In vitro propagation and genetic fidelity testing in potato (Solanum tuberosum L.)
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Date
2019
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CCSHAU
Abstract
The present experimental study was conducted to standardize an efficient protocol for rapid multiplication of two important cultivars (Kufri Pushkar and Kufri Ganga) and to check genetic fidelity using molecular markers. The findings showed that the surface sterilization with 0.2% Bavistin + 0.4% streptocyclin for 45 minutesfollowed by 0.1% HgCl2 treatment for 60 seconds was optimum for in vitro culture establishment and maximum survival percentage. In cv. Kufri Pushkar,the minimum number of days (3.3) required for bud initiation and highest number of buds/explant (3.6) were obtained on MS medium fortified with 0.25 mg/l BAP + 0.5 mg/l KIN, while, in cv. Kufri Ganga, 3.2 days were required for bud initiation and 3.4 buds were obtained on the same medium. Maximum number of shoots/explant (4.4) were recorded when auxins were used in combination with cytokinins at a concentration of 0.01 mg/l IAA + 0.25 mg/l BAP in cv. Kufri Pushkarand 4.7 shoots per explant were observed in cv. Kufri Ganga. Number of days required for root initiation (2.3) was recorded to be minimum on rooting medium consisting of MS medium+2.5 mg/l NAA and MS medium + 1.5 mg/l NAA in cv. KufriPushkarand 2.2 days in cv. Kufri Ganga. Maximum number of roots/explant (14.0) were observed on MS medium fortified with 2.0 mg/l IBA in cv. Kufri Pushkarwhile 14.2 number of roots were recorded in cv. Kufri Ganga on 21th day of subculturing. Maximum 100 % rooting was observed in all MS media supplemented with different concentrations of auxins. The highest survival per cent (100%) of in vitro raised plants was recorded in potting media containing cocopeat, perlite and vermiculite in 3:1:1 ratio in both the cultivars. In vitro raised plants were assessed for genetic fidelity by using twenty RAPD primers. Out of twenty primers screened, four primers produced amplification while sixteen primers did not show any results in both the cultivars Kufri Pushkar and Kufri Ganga.DNA banding pattern of all tissue culture raised plants and mother plant was found similar and monomorphic explaining that all the plants raised through tissue culture using shoot tips were true to type and genetically identical to the mother plant.
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