in media composition to control browning which caused due to phenolic release. Among these, 1% charcoal when added to media supplemented with WPM+ 3 mg/l BAP +2 mg/l kinetin +0.5 mg/l IBA minimum browning rate to 13.33% and ascorbic acid at 50 mg/l +Citric acid at 25 mg/l when pretreated to explant showed highest initiation and survival rate 82.45 and 54.80 %, respectively. Micropropagation protocol developed will ensure multiplication of large number of B. tulda bamboo species.ThesisItem Open Access Title: IN-VITRO REGENERATION STUDIES OF AN ENDANGERED MEDICINAL PLANT SARPAGANDHA (Rauwolfia Serpentina L.).(Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2022-10-04) Authors: Wahurwagh, Arti Gopal.; Advisor: Deshmukh, Shri. Amit G.Abstract: The nodal segment and shoot tips were excised from a field grown plants for the investigation and inoculated on Murashige and Skoog medium supplemented with BAP. Nodal segment proved best as explant for in-vitro shoot proliferation than shoot tips. Therefore, nodal segment was used as an explant in present investigation. Among all the treatments bavistin 0.1% for 30 min, streptomycin 1.0% for 15 min, sodium hypochlorite 1.0% for 10 min and mercuric chloride 0.1% for 10 min duration was recorded as the best for surface sterilization and gave higher survival of explants. MS media supplemented with BAP with various concentrations in which MS+ 5 mg/l showed early response in ±6.93 days, number of shoots found was 4.13, regeneration 76.33% and height 5.53 cm. MS media supplemented with combination of BAP and IBA proved more responsive and effective for proliferation than BAP alone. MS+ 5 mg/l BAP + 0.5 mg/l IBA was found significantly better with 5.52 number of shoots and 81.30% shoot proliferation. Explants were found slightly more responsive in media combination MS + 5 mg/l BAP + 1.0 mg/l IBA with ±6.53 days and maximum height of shoots with 6.93 cm. For in-vitro shoot multiplication 3% sucrose concentration in MS media was proved best. Root induction in the regenerated shoots was found best in ½ MS media supplemented with 2.5 mg/l NAA + 2.0 mg/l IAA + 0.7% agar + 3% sucrose+ 0.65 g/l charcoal it responded in ±29.3 days and showed the highest root length with 2.9 cm among any other treatments. 1:3 soilrite and cocopeat hardening media with watering after 1 day proved best for primary hardening of the plantlets with maximum survival. The results obtained from the present investigation indicate that, in-vitro regeneration protocol will provide technique that can be successfully used for mass in-vitro propagation of sarpagandha species, Rauwolfia serpentina L.ThesisItem Open Access Title : Differential gene expression profiling during Fusarium wilting in chickpea using cDNA SCoT markers.(Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2022-07-20) Authors : SHARMA, PALAK.; Advisor : Moharil, Dr. M. P.Abstract : Chickpea (Cicer arietinum L.) is an annual, autonomous legume cultivated over 30 different countries having genome size of 740 Mbp and has a place within family Leguminoseae. India is the fundamental and prime producer of the chickpea contributing 65% in total world chickpea production and 75% of area coverage in the world. Various abiotic and biotic stresses are responsible for the loss in chickpea productivity. Fusarium wilt, caused by Fusarium oxysporum f. species ciceris is an economically important disease of chickpea. The pathogen can survive in soil for more than six years even in the absence of chickpea and causes 10%-15% yield losses annually. Keeping in view of importance of this crop and potential threat of Fusarium wilt in chickpea growing areas and to address the need for genomic tools in chickpea for Fusarium wilt resistance, the present investigation entitled “Differential Gene Expression Profiling During Fusarium Wilting in Chickpea Using cDNA SCoT Markers” was undertaken to determine differential gene expression analysis using cDNA-SCoT profiling to identify differentially expressed fragments (DEF’s) in resistant and susceptible cultivars. This study focused on which genes are responsible for Fusarium wilt resistance in the chickpea genotypes. This programme was carried out during 2019-21 at Biotechnology Centre, Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. Pathogenicity test of Fusarium oxysporum f.sp.ciceri by root dip inoculation method was performed which showed 84 percent wilting in susceptible cultivar at 25th days after inoculation whereas, in resistant cultivar few plants with wilting symptoms were observed. Characterization of resistant and susceptible genotypes of chickpea using quantified cDNA of both plant samples was profiled using 13 SCoT primers, all primers showed reproducibility. About 77 amplicons were generated amongst that 63 were polymorphic and 14 were monomorphic. The polymorphic amplicons were re-amplified (nested PCR) and sequenced using Applied Biosystem by Sanger sequencing method. The DEF’s were further analyzed using computational tools viz., BLAST homology, string protein interaction. Similarity search showed that both the differentially expressed fragment showed maximum homology by i.e 84.21% and 91.43% with Cicer arietinum cysteine rich PAK1 inhibitor like mRNA (LOC101498699) which act as a defensin like peptide. The P21 activated kinase 1 (PAK1) inhibitory compound having antifungal activity. Plant cysteine rich peptides were able to inhibit pathogenic growth and helps the legume formation in plants. String protein interaction studies revealed that the target protein interacts with the regulatory proteins like Receptor-like protein 12; Leucine-rich repeat receptor protein kinase EXS-like which are Leucine Rich Receptor Like Kinase contributes to Fusarium resistance in cereals. Cicer arietinum cystine rich receptor-like protein kinase (XP_004500476.1) interact with many other proteins which suggest the suitability of using this gene as a candidate gene in development of transgenic plants resistance against Fusarium wilt. The cDNA-SCoT technique is becoming increasingly important in the study of differentially expressed genes, the discovery of novel genes, and the exploration of molecular pathways. It should be made available to future researchers as an alternative to cDNA-AFLP, cDNA-SRAP, and other comparable technologies for multifunctional plant gene differential expression applications and studies. The information gained from identifying differentially expressed DEFs in tissues of infected and uninfected plants could be used to predict gene(s) involved in Fusarium wilt resistance in chickpea genotypes, for further, more detailed studies.ThesisItem Open Access Title : ASSESSMENT OF SILICON EFFECT IN RESPONSE TO CHARCOAL ROT IN SOYBEAN (Glycine max L.).(Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra., 2022-10-04) Authors : KHODE, VAIBHAVI.; Advisor : JADHAV, Dr. P. V.Abstract : The present study entitled “Assessment of silicon effect in response to charcoal rot in soybean (Glycine max L.)” was carried out at Department of Agricultural Botany, Dr. PDKV, Akola during the academic year 2020-21 to evaluate the effect of silicon in response to charcoal rot disease in soybean genotypes. Considering the severity of the disease, potassium silicate (K2SiO3) which is known for activating defense mechanism and enhancing the morphological characters in plants. To identify effect of K2SiO3, mass culture of M. phaseolina was prepared for in-vitro screening under sick pot condition for charcoal rot tolerance in soybean genotypes. Silicon gets accumulated in higher concentration in the epidermal cells and has been detected to benefit the plants that are exposed to biotic stresses. The susceptible genotype, TAMS-38 in charcoal rot infected condition showed disease symptoms whereas resistant genotype AMS-MB-5-18 showed normal growth. But in both the treatments 0.7mM and 1.7mM of K2SiO3 (K2SiO3 + C.R. infected), TAMS-38, AMS-MB-5-18 and JS-335 showed tolerance to charcoal disease as well as healthy growth. To identify the amount of accumulation of silicon in the susceptible and resistant genotypes, SEM-EDX analysis was done. The result revealed higher deposition of the silicon in the trichomes of the susceptible genotype. Accumulation of Si in the genotypes and plant response against charcoal rot disease with different morphological observations was recorded. To assess the in-vitro effect of silicon at phenotypic level various morphological characters were recorded such as plant height (cm), number of branches, 50% flowering (D), days to maturity (D), number of pods per plant, grain yield per plant (g) and 100 seed weight (g). The analysis of variance showed significant difference between the genotypes of different concentrations of Si treated pots (0.7mM and 1.7mM of K2SiO3) with charcoal rot, control pot and Si control. The phenotypic screening of soybean genotype against charcoal rot disease revealed that K2SiO3 leads to better adaptability in soybean genotype under charcoal rot disease condition. The effect of Si was also studied at molecular level by using cDNA-RAPD profiling which revealed due to K2SiO3 treatments various genes were activated in response to charcoal rot disease. Each cluster represents different response towards charcoal rot in each individual genotype. Out of the total screened alleles 75 were monomorphic alleles with an average of 3.75 and 69 were polymorphic alleles with an average of 3.45. Results showed an average of 47.68 polymorphism percent. The IDVs (intensity derived values) were calculated to study expression of varying size of polymorphic amplicons. The highest IDV (241002) value was recorded for primer OPH-12 in 1.7 mM of K2SiO3 treated plant of susceptible genotype TAMS-38. The study revealed that both the treatments 0.7mM and 1.7mM of K2SiO3 showed healthier growth in all three soybean genotypes. 1.7mM of K2SiO3 treatment showed superior results at morphological as well as molecular level.
