DEVELOPMENT OF INTRASPECIFIC LINKAGE MAP OF CHICKPEA (Cicer arietinum L.) USING SSR MARKERS TO IDENTIFY QTL’S INFLUENCING FUSARIUM WILT RESISTANCE, SEED YIELD AND YIELD RELATED TRAITS
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Date
2016-12-23
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Fusarium wilt (FW) caused by Fusarium oxysporum f. sp. ciceri, is one of the important
soil borne disease of chickpea. Eight races of the pathogen have been reported and race 1A
is a more prevalent in India causing significant yield losses. The present study aim to
validate reported linked markers for wilt resistance to race 1A, using recombinant inbred
lines (RILs) derived from cross JG 62 (FW susceptible) and WR 315 (FW resistant). Forty
two markers linked to FW resistance in chickpea were selected for validation. Twenty three
markers were polymorphic in parents and used for genotyping the RILs. The RILs were
phenotyped for wilt reaction over two seasons in sick field at ICRISAT and in sick pots in
the green house condition. The linkage map with 23 markers had three linkage groups with
an average marker density of 9.53 cM. Five QTLs for early wilting and three QTLs for late
wilting were identified. In addition sixty polymorphic markers were used for genotyping
the RILs and developed a linkage map. The map had two linkage groups with coverage of
768.92 cM and marker density of 13.73 cM. Totally 12 QTLs, five QTLs for wilt score on
30th day and seven for wilt score on 60th day were obtained for wilt reaction with a LOD
score ranging from 2.75 to 9.36. The 13 markers which showed linkage to wilt resistance,
were validated across nine diverse chickpea genotypes. Only two markers CS27 and
CaM1402 were validated across genotypes and reliable. The marker H4G11, CaM1402,
CS27 and H3A12 were found to be useful in molecular breeding for wilt resistant. Two
major QTLs for days to 50% flowering, two for test weight and one for number of pods
per plant were also identified by phenotyping the RILs over two seasons.
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