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University of Agricultural Sciences, Bengaluru

University of Agricultural Sciences Bangalore, a premier institution of agricultural education and research in the country, began as a small agricultural research farm in 1899 on 30 acres of land donated by Her Excellency Maharani Kempa Nanjammanni Vani Vilasa Sannidhiyavaru, the Regent of Mysore and appointed Dr. Lehmann, German Scientist to initiate research on soil crop response with a Laboratory in the Directorate of Agriculture. Later under the initiative of the Dewan of Mysore Sir M. Vishweshwaraiah, the Mysore Agriculture Residential School was established in 1913 at Hebbal which offered Licentiate in Agriculture and later offered a diploma programme in agriculture during 1920. The School was upgraded to Agriculture Collegein 1946 which offered four year degree programs in Agriculture. The Government of Mysore headed by Sri. S. Nijalingappa, the then Chief Minister, established the University of Agricultural Sciences on the pattern of Land Grant College system of USA and the University of Agricultural Sciences Act No. 22 was passed in Legislative Assembly in 1963. Dr. Zakir Hussain, the Vice President of India inaugurated the University on 21st August 1964.

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Subject: Agricultural Biotechnology × End date: 2017 ×
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Now showing 1 - 8 of 8
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    ThesisItemOpen Access
    HOST RESISTANCE, LOSS ASSESSMENT AND MANAGEMENT OF Exserohitum turcicum (pass) Leonard & Suggs, LEAF BLIGHT OF MAIZE
    (UNIVERSITY OF MYSORE, 1991) PANDURANGE GOWDA, K T
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    GENETICDIVERGENCE IN COWPEA [VIGNA UNGUICULATA (L,) WALP]
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE, 1983) CHIKKADYAVAIAH; SHIVASHANKAR, G
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    HISTOCHEMICAL AND GENETIC ANALYSIS OF NATURE OF CYTOSTERILE DIFFERNTIATION IN FOUR CYTOPLASMIC MALE STERILE LINES OF PEARL MILLET (PENNMISETUM AMERICANUM [L.] LEEKE)
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BANGALORE, 1987) CHAYA, B R; SEETHARAM, A
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    DEVELOPMENT OF INTRASPECIFIC LINKAGE MAP OF CHICKPEA (Cicer arietinum L.) USING SSR MARKERS TO IDENTIFY QTL’S INFLUENCING FUSARIUM WILT RESISTANCE, SEED YIELD AND YIELD RELATED TRAITS
    (UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU, 2016-12-23) DALPAT, LAL; Ravikumar, R. L.
    Fusarium wilt (FW) caused by Fusarium oxysporum f. sp. ciceri, is one of the important soil borne disease of chickpea. Eight races of the pathogen have been reported and race 1A is a more prevalent in India causing significant yield losses. The present study aim to validate reported linked markers for wilt resistance to race 1A, using recombinant inbred lines (RILs) derived from cross JG 62 (FW susceptible) and WR 315 (FW resistant). Forty two markers linked to FW resistance in chickpea were selected for validation. Twenty three markers were polymorphic in parents and used for genotyping the RILs. The RILs were phenotyped for wilt reaction over two seasons in sick field at ICRISAT and in sick pots in the green house condition. The linkage map with 23 markers had three linkage groups with an average marker density of 9.53 cM. Five QTLs for early wilting and three QTLs for late wilting were identified. In addition sixty polymorphic markers were used for genotyping the RILs and developed a linkage map. The map had two linkage groups with coverage of 768.92 cM and marker density of 13.73 cM. Totally 12 QTLs, five QTLs for wilt score on 30th day and seven for wilt score on 60th day were obtained for wilt reaction with a LOD score ranging from 2.75 to 9.36. The 13 markers which showed linkage to wilt resistance, were validated across nine diverse chickpea genotypes. Only two markers CS27 and CaM1402 were validated across genotypes and reliable. The marker H4G11, CaM1402, CS27 and H3A12 were found to be useful in molecular breeding for wilt resistant. Two major QTLs for days to 50% flowering, two for test weight and one for number of pods per plant were also identified by phenotyping the RILs over two seasons.
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    ThesisItemOpen Access
    EVALUATION OF T3 GENERATION TRANSGENIC TOBACCO WITH HEPATITIS B GENE FOR GROWTH, YIELD AND MOLECULAR CHARACTERS
    (University of Agricultural Sciences GKVK, Bangalore, 2010-07-21) SANGH, CHANDRAMOHAN.; RAMANJINI GOWDA, P. H.
    Hepatitis B virus (HBV) is a major cause of acute and chronic hepatitis. The current HBV vaccine is a biotechnology product that falls in the category of subunit vaccines and is made from yeast cells grown by fermentation. In recent years, a novel production system of vaccines-edible vaccines has been developed. Edible vaccines can serve multiple immunization priorities and offer advantages, including simplicity of use, lesser expense, enhanced immune responses at mucosal sites, and stimulation of humoral immunity. The plant-based production of vaccines provides new opportunities to develop oral vaccines for hepatitis B. Different host systems were employed to produce hepatitis B. In addition, a plant based HBsAg expression system makes possible the testing of an oral immunization strategy by simply feeding the plant samples. The present investigation lays emphasis on study of stability of the recombinant protein expressed in tobacco plants. Restriction digestion analysis of the gene construct pHB118 with restriction enzymes EcoRI and BamHI yielded two separate bands of 9.7kb and 3.6kb size. The presences of HBsAg gene in T3 transgenic plants were confirmed by PCR analysis. The transgenic tobacco plants showed the presence of 900 bp band in the PCR analysis. The crude protein obtained from the transformed tobacco plants were tested by SDS-PAGE for the presence of 24 kDa protein, and ELISA confirmed the antigen specificity and immunogenic nature of the Hepatitis B surface antigen. The T3 generation seeds obtained from the transgenic tobacco plants were tested for the germination in presence of kanamycin. It was observed that the segregation ratio was 3:1 indicating Mendelian inheritance. The growth parameters of T3 generation transgenic and control tobacco plants showed slight variations in growth between transgenic and control plants.
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    ThesisItemOpen Access
    MORPHOLOGICAL AND AFLP MARKER BASED GENETIC DIVERSITY IN SWEET SORGHUM WORKING GERMPLASM
    (University of Agricultural Sciences GKVK, Bangalore, 2009-07-10) RAJAPPA, P. V.; HARINIKUMAR, K. M.
    Sweet sorghum (Sorghum bicolor L.) is a type of cultivated sorghum and has been recognized widely as potential alternative source of bio-fuel because of its high fermentable sugar content in the stalk. The objectives of the study were to assess the morphological diversity and DNA marker based genetic diversity among the 30 sweet sorghum Genotypes using AFLP markers. Considerable diversity was observed for all the twelve morphological characters studied. The most important character contributing to the divergence was grain yield per plant followed by Ethanol yield; All the PCV values were higher than GCV values for each character. The highest PCV and GCV values were recorded by, cane height, number of internodes, millable stalk yield, juice yield, brix reading, ethanol yield, moderate PCV and GCV were recorded in stem girth, total sugars, reducing sugars and yield per plant and Low PCV and GCV were recorded in days to 50 % flowering. Using D2 analysis of Mahalanodis (1936), thirty genotypes were grouped into ten clusters. Among the clusters, cluster II was the largest with six genotypes followed by cluster IV and VII with five genotypes respectively. The molecular study was carried out on genetic diversity in 30 selected sweet sorghum genotypes was studied using AFLP markers. The DNA fingerprints were generated by using four EcoRI and MseI adapter specific primer combinations. The pooled binary data from four primer combinations of 30 varieties was used to develop a distance matrix to display pair-wise genetic distance between the genotypes. The dendrogram drawn for these 30 varieties gave grouping of various varieties. The major cluster in the dendrogram is sub divided into four sub clusters, the genetic distance values ranged from 2 to 37, the highest genetic distance of 37 was observed between ICSV25263 and ICSB 731. Finally, the least genetic distance of 2 was observed between ICSV111 and SSV84.
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    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF NUCLEAR POLYHEDROSIS VIRUS INFECTING Bombyx mori L. (BmNPV) AND PREVENTION OF ITS MULTIPLICATION BY USING HERBAL EXTRACTS
    (University of Agricultural Sciences GKVK, Bangalore, 2006-06-06) CHANDRAMOHAN, J.; Anitha, Peter.
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    ThesisItemOpen Access
    ISOLATION, MOLECULAR CHARACTERIZATION OF Bacillus thuringiensis AND DEVELOPMENT OF TRANSGENIC FIELD BEAN (Lablab purpureus L. Sweet) AGAINST POD BORER.
    (University of Agricultural Sciences GKVK, Bangalore, 2007-09-19) SMITHA, GABRIEL.; GOWDA, T. K. S.
    Bacillus thuringiensis (Bt) produces a variety of insecticidal crystal proteins (ICPs) upon sporulation. These proteins, called δ-endotoxins, are highly toxic to lepidopteran, dipteran and coleopteran insects. Different ICP genes of Bt have been successfully engineered into many crop-plants to obtain resistance against lepidopteran insects. The field bean (Lablab purpureus L.) grown largely in dry land conditions, is an important pulse crop that suffers from heavy pest damage. The pod borers, Helicoverpa armigera and Adisura atkisoni are two important pests that cause enormous loss. The present investigation lays emphasis on isolation, molecular characterization of Bacillus thuringiensis strains and development of transgenic field bean (Lablab purpureus L.) with Cry 1F gene against pod borer (Helicoverpa armigera). The soil samples used for isolation of Bt were collected from different parts of south India. Thirty nine Bt isolates were obtained form the fifty soil samples. All these isolates were found to be gram positive, endospore forming and crystalliferous. PCR amplification of the isolates using different primers showed that twenty four isolates were lepidopteran-specific, ten specific to coleopteran and four active against nematode. The analysis of crystal proteins of these isolates by SDS-PAGE revealed that majority of the isolates had proteins of size 120 kDa and above. Twenty four of the isolates which proved to be lepidopteran-specific were observed to be lethal to silkworm. Attempts were made to standardize an efficient regeneration protocol for field bean. Five varieties were used for the study among which selection 321 was found to perform better. Transformation of field bean was carried out using Cry 1 F gene by Agrobacterium-mediated method. Confirmation of transgene in putatively transformed plants was done by PCR analysis using Cry 1F gene specific primer. Five out of twenty five transformed plants were found to be transgenics.