Characterization of hybrids and their parental lines in cotton(Gossypium spp.)
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Date
2007
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Publisher
CCSHAU
Abstract
In the present investigation, seven cotton hybrids and their parental
lines were studied at CCS Haryana Agricultural University, Hisar during
2006-2007.All the genotypes were studied for characterization based on
morphological characters and laboratory techniques. The morphological
characters such as Hypocotyl pigmentation, plant height, growth habit,
stem hairiness, leaf shape, leaf appearance, leaf pubescence, leaf lobe
number, boll surface, boll shape, boll colour,gossypol glands, petal colour,
bract type, bract number of serration, anther colour, petal spotting,
position of the stigma and petiole pigmentation were found as the most
important parameters for characterization. The genotypes were also
subjected to sodium dodecyl sulphate polyacrylamide gel
electrophoresis (SDS-PAGE) of total soluble seed proteins. The
findings showed that SDS-PAGE of seed proteins resolve total of 23
bands which were present in different position with different
intensity. Band number 19 was present only in Gossypium hirsutum
genotypes differentiated from the Gossypium arboreum genotypes.
The Flow charts were prepared based on morphological characters
and protein fingerprints. The identification keys were also prepared
on the stable morphological characters. All the genotypes were
differentiated using morphological characters and protein molecular
markers.
Description
Keywords
Genotypes, Sowing, Cotton, Electrophoresis, Proteins, Hybrids, Planting, Diseases, Developmental stages, Vegetables