EVALUATION OF NEW INBRED LINES OF MAIZE (Zea mays L.) FOR TOLERANCE TO HEAT AND MOISTURE STRESS AND IDENTIFICATION OF POLYMORPHIC MARKERS BETWEEN CONTRASTING INBRED LINES

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Date
2019-08-03
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE
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Twenty one new inbred lines of maize derived through different cycles of pollen selection for heat stress tolerance were evaluated along with nine other inbred lines for seed yield and yield per se traits under field conditions during kharif 2018. Significant differences among inbred lines were observed among inbred lines for 14 quantitative traits except number of cobs per plant, cob diameter and number of kernels per row. High PCV and GCV were observed for plant height at 45 DAS, Anthesis Silking interval (ASI), number of kernels per row and seed yield per plant. High heritability coupled with high GAM was observed for plant height at harvest and seed yield per plant. Significant variability was observed among inbred lines for seedling heat and moisture stress tolerance in the laboratory testing. The inbred lines TPGB09 and TPGB03 were identified as heat stress tolerant while the inbred lines CPGB32 and TPGB12 were considered as moisture stress tolerant at seedling stage. Inbred lines developed through three cycles of pollen selection were more tolerant to heat stress at seedling stage compared to those developed through two and one cycle of pollen selection. Seven polymorphic SSRs markers each were identified between heats stress tolerant (BTM 2) and heat sensitive (CPGB30) and moisture stress tolerant (CPGB32) and moisture stress sensitive (BTM10) inbred lines. Expression of selected abiotic stress related genes viz., Hva22, Hsp, Sus1 and Sh1 was more in heat stress tolerant inbred line compared to heat stress sensitive inbred line under heat stress.
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