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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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20231
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Subject: Agricultural Biotechnology × Start date: 2023 ×
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Now showing 1 - 3 of 3
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    ThesisItemEmbargo
    DEVELOPMENT OF MYMV RESISTANCE IN GREENGRAM [Vigna radiata (L.) Wilczek] USING BULK SEGREGANT ANALYSIS
    (2023-01-20) POOJITHA; K. M. HARINIKUMAR
    Greengram (Vigna radiata (L). Wilczek) is an important pulse crop owing to its short growth duration, low water requirement, and its suitability for crop rotation and nutritional security being a rich source of protein. Greengram’s yield is greatly affected by numerous abiotic and biotic factors. Biotic stress produced by viruses especially Mungbean Yellow Mosaic Virus (MYMV) is the main constraint in mungbean production causing substantial yield reduction up to 85 per cent. In present study, 96 F2 genotypes from the cross between GG-ABL-449 x GG-ABL-265 were subjected to natural screening to assess their resistance or susceptibility against MYMV. F2 mapping population segregated in 70 susceptible: 26 resistant genotypes i.e., 3:1 ratio showing that resistance to MYMV is governed by monogenic recessive gene. Among 96 genotypes, seven were resistant, nineteen were moderately resistant, forty-one were moderately susceptible, twenty-two were susceptible and seven were highly susceptible. All the 96 genotypes were phenotypically evaluated for seven quantitative traits under field conditions. The GCV and PCV were found higher for important yield contributing traits and seed yield is positively correlated with major yield contributing traits. The parental polymorphism study showed two markers, CYR1 and VMYR1 polymorphic out of four Resistance Gene Analogue (RGA) primers linked to MYMV resistance which showed segregation in Bulk Segregant Analysis (BSA). The markers CYR1 and VMYR1 showed differences between bulks and parents with a fragment size of 445 bp and 1236 bp respectively in resistant bulks indicating that these markers are tightly linked to the MYMV resistance gene.
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    ThesisItemOpen Access
    MOLECULAR PROFILING OF ABIOTIC STRESS RESISTANCE LOCI IN RICE (Oryza sativa L.)
    (University of Agricultural Sciences GKVK, Bangalore, 40761) ASHOK, VIMAN; HARINIKUMAR, K M
    Rice (Oryza sativa L.) is an important staple food crop that feeds half the world’s population. Among abiotic stresses, moisture stress is the 5th major problem limiting rice production in many regions of Asia. Rice is the biggest water consumer, requiring 2 to 3 times more water per unit of grain produced. Recognizing this water scarce problem globally for rice production, the better option is aerobic rice cultivation which saves almost 50 per cent of water compared to puddled situation. Generally all varieties do not perform well in aerobic condition. In this aspect, identifying different genotypes (traditional accessions and improved varieties) through allele mining in rice, where the alleles are associated with traits that enhance resistance to lower moisture stress by using trait specific SSR markers is one of the better options and plays an important role in direct selection of genotypes. Even these identified genotypes can also be used in breeding programs to improve desired agronomical traits through selection. Field evaluation of 62 different genotypes for root morphological traits, yield and yield attributed traits under aerobic condition revealed significant differences among genotypes for all traits. Root morphological traits are directly related to drought resistance, different genotypes with more root length and root number have been identified. These identified genotypes can be selected for the development of new varieties including Recombinant inbred lines (RILs), Near isogenic lines (NILs), Hybrids etc. and still there are many number of genotypes among land races and traditional varieties which can be further studied.
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    ThesisItemOpen Access
    SIGNIFICANCE OF YNK INTERACTING PROTEINS UNDER OXIDATIVE STRESS USING Saccharomyces cerevisiae MODEL SYSTEM
    (University of Agricultural Sciences GKVK, Bangalore, 41187) NAZIA, BANU; Theertha Prasad, D
    Adverse environmental conditions caused by drought, temperature, salinity are the most challenging factors that affect plant growth and survival. Organisms exposed to such stresses tend to accumulate reactive oxygen species (ROS) like hydrogen peroxide, superoxide etc. NDPKs are multi functional proteins that play an important role in cellular signaling processes involving regulation of ROS levels. Study was conducted using yeast strains like BWG7 (wild type) and ΔYNK (NDPK mutant strain). Yeast strain ΔYNK is highly susceptible to ROS compared to BWG7 based on the serial dilution, paper disc and Evans blue assay. High level expression of GST-YNK fusion protein using pGEX4T-YNK construct was achieved in E.coli BL21 strain. GST -YNK fusion protein was purified using GST sepharose affinity column and the purity was established by SDS-PAGE. GST-YNK fusion protein was conjugated to cyanogen bromide activated sepharose column and used for isolating the YNK binding proteins. Drastic reduction in the soluble protein and thermostable protein content was observed in both BWG7 and ΔYNK cells upon H2O2 treatment. SDS-PAGE showed significant differences in the protein profile in control as well as H2O2 treated BWG7 and ΔYNK cells. Increased number of YNK interacting protein was observed in BWG7 cells treated with H2O2 compared to ΔYNK. ΔYNK cells showed only one YNK interacting protein of low molecular weight in presence of H2O2. These results put together suggest that YNK is important for cellular metabolism, interact with several proteins in vivo, possibly regulate their function by its kinase activity. Deletion of YNK results in down regulation of certain proteins which could be the part of cellular signaling cascade.