Title : EFFECT OF MODIFIED WOODY PLANT MEDIA ON SHOOT PROLIFERATION OF COMMERCIAL BAMBOO (Bambusa tulda L.).
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Date
2022-07-27
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Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
Abstract : Present investigation entitled “Effect of modified woody plant media on shoot proliferation of commercial bamboo (Bambusa tulda L.)” was conducted at Biotechnology Centre, Dr PDKV, Akola during year 2019-20 and 2020-21 with the aim to study efficiency and effectiveness of modified woody plant media on culture establishments of commercial species of bamboo and optimization of combination of woody plant media for in-vitro shoot proliferation. The mother explants source were obtained from the well-established bamboo setum developed under AICRP on Agroforestry, College of Agriculture, Dr. PDKV, Nagpur. The nodal explants of B. tulda spp. having 3-4-year-old were taken and treatment with 0.1% tween-20 for 20 mins, 1% carbendazim for 30 mins, 1% streptomycin for 20 mins and 0.1% HgCl2 for 10 mins. These were inoculated in the month of Jan-Feb and shown maximum initiation rate 92.47% with lowest (20.88%) contamination percentage. However, explants of size 3cm showed effective growth with high multiplication rate (71.79%). The nodal explants when cultured on WPM media supplemented with 3 mg/l BAP (6-Benzylamino purine) showed high initiation i.e., 81.41% and survival rate of 45.93%. Shoot multiplication and proliferation was performed on WPM media supplemented with 3 mg/l BAP+2 mg/l kinetin +0.5 mg/l IBA was found best at 1% level of significance with highest multiplication and survival rate 60.99% and 69.88% respectively after 8th week of its inoculation while in same treatment the bud break occurred after 7th day of inoculation. Efficiency of WPM on various agar concentration was studied and it was recorded that 0.8% agar was more effective as compared with liquid media and agar at 0.3% and 0.6%. Various adjuvants and adsorbents namely; ascorbic acid, citric acid,1% charcoal, streptomycin was added in media composition to control browning which caused due to phenolic release. Among these, 1% charcoal when added to media supplemented with WPM+ 3 mg/l BAP +2 mg/l kinetin +0.5 mg/l IBA minimum browning rate to 13.33% and ascorbic acid at 50 mg/l +Citric acid at 25 mg/l when pretreated to explant showed highest initiation and survival rate 82.45 and 54.80 %, respectively. Micropropagation protocol developed will ensure multiplication of large number of B. tulda bamboo species.
Description
Description : Bamboo (Bambusa tulda L.) are ever green and most demanding economical social forestry tree species. B. tulda has great commercial value due to its special characteristics of making straight sticks. It is widely used in incense stick making and the culm of B. tulda is also used for various other purposes including fencing, handicrafts, basketry, hut making and it also significantly contributes as raw materials in paper and pulp industry. Keeping in view of the vast unexploited potential use of B. tulda the large plantation is required however, propagation of bamboo through seeds is limited due to long flowering cycles, short seed viability and seed sterility. Vegetative propagation is inadequate to fulfill the demand of planting stock hence plant tissue culture offers special advantage, as all drawbacks can be overcome by using micropropagation techniques. Considering this aspect, the present study is proposed and performed in Plant tissue culture Laboratory, Center of excellence in Plant Biotechnology Dr. PDKV Akola. The efficiency and effectiveness of Woody plant media (WPM) is assessed with reference to B. tulda spp. for high shoot proliferation and multiplication. It will support to enhance the nursery activities to bring large area under bamboo cultivation of elite species like B. tulda L. as per domestic and industrial utility.
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Citation : POTE, CHAITALI MAROTI. (2021). Effect of modified woody plant media on shoot proliferation of commercial bamboo (Bambusa tulda L.). Biotechnology Centre, Department of Agricultural Botany. Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2021. Print. xiii, 95p. (Unpublished)