Title: IN-VITRO REGENERATION STUDIES OF AN ENDANGERED MEDICINAL PLANT SARPAGANDHA (Rauwolfia Serpentina L.).
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Date
2022-10-04
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Publisher : Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Maharashtra.
Abstract
Abstract: The nodal segment and shoot tips were excised from a field grown plants for the investigation and inoculated on Murashige and Skoog medium supplemented with BAP. Nodal segment proved best as explant for in-vitro shoot proliferation than shoot tips. Therefore, nodal segment was used as an explant in present investigation. Among all the treatments bavistin 0.1% for 30 min, streptomycin 1.0% for 15 min, sodium hypochlorite 1.0% for 10 min and mercuric chloride 0.1% for 10 min duration was recorded as the best for surface sterilization and gave higher survival of explants. MS media supplemented with BAP with various concentrations in which MS+ 5 mg/l showed early response in ±6.93 days, number of shoots found was 4.13, regeneration 76.33% and height 5.53 cm. MS media supplemented with combination of BAP and IBA proved more responsive and effective for proliferation than BAP alone. MS+ 5 mg/l BAP + 0.5 mg/l IBA was found significantly better with 5.52 number of shoots and 81.30% shoot proliferation. Explants were found slightly more responsive in media combination MS + 5 mg/l BAP + 1.0 mg/l IBA with ±6.53 days and maximum height of shoots with 6.93 cm. For in-vitro shoot multiplication 3% sucrose concentration in MS media was proved best. Root induction in the regenerated shoots was found best in ½ MS media supplemented with 2.5 mg/l NAA + 2.0 mg/l IAA + 0.7% agar + 3% sucrose+ 0.65 g/l charcoal it responded in ±29.3 days and showed the highest root length with 2.9 cm among any other treatments. 1:3 soilrite and cocopeat hardening media with watering after 1 day proved best for primary hardening of the plantlets with maximum survival. The results obtained from the present investigation indicate that, in-vitro regeneration protocol will provide technique that can be successfully used for mass in-vitro propagation of sarpagandha species, Rauwolfia serpentina L.
Description
Description: The present investigation was conducted at Plant Tissue Culture Laboratory, Biotechnology Centre, Dr.P.D.K.V., Akola (MH). Sarpagandha (Apocynaceae) is a woody perennial medicinal shrub, and the plant is rare, endangered plant. Present investigation showed micropropagation protocol for mass in-vitro propagation of the plant to fulfill the demand of pharmaceuticals and industries at commercial and economic level.
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Citation
Citation: Wahurwagh, Arti Gopal. (2021). In-vitro regeneration studies of an endangered medicinal plant sarpagandha (Rauwolfia serpentina L.). Biotechnology Centre, Department of Agricultural Botany, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola. M. Sc. 2021. Print. xii, 95p. (unpublished).