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Chaudhary Charan Singh Haryana Agricultural University, Hisar
Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.
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ThesisItem Open Access Effect of nipping and spacing on seed yield and quality of cowpea (Vigna unguiculata)(CCSHAU, 2019) Shubham; Makhan LalThesisItem Open Access Effect of seed rate and nitrogen levels on seed yield and quality of kasuri methi (Trigonella corniculata L.)(CCSHAU, 2019) Sapra, Love; Tehlan, S.K.The experiment entitled Effect of seed rate and nitrogen levels on seed yield and quality of kasuri methi (Trigonella corniculata L.) was conducted at Research Farm of Department of Vegetable Science, CCS Haryana Agricultural University, Hisar during rabi season of 2018-19. The investigation comprising of four different seed rate and three nitrogen level was laid out in a factorial randomized block design with three replications with a plot size of 3.0 x 2.4 m. The uppermost value for plant height, pod length, number of branches per plant, number of clusters per plant, number of pods per cluster, number of pods per plant, number of seeds per pod, test weight, germination percentage, seedling length, seedling dry weight, seed vigour index I, seed vigour index II were recorded with seed rate of 7.5 kg/ha. However, seed rate of 12 kg/ha took minimum number of days to 50% flowering and to maturity. Biological yield, harvest index and seed yield were recorded highest with seed rate of 10.5 kg/ha which was at par with seed rate of 9.0kg/ha. Different nitrogen level had a significant effect on growth, seed yield and seed quality parameters. The maximum value for plant height, pod length, number of seeds per pod, biological yield, test weight, seedling length, seedling dry weight, seed vigour index I and vigour index II was recorded with nitrogen dose of 60 kg/ha. The number of branches per plant, number of cluster per plant, number of pods per cluster, number of pods per plant, seed yield, harvest index and standard germination percentage attained utmost with 40 kg/ha while days to 50 percent flowering and days to maturity recorded highest with 20 kg/ha. Interaction of various seed rates with different nitrogen levels results remarkable variation for growth, seed yield and seed quality parameters. Significantly higher values were recorded for plant height at maturity, pod length, number of seeds per pod, test weight, standard germination percentage and seedling length with treatment combination N3S1, i.e. seed rate 7.5 kg/ha and nitrogen dose of 60 kg/ha. The treatment combination N2S1, i.e. seed rate 7.5 kg/ha and nitrogen dose of 40 kg/ha, was found to be best with respect to number of branches per plant, number of cluster per plant, number of pods per cluster and number of pods per plant. The seed yield and harvest index were recorded utmost at N2S3 i.e. nitrogen dose of 40 kg/ha and seed rate of 10.5 kg/ha which was at par with N2S2 i.e. 40kg N/ha and seed rate of 9.0 kg/ha. Whereas, biological yield was utmost at N3S3 i.e. nitrogen dose of 60 kg/ha and seed rate of 10.5 kg/ha. However the days to 50 percent flowering, days to maturity, seedling dry weight, seed vigour index I and II interacted non-significantly with interaction of nitrogen and seed rate.ThesisItem Open Access Variability studies for growth, yield and quality characters of tomato (Lycopersiconesculentum Mill)(CCSHAU, 2019) Vidya R.; Batra, V.K.The present investigation entitled “Variability studies for growth, yield and quality characters of tomato (Lycopersicon esculentum Mill.)” was carried out at Research Farm of the Department of Vegetable Science, CCS Haryana Agricultural University, Hisar during a spring-summer season of 2018.The study material comprised of genetically diverse thirteen tomato genotypes which were evaluated in randomized block design and. The genotypes were taken from IIVR Varanasi in All India coordinated research project and traits were evaluated on the basis of plant height, number branches, days to 50% flowering, number of trusses per plant, number of flowers per cluster, number of fruits per truss, number of fruits per plant, leaf area index, marketable yield, average fruit weight, equatorial and polar diameter of fruit, number of locules per fruit, pericarp thickness of fruit, total soluble solids, acidity, ascorbic acid content ,chlorophyll a:b ratio, test weight of seed and days to first harvest, which differentiate the tomato genotypes. Analysis of variance studies indicated a significant difference among all the genotypes for all the characters under study. Genetic variability studies showed high PCV and GCV values for number of branches per plant (23.54 and 23.93), pericarp thickness (15.42 and 16.46) and acidity(14.79 and 15.8), indicating that a greater amount of genetic variability was present for these characters and thus, there is greater scope for further improvement by genetic manipulation. High heritability coupled with high genetic advance as per cent of mean was observed for marketable yield (98.08 %), days to 50% flowering (97.80), plant height at 90 days after transplanting (97.75%), number of fruits per plant, number of branches per plant, total soluble solids number of locules per fruit, which indicated that these traits were under the strong influence of additive gene action, and hence, simple selection based on phenotypic performance would be more effective. The total yield per plant had positive and highly significant correlation with the number of branches per plant (0.813 and 0.798), number of flowers per cluster (0.872 and 0.837), number of trusses per plant (0.861 and 0.716), number of fruits per truss , number of fruits per plant, average fruit weight, number of locules per fruit, ascorbic acid and days to first harvest at both genotypic and phenotypic levels. It indicated that the improvement in these traits leads to an increase in total yield. The highly positive direct effect on total yield was shown by The characters leaf area index, number of flowers per cluster, number of trusses per plant, number of fruits per truss, polar diameter of fruit, pericarp thickness of fruit, total soluble solids, chlorophyll a:b ratio and days to first harvest, suggested that direct selection based on these characters would result in higher breeding efficiency for improving the yield in tomato.ThesisItem Open Access Effect of integrated nutrient management on growth, yield and quality attributes in turmeric (Curcuma longa L.)(CCSHAU, 2019) Garg, Vaibhav; Bora, LilaThe present experimental study was conducted to study the effect of integrated nutrient management on growth, yield and quality attributes in turmeric (Curcuma longa L.) at Research Farm of the Department of Vegetable Science, Regional Research Station, Karnal during summer season of 2018-19 in Split-Plot Design. The findings showed that the application of 100% RDF (100:50:50 NPK kg/ha) resulted in the highest performance on germination percentage (87.78%), plant height on 60, 90, 120 and 150 DAP (16.58, 24.93, 34.41 and 34.85 cm, respectively), root dry weight (55.35 g) and shoot dry weight (15.34 g) as compared to other fertilizer levels. Among organic manures, vermicompost at 10 t/ha resulted in the highest performance on germination percentage (81.38%), plant height on 60, 90, 120 and 150 DAP (15.78, 24.61, 33.43 and 33.82 cm, respectively) and root dry weight (49.09 g). For yield attributes, integrated application of vermicompost at 10 t/ha with 100% RDF (100:50:50 NPK kg/ha) was observed best and showed the highest value for number and weight of primary and secondary rhizomes per plant closely followed by the treatment FYM at 20 t/ha with 100% RDF (100:50:50 NPK kg/ha). Yield per hectare (177.92 q) was maximum in the treatment FYM at 20 t/ha with 100% RDF (100:50:50 NPK kg/ha) closely followed by the treatment FYM at 20 t/ha with 75% RDF at 75:37.5:37.5 NPK kg/ha (171.56 q) and vermicompost at 10 t/ha in combination with 100% RDF at 100:50:50 NPK kg/ha (169.10 q).The highest values for quality attributes, viz.,dry weight of rhizomes, moisture content and curing percentage were registered with the fertilizer dose of 100% RDF at 100:50:50 NPK kg/ha closely followed by 75% RDF at 75:37.5:37.5 NPK kg/ha. Significantly better rhizomes quality in turmeric crop was observed for the application of FYM at 20 t/ha. The highest net income (Rs. 1,71,436/ha) was achieved from the treatment FYM at 20 t/ha with 100% RDF at 100:50:50 NPK kg/ha, while the maximum benefit to cost ratio (1.78) was recorded with vermicompost at 5 t/ha with 100% RDF at 100:50:50 NPK kg/ha.ThesisItem Open Access In vitro propagation and genetic fidelity testing in potato (Solanum tuberosum L.)(CCSHAU, 2019) Tejinder Singh; Panghal, V.P.S.The present experimental study was conducted to standardize an efficient protocol for rapid multiplication of two important cultivars (Kufri Pushkar and Kufri Ganga) and to check genetic fidelity using molecular markers. The findings showed that the surface sterilization with 0.2% Bavistin + 0.4% streptocyclin for 45 minutesfollowed by 0.1% HgCl2 treatment for 60 seconds was optimum for in vitro culture establishment and maximum survival percentage. In cv. Kufri Pushkar,the minimum number of days (3.3) required for bud initiation and highest number of buds/explant (3.6) were obtained on MS medium fortified with 0.25 mg/l BAP + 0.5 mg/l KIN, while, in cv. Kufri Ganga, 3.2 days were required for bud initiation and 3.4 buds were obtained on the same medium. Maximum number of shoots/explant (4.4) were recorded when auxins were used in combination with cytokinins at a concentration of 0.01 mg/l IAA + 0.25 mg/l BAP in cv. Kufri Pushkarand 4.7 shoots per explant were observed in cv. Kufri Ganga. Number of days required for root initiation (2.3) was recorded to be minimum on rooting medium consisting of MS medium+2.5 mg/l NAA and MS medium + 1.5 mg/l NAA in cv. KufriPushkarand 2.2 days in cv. Kufri Ganga. Maximum number of roots/explant (14.0) were observed on MS medium fortified with 2.0 mg/l IBA in cv. Kufri Pushkarwhile 14.2 number of roots were recorded in cv. Kufri Ganga on 21th day of subculturing. Maximum 100 % rooting was observed in all MS media supplemented with different concentrations of auxins. The highest survival per cent (100%) of in vitro raised plants was recorded in potting media containing cocopeat, perlite and vermiculite in 3:1:1 ratio in both the cultivars. In vitro raised plants were assessed for genetic fidelity by using twenty RAPD primers. Out of twenty primers screened, four primers produced amplification while sixteen primers did not show any results in both the cultivars Kufri Pushkar and Kufri Ganga.DNA banding pattern of all tissue culture raised plants and mother plant was found similar and monomorphic explaining that all the plants raised through tissue culture using shoot tips were true to type and genetically identical to the mother plant.ThesisItem Open Access Variability studies in bitter gourd (Momordica charantia L.)(CCSHAU, 2019) Mehta, Tanvi; Duhan, D.S.The present investigation entitled “Variability studies in bitter gourd (Momordica charantia L.)” was carried out at Research Farm of the Department of Vegetable Science, CCS Haryana Agricultural University, Hisar during a spring-summer season of 2018.The study material comprised of genetically diverse 27 bitter gourd genotypes which were evaluated in randomized block design with three replications. The genotypes were taken from IIVR Varanasi and Department of Vegetable Science, CCSHAU, Hisar. These genotypes were evaluated on the basis of morphological traits like leaf shape, leaf pubescence, fruit neck, fruit skin colour, fruit shape in longitudinal section, shape of fruit at blossom end, shape of fruit at peduncle end and also on the basis of 16 quantitative characters viz., days to 50% germination, number of primary branches, days to first male flower opening, days to first female flower opening, nodes to first male flower, nodes to first female flower, leaf length (cm), leaf width (cm), leaf blade, days to first fruit harvest, length of fruit (cm), diameter of fruit (cm), vine length at the time of final harvest (m), weight of 100 seeds (g), number of fruits per vine and fruit yield per vine (kg). Analysis of variance studies exhibited highly significant variation among all the genotypes for all the characters under study except nodes to first male flower, nodes to first female flower, leaf length (cm). High genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) values were recorded for the characters number of fruits per vine (26.41 and 26.64) and number of primary branches (22.50 and 23.14) suggesting a wider range of genetic variability present for these characters. High heritability along with high genetic advance was found in case of number of fruits per vine (95.18 and 53.96), number of primary branches (85.20 and 45.06), weight of 100 seeds (98.98 and 37.26), fruit yield per vine (96.64 and 31.03) showing strong impact of additive gene effect, encouraging direct selection of these traits for further use in yield improvement of bitter gourd. The fruit yield per vine in kg had positive and highly significant correlation with number of primary branches (0.662 and 0.627), leaf length (0.291 and 0.252), length of fruit (0.518 and 0.411) and diameter of fruit (0.495 and 0.389) at both genotypic and phenotypic levels. It inferred that the improvement in these traits will directly lead to an increase in total fruit yield. Among all the 16 quantitative characters, eight characters showed positive direct effect on fruit yield per vine at genotypic level that is, number of fruits per vine (1.4136), nodes to first male flowering (0.5390), length of fruit (0.5376), days to 50% germination (0.4042), leaf blade (0.2574), days to first female flowering (0.0748), leaf length (0.0717) and days to first fruit harvest (0.0048). The genotypes viz., IC 85624 followed by HK 113, IC 85610, IC 85605 were found promising in terms of fruit yield and related traits which could be further utilized for future breeding programmes.ThesisItem Open Access Micropropagation and genetic fidelity testing in potato(CCSHAU, 2019) Rout, Bichhinna Maitri; Bhatia, A.K.The present experimental study was conducted to standardize an efficient protocol for rapid multiplication of two important cultivars (Kufri Neelkanth and Kufri Lima) and to check genetic fidelity using molecular markers. The findings showed that the surface sterilization with 0.2% Bavistin + 0.4% streptocyclin for 45 minutes followed by 0.1% HgCl2 treatment for 55 seconds was optimum for in vitro culture establishment and maximum survival percentage. In cv. Kufri Neelkanth the minimum number of days (3.3 days) required for bud initiation and highest number of buds/explant (3.5) were in MS media fortified with 0.5 mg/l BAP + 0.5 mg/l KIN while 3.2 days were required for initiation and 3.2 buds were formed in cv. Kufri Lima on the same. The maximum number of shootlets/explants (10.4) were recorded when auxins were used in combination with cytokinins at a concentration of 0.01 mg/l NAA + 0.25 mg/l KIN in cv. Kufri Neelkanth and 10.1 shootlets per explants were observed in cv. Kufri Lima. The number of days required for root initiation (2.1) were recorded to be minimum in rooting media consisting of MS medium+2.5 mg/l NAA in cv. Neelkanth and 2.2 days in cv. Kufri Lima. The maximum number of roots/explants (13.0) were observed when MS media fortified with 2.5 mg/l IBA in cv. Kufri Neelkanth while 12.6 number of roots were recorded on MS media fortified with 2.0 mg/l IBA in cv. Kufri Lima. Maximum 100 % rooting was observed in all MS media supplemented with different concentrations of auxins. The highest survival per cent (100%) of in vitro raised plants was recorded in potting media containing cocopeat, perlite and vermiculite in 3:1:1 ratio in both the cultivars. In vitro raised plants were assessed for genetic fidelity by using twenty RAPD primers. Out of twenty primers screened, two primers produced amplification while eighteen primers did not show any amplification in cv. Kufri Neelkanth whereas four primers produced amplification while sixteen primers did not show any amplification in. cv. Kufri Lima. DNA banding pattern of all tissue culture raised plants and mother plant was found similar showing monomorphism explaining that all the plants raised through tissue culture using shoot tips were true to type or genetically identical to the mother plant.