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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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2010 - 20194
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Subject: Agricultural Biotechnology × End date: 2019 × Start date: 2010 ×
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Now showing 1 - 4 of 4
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    ThesisItemOpen Access
    EVALUATION OF NEW INBRED LINES OF MAIZE (Zea mays L.) FOR TOLERANCE TO HEAT AND MOISTURE STRESS AND IDENTIFICATION OF POLYMORPHIC MARKERS BETWEEN CONTRASTING INBRED LINES
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE, 2019-08-03) BUKKE KUTTI BAI; Ravikumar, R. L.
    Twenty one new inbred lines of maize derived through different cycles of pollen selection for heat stress tolerance were evaluated along with nine other inbred lines for seed yield and yield per se traits under field conditions during kharif 2018. Significant differences among inbred lines were observed among inbred lines for 14 quantitative traits except number of cobs per plant, cob diameter and number of kernels per row. High PCV and GCV were observed for plant height at 45 DAS, Anthesis Silking interval (ASI), number of kernels per row and seed yield per plant. High heritability coupled with high GAM was observed for plant height at harvest and seed yield per plant. Significant variability was observed among inbred lines for seedling heat and moisture stress tolerance in the laboratory testing. The inbred lines TPGB09 and TPGB03 were identified as heat stress tolerant while the inbred lines CPGB32 and TPGB12 were considered as moisture stress tolerant at seedling stage. Inbred lines developed through three cycles of pollen selection were more tolerant to heat stress at seedling stage compared to those developed through two and one cycle of pollen selection. Seven polymorphic SSRs markers each were identified between heats stress tolerant (BTM 2) and heat sensitive (CPGB30) and moisture stress tolerant (CPGB32) and moisture stress sensitive (BTM10) inbred lines. Expression of selected abiotic stress related genes viz., Hva22, Hsp, Sus1 and Sh1 was more in heat stress tolerant inbred line compared to heat stress sensitive inbred line under heat stress.
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    ThesisItemOpen Access
    GENETIC DIVERSITY ESTIMATION FOR POD AND SEED QUALITY TRAITS IN BUSH TYPE FRENCH BEAN (Phaseolus vulgaris L.) ACCESSIONS
    (University of Agricultural Sciences, Bengaluru, 2019-09-03) ASHWINI, D.; Shyamalamma, S
    French bean is an important grain legume grown for its green pods and dry seeds. The present study on diversity estimation for pod and seed quality characters was carried out on thirty French bean accessions along with check varieties based on morphological characters and SSR markers. The accessions IC329154, EC500474, EC559576 and EC283179 showed higher number of fresh pods per plant and total fresh pod weight. The accessions EC500474, EC530843, EC512801 and IC311676 showed higher number of dry pods per plant, seeds per pod and seed length. Majority of the accessions showed slightly curved pods with pear shaped pod cross section and all the accessions possessed string. In majority of the accessions seed shape observed was truncate fastigiated and exhibited varying seed coat pattern. The dominant pod colour was yellow green and dominant seed coat colour was orange white. The SSR markers, BM175 and BM164 exhibited higher polymorphism among the accessions and varieties. The PIC value ranged from 0.19-0.36. Dendrogram constructed to assess the genetic diversity among French bean accessions and varieties using SSR marker data grouped accessions into two major clusters. The accessions mentioned above can be used in the breeding programme, for pod and seed quality traits.
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    ThesisItemOpen Access
    DEVELOPMENT OF INTRASPECIFIC LINKAGE MAP OF CHICKPEA (Cicer arietinum L.) USING SSR MARKERS TO IDENTIFY QTL’S INFLUENCING FUSARIUM WILT RESISTANCE, SEED YIELD AND YIELD RELATED TRAITS
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2016-12-23) DALPAT, LAL; Ravikumar, R. L.
    Fusarium wilt (FW) caused by Fusarium oxysporum f. sp. ciceri, is one of the important soil borne disease of chickpea. Eight races of the pathogen have been reported and race 1A is a more prevalent in India causing significant yield losses. The present study aim to validate reported linked markers for wilt resistance to race 1A, using recombinant inbred lines (RILs) derived from cross JG 62 (FW susceptible) and WR 315 (FW resistant). Forty two markers linked to FW resistance in chickpea were selected for validation. Twenty three markers were polymorphic in parents and used for genotyping the RILs. The RILs were phenotyped for wilt reaction over two seasons in sick field at ICRISAT and in sick pots in the green house condition. The linkage map with 23 markers had three linkage groups with an average marker density of 9.53 cM. Five QTLs for early wilting and three QTLs for late wilting were identified. In addition sixty polymorphic markers were used for genotyping the RILs and developed a linkage map. The map had two linkage groups with coverage of 768.92 cM and marker density of 13.73 cM. Totally 12 QTLs, five QTLs for wilt score on 30th day and seven for wilt score on 60th day were obtained for wilt reaction with a LOD score ranging from 2.75 to 9.36. The 13 markers which showed linkage to wilt resistance, were validated across nine diverse chickpea genotypes. Only two markers CS27 and CaM1402 were validated across genotypes and reliable. The marker H4G11, CaM1402, CS27 and H3A12 were found to be useful in molecular breeding for wilt resistant. Two major QTLs for days to 50% flowering, two for test weight and one for number of pods per plant were also identified by phenotyping the RILs over two seasons.
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    ThesisItemOpen Access
    EVALUATION OF T3 GENERATION TRANSGENIC TOBACCO WITH HEPATITIS B GENE FOR GROWTH, YIELD AND MOLECULAR CHARACTERS
    (University of Agricultural Sciences GKVK, Bangalore, 2010-07-21) SANGH, CHANDRAMOHAN.; RAMANJINI GOWDA, P. H.
    Hepatitis B virus (HBV) is a major cause of acute and chronic hepatitis. The current HBV vaccine is a biotechnology product that falls in the category of subunit vaccines and is made from yeast cells grown by fermentation. In recent years, a novel production system of vaccines-edible vaccines has been developed. Edible vaccines can serve multiple immunization priorities and offer advantages, including simplicity of use, lesser expense, enhanced immune responses at mucosal sites, and stimulation of humoral immunity. The plant-based production of vaccines provides new opportunities to develop oral vaccines for hepatitis B. Different host systems were employed to produce hepatitis B. In addition, a plant based HBsAg expression system makes possible the testing of an oral immunization strategy by simply feeding the plant samples. The present investigation lays emphasis on study of stability of the recombinant protein expressed in tobacco plants. Restriction digestion analysis of the gene construct pHB118 with restriction enzymes EcoRI and BamHI yielded two separate bands of 9.7kb and 3.6kb size. The presences of HBsAg gene in T3 transgenic plants were confirmed by PCR analysis. The transgenic tobacco plants showed the presence of 900 bp band in the PCR analysis. The crude protein obtained from the transformed tobacco plants were tested by SDS-PAGE for the presence of 24 kDa protein, and ELISA confirmed the antigen specificity and immunogenic nature of the Hepatitis B surface antigen. The T3 generation seeds obtained from the transgenic tobacco plants were tested for the germination in presence of kanamycin. It was observed that the segregation ratio was 3:1 indicating Mendelian inheritance. The growth parameters of T3 generation transgenic and control tobacco plants showed slight variations in growth between transgenic and control plants.