MORPHOLOGICAL AND AFLP MARKER BASED GENETIC DIVERSITY IN SWEET SORGHUM WORKING GERMPLASM
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Date
2009-07-10
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University of Agricultural Sciences GKVK, Bangalore
Abstract
Sweet sorghum (Sorghum bicolor L.) is a type of cultivated sorghum
and has been recognized widely as potential alternative source of bio-fuel
because of its high fermentable sugar content in the stalk. The objectives
of the study were to assess the morphological diversity and DNA marker
based genetic diversity among the 30 sweet sorghum Genotypes using
AFLP markers. Considerable diversity was observed for all the twelve
morphological characters studied. The most important character
contributing to the divergence was grain yield per plant followed by
Ethanol yield; All the PCV values were higher than GCV values for each
character. The highest PCV and GCV values were recorded by, cane
height, number of internodes, millable stalk yield, juice yield, brix
reading, ethanol yield, moderate PCV and GCV were recorded in stem
girth, total sugars, reducing sugars and yield per plant and Low PCV and
GCV were recorded in days to 50 % flowering. Using D2 analysis of
Mahalanodis (1936), thirty genotypes were grouped into ten clusters.
Among the clusters, cluster II was the largest with six genotypes followed
by cluster IV and VII with five genotypes respectively. The molecular
study was carried out on genetic diversity in 30 selected sweet sorghum
genotypes was studied using AFLP markers. The DNA fingerprints were
generated by using four EcoRI and MseI adapter specific primer
combinations. The pooled binary data from four primer combinations of
30 varieties was used to develop a distance matrix to display pair-wise
genetic distance between the genotypes. The dendrogram drawn for
these 30 varieties gave grouping of various varieties. The major cluster
in the dendrogram is sub divided into four sub clusters, the genetic
distance values ranged from 2 to 37, the highest genetic distance of 37
was observed between ICSV25263 and ICSB 731. Finally, the least
genetic distance of 2 was observed between ICSV111 and SSV84.
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