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Chaudhary Charan Singh Haryana Agricultural University, Hisar

Chaudhary Charan Singh Haryana Agricultural University popularly known as HAU, is one of Asia's biggest agricultural universities, located at Hisar in the Indian state of Haryana. It is named after India's seventh Prime Minister, Chaudhary Charan Singh. It is a leader in agricultural research in India and contributed significantly to Green Revolution and White Revolution in India in the 1960s and 70s. It has a very large campus and has several research centres throughout the state. It won the Indian Council of Agricultural Research's Award for the Best Institute in 1997. HAU was initially a campus of Punjab Agricultural University, Ludhiana. After the formation of Haryana in 1966, it became an autonomous institution on February 2, 1970 through a Presidential Ordinance, later ratified as Haryana and Punjab Agricultural Universities Act, 1970, passed by the Lok Sabha on March 29, 1970. A. L. Fletcher, the first Vice-Chancellor of the university, was instrumental in its initial growth.

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  • ThesisItemOpen Access
    Cultivar identification in Soybean
    (CCSHAU, 2005) Ali, N.Mohammed; Verma, S.S.
    The present investigation comprised of 15 soybean cultivars. To substantiate results seed characters and biochemical test were studied in laboratory and plant morphological characters observed in the field. In seed characteristics hilum colour was most important for identification of off type plants. Among plant morphological characters hypocotyl colour, flower colour, pubescence colour and density were important qualitative traits in soybean cultivar identification and were highly stable and heritable. Growth habit, day to maturity, pod shape, seed shape, seed size, seed coat colour, stem termination type and pod colour too may be specific in expression and therefore, serve in identification of cultivars. Among biochemical characters peroxidase test was most important and not coincide with any other characters and not affected by the storability and quality of seed. The protein-banding pattern clearly differentiates the cultivars into different groups. In gradient gel more number of bands could identified than the single percentage gel. Studying the both morphological and biochemical characters either of the alone characters can’t differentiate the cultivars into clear-cut groups. Thus a combination of both morphological and biochemical characters are most important in identification of cultivars. By these characters an identification key could be developed for easy identification at any stage of seed multiplication. A minimum of three crop observations at seedling, flowering and maturity stages were critical for characterization of soybean cultivars on morphological characters.
  • ThesisItemOpen Access
    Effect of pickings on seed quality in cotton (Gossypium hirsutum and Gossypium arboreum)
    (CCSHAU, 2005) Sasan, Ashish Kumar; Duhan, J.C.
    Seed quality plays an important role in crop establishment and overall performance of the crop. Four picking stage viz. First, Second, Third, Fourth of the eight varieties/hybrids viz. AAH-1, HD-107, HD-123, HD-324, HHH-223, H-1098, H-1117, H-1226 of cotton (Gossypium hirsutum and Gossypium arboreum) were evaluated by various tests viz. Standard germination, Seedling length, Seedling dry matter, Vigour index-I, Vigour index-II, Dehydrogenase activity test, Electrical conductivity, Seed index, Seed density, Tetrazolium test in the Department of Seed Science and Technology at Chaudhary Charan Singh Haryana Agricultural University, Hisar during 2003-2004. The quality of second picking seed was found significantly better as comparision to other pickings in all varieties/hybrids except seed index which was observed superior in the first picking seed.AAH-1 in desi cotton and HHH-223 variety in American cotton were found superior in seed quality and storability as compare to other varieties. In desi and American cotton AAH-1 and HHH-223 in second picking were recorded significantly higher interaction effect as compared to other combinations (pickings/varieties). These seed lots of various pickings were accelerated aged at 42±1oC for 72 hrs. for predicting the storability. The storability of second picking seed was observed significantly better as comparison to other pickings in all varieties / hybrids. AAH-1 in desi cotton and HHH-223 variety in American cotton wee found superior in storability as compare to other variation.
  • ThesisItemOpen Access
    Effect of ageing and priming on seed storage proteins of barley. (Hordeum vulgare L.)
    (CCSHAU, 2005) Chauhan, Davender Singh; Punia, R.C.
    Barley owing to its suitability for salinity and water stress tolerance was selected to define variability available in improved cultivars for seed vigor, viability and storability. An effort was also made to study the effect of ageing and priming on seed storage proteins on the basis of total seed protein profiles. To undertake the above studies six improved genotypes viz. Alfa-93, BH-393, BH-75, BCU-73, RD-2552, K-551 were taken define the changes in storage protein in fresh and accelerated aged seeds. It was observed that all the six genotypes were quite variable for seed quality parameters. The fresh seed lots of all the genotypes were having standard germination above 90%. However, accelerated ageing reduced it to nearly 50%, owing an adverse effect on all the seed quality parameters. On the basis of seed storage protein profiles of fresh and accelerated aged seeds it was revealed that protein profile of fresh seeds was less variable over accelerated aged seeds. An effort was also made to enhance the value of deteriorated seeds (accelerated aged) of BH-75 and BH-393 by giving priming treatments. It was revealed that priming with water and aqueous solution of PEG 6000 significantly increased the standard germination, rate of germination, dehdrogenase activity and vigor index by boosting the repair mechanism of seed. The protein profile of primed seeds revealed that priming results in hydrolysis of seed storage proteins.
  • ThesisItemOpen Access
    Effect of pickings on seed quality in cotton (Gossypium hirsutum and Gossypium arboreum)
    (CCSHAU, 2005) Sasan, Ashish Kumar; Duhan, J.C.
    Seed quality plays an important role in crop establishment and overall performance of the crop. Four picking stage viz. First, Second, Third, Fourth of the eight varieties/hybrids viz. AAH-1, HD-107, HD-123, HD-324, HHH-223, H-1098, H-1117, H-1226 of cotton (Gossypium hirsutum and Gossypium arboreum) were evaluated by various tests viz. Standard germination, Seedling length, Seedling dry matter, Vigour index-I, Vigour index-II, Dehydrogenase activity test, Electrical conductivity, Seed index, Seed density, Tetrazolium test in the Department of Seed Science and Technology at Chaudhary Charan Singh Haryana Agricultural University, Hisar during 2003-2004. The quality of second picking seed was found significantly better as comparision to other pickings in all varieties/hybrids except seed index which was observed superior in the first picking seed.AAH-1 in desi cotton and HHH-223 variety in American cotton were found superior in seed quality and storability as compare to other varieties. In desi and American cotton AAH-1 and HHH-223 in second picking were recorded significantly higher interaction effect as compared to other combinations (pickings/varieties). These seed lots of various pickings were accelerated aged at 42±1oC for 72 hrs. for predicting the storability. The storability of second picking seed was observed significantly better as comparison to other pickings in all varieties / hybrids. AAH-1 in desi cotton and HHH-223 variety in American cotton wee found superior in storability as compare to other variation.
  • ThesisItemOpen Access
    Characterization and vigour assessment of cluster bean (Cyamopsis tetragonoloba (L.) Taub.)
    (CCSHAU, 2006) Rana, Rakesh; Deswal, D.P.
    A study was conducted on “Characterization and vigour assessment of cluster bean (Cyamopsis tetragonoloba (L.) Taub.)” by applying some field and laboratory tests. Plant morphological characters like plant growth habit, leaf pubescence, leaf margin, flower colour and pod shape were found to be most important diagnostic characters for cultivar identification in cluster bean. Some other character such as number of pod per cluster, days to 50 per cent flowering and pod size were also helpful in identification but cannot be referred as reliable trait for cultivar identification because of effect of location and management. Some seed morphological characters like 100-seed weight and seed shape were also found helpful to differentiate the cluster bean cultivars. The differential response of cluster bean cultivars to different chemical tests such as phenol test, peroxidase test, KOH-bleach test and potassium hydroxide test was found to be accurate means of classification which shows the reliability and quickness of these chemical tests. The schematic diagram on the basis of morphological traits has higher resolution in terms of differentiating among the genotypes. On the other hand the laboratory tests though not so laborious but has less resolving capacity to differentiate among genotypes. The significant mean sums of squares due to genotypes for all the seed vigour parameters revealed the existence of sufficient amount of variability. The correlation study showed the significant and positive association among all the seed quality parameters except electrical conductivity, which showed negative association with other quality parameters.
  • ThesisItemOpen Access
    Effect of priming on seed quality in pigeonpea. (Cajanus cajan L. millsp.)
    (CCSHAU, 2006) Ashok Kumar; Kashyap, R.K.
    Experiment was conducted to study the effect of priming on seed quality in pigeonpea and also to study the various enzyme activities. To undertake this study two improved variety viz. Manak and Paras were studied. Each variety was grouped into two seed lots on the basis of germination i.e. L1 good seed lot L2 marginal seed lot. It was observed that marginal seed lot L2 performed better after applying pre-sowing treatments under laboratory and field condition. And among varieties, Paras performed better in field condition than Manak in all the priming treatments. Among all the treatment only GA3 (50ppm), cold hydration and cold dehydration +0.25% thiram showed positive results in both the varieties and lots. Treatment cold dehydration +0.25% thiram showed highest improvement in germination, vigour index-I, plumule length and dry weight in both the seed lots. An effort was also made to enhance the germination of marginal seed lot by applying CaCl2 and KNO3 priming treatments but the experiment showed negative results in both the treatments in pigeonpea. It was revealed that variety Manak was showed good enzyme activities than the Paras. And among seed lots lot L1 showed good enzyme activities than lot L2.
  • ThesisItemOpen Access
    Effect of seed priming on viability and vigour of cowpea (Vigna unguiculata L. Walp) seed
    (CCSHAU, 2007) Maniktahla, Saurabh; Verma, S.S.
    In the present investigation, two seed lots of four varieties viz., HC2-85, HC3-8, HC98-64 and FS-68 were taken and grouped into two seed lots on the basis of germination percentage. The lot which showed germination percentage above the Indian Minimum Seed Certification Standard (75.0 per cent) of cowpea were termed as good seed lot (L1) and which showed germination percentage below the Indian Minimum Seed Certification Standard of cowpea were termed as marginal seed lot (L2). All the eight seed lots were given the five pre-sowing seed treatments viz., hydration-dehydration (for 4 h), GA3 (50ppm), GA3 (100ppm), KNO3 (0.1%) and hydration-dehydration + thiram @ 3g/kg seed treatments and compared with control. The results revealed that marginal seed lot (L2) showed more improvement than good seed lot (L1) after pre-sowing seed treatments under laboratory and field conditions. Among the varieties, HC3-8 showed maximum improvement in standard germination while HC2-85 showed maximum improvement in seedling establishment. The results also predicted that the treatment GA3 (100 ppm) followed by hydration dehydration + thiram @ 3g/kg seed was found most effective in all the parameters except in root length in which hydration dehydration + thiram @ 3g/kg seed was found most effective among all the treatments.
  • ThesisItemOpen Access
    Studies on seed storability in groundnut (Arachis hypogaea L.)
    (CCSHAU, 2007) Jha, Kailash Kumar; Kharb, R.P.S.
    Seeds of two groundnut cultivars MH-4 and MH-34 were dried to about 5% moisture content and stored with or without shells under ambient as well as controlled conditions in gunny bags and polythene bags to determine their effect on storage potential of seed samples. Germination was recorded quarterly and it did not differ among seed samples stored for upto 6 months, but afterwards both germination and vigour index exhibited significant reduction, irrespective of the storage environment and packaging material. At the end of storage period (12 months), the reduction in the values of germination ranged from 19.5-39.6 per cent among different combinations of storage factors. Samples stored in polythene bags and controlled conditions maintained the minimum seed certification standards even after 12 months of storage period and this combination found superior to others for storage of groundnut produce. In order to standardize the accelerated ageing technique, it was observed that as the temperature and ageing period advanced, the germination percentage declined in both kernels and pods. Maximum reduction in germination was observed after 96 hours in kernels and 168 hours in pods at 41ºC. The germination percentages were below the minimum seeds standards for this crop. Thus these conditions were found optimum for performing accelerated ageing test to know the relative storability of seed lots of groundnut varieties. An effort was also made to assess the effect of seed priming treatments on field emergence. Present study revealed that seed treated with GA3 (50 ppm) gave comparatively better results among various treatments for the characters total field emergence and field emergence index in both the varieties while soaking in water in case of MH-4 and hydration dehydration in MH-34 reduced the overall mean germination time.
  • ThesisItemOpen Access
    Characterization of hybrids and their parental lines in cotton(Gossypium spp.)
    (CCSHAU, 2007) Mohana Kumara. M.R.; Punia, R.C.
    In the present investigation, seven cotton hybrids and their parental lines were studied at CCS Haryana Agricultural University, Hisar during 2006-2007.All the genotypes were studied for characterization based on morphological characters and laboratory techniques. The morphological characters such as Hypocotyl pigmentation, plant height, growth habit, stem hairiness, leaf shape, leaf appearance, leaf pubescence, leaf lobe number, boll surface, boll shape, boll colour,gossypol glands, petal colour, bract type, bract number of serration, anther colour, petal spotting, position of the stigma and petiole pigmentation were found as the most important parameters for characterization. The genotypes were also subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of total soluble seed proteins. The findings showed that SDS-PAGE of seed proteins resolve total of 23 bands which were present in different position with different intensity. Band number 19 was present only in Gossypium hirsutum genotypes differentiated from the Gossypium arboreum genotypes. The Flow charts were prepared based on morphological characters and protein fingerprints. The identification keys were also prepared on the stable morphological characters. All the genotypes were differentiated using morphological characters and protein molecular markers.