DEVELOPMENT OF MYMV RESISTANCE IN GREENGRAM [Vigna radiata (L.) Wilczek] USING BULK SEGREGANT ANALYSIS
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Date
2023-01-20
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Greengram (Vigna radiata (L). Wilczek) is an important pulse crop owing to its short growth duration, low water requirement, and its suitability for crop rotation and nutritional security being a rich source of protein. Greengram’s yield is greatly affected by numerous abiotic and biotic factors. Biotic stress produced by viruses especially Mungbean Yellow Mosaic Virus (MYMV) is the main constraint in mungbean production causing substantial yield reduction up to 85 per cent. In present study, 96 F2 genotypes from the cross between GG-ABL-449 x GG-ABL-265 were subjected to natural screening to assess their resistance or susceptibility against MYMV. F2 mapping population segregated in 70 susceptible: 26 resistant genotypes i.e., 3:1 ratio showing that resistance to MYMV is governed by monogenic recessive gene. Among 96 genotypes, seven were resistant, nineteen were moderately resistant, forty-one were moderately susceptible, twenty-two were susceptible and seven were highly susceptible. All the 96 genotypes were phenotypically evaluated for seven quantitative traits under field conditions. The GCV and PCV were found higher for important yield contributing traits and seed yield is positively correlated with major yield contributing traits. The parental polymorphism study showed two markers, CYR1 and VMYR1 polymorphic out of four Resistance Gene Analogue (RGA) primers linked to MYMV resistance which showed segregation in Bulk Segregant Analysis (BSA). The markers CYR1 and VMYR1 showed differences between bulks and parents with a fragment size of 445 bp and 1236 bp respectively in resistant bulks indicating
that these markers are tightly linked to the MYMV resistance gene.
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