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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Prevalence and characterization of non-typhoidal Salmonella isolates obtained from retail fish meat shops
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-02) Sana Parveen; Sana Parveen; Sana Parveen; Maansi; Maansi; Maansi
    Non-Typhoidal salmonellosis stands among the major food illnesses. Outbreaks from contaminated fishes have been witnessed worldwide. This study was performed to assess the presence of Non -Typhoidal Salmonella in fishes and associated environmental samples of 22 retail fish meat shops of 4 locations of Uttarakhand. For this, a total of 368 samples (fish meat swabs(n=46), gill swabs(n=23), skin swabs(n=25), intestine swabs(n=23), hand swabs(n=44), knife swabs(n=27), rinsing water(n=22), fish water(n=41), floor swabs(n=22), chopping board swabs(n=24), utensil swabs(n=24) and container swabs(47) were collected. The overall occurrence of Non-Typhoidal Salmonella was found to be 4.35% (16/368). The highest prevalence of Salmonella was observed in rinsing water (18.18%,4/22) sample followed by knife swabs ( 11.11%, 3/27) , chopping board swabs (8.33%, 2/24) , meat swabs (6.52%, 3/46) , floor swab (4.54%,1/22) , gill and intestine swab (4.34%, 1/23), fishwater (2.43%,1/41). Geographically, the highest prevalence was observed in Lalkuan (6.52%, 3/46) followed by Haldwani (5.55%, 10/180), Kiccha (2.12%, 1/47) and Pantnagar (2.08%, 2/96). Of 16 Salmonella isolates, confirmed using ompC (204 bp) gene, 14 were revealed as S.Typhimurium (87.5%, 14/16) while 2 did not exhibit the typh gene (401bp) amplicon size. All the 16 isolates screened for the presence of virulence genes using PCR commonly harbored sipA gene (87.5%, 14/16) followed by stn (75%, 12/16), sopB ( 68.75%, 11/16), sopE1 ( 56.25%, 9/16) mgtC ( 43.75%, 7/16) and spvC and gipA ( 12.5%, 2/16) genes each. Highest resistance was observed against Tetracycline and Ampicillin (93.75%, 15/16), Nalidixic acid (50%, 8/16), Ciprofloxacin (37.5%, 6/16) Ofloxacin, Cefotaxime and Sulfisoxazole ( 25%, 4/16) each, Chloramphenicol (12.5%, 2/16) and Streptomycin ( 6.25%, 1/16). Ten Salmonella isolates were multi drug resistant (MDR). Ten different antimicrobial resistance patterns were observed. Of these, only one pattern (TE, AMP, NA, CTX , OF, SF) was found common in 2 Salmonella isolates belonging to knife and meat swab sample. All phenotypically resistant and intermediate resistant isolates were screened for 6 corresponding antimicrobial resistance genes. The most commonly occurring resistance gene was gyrA (92.30%, 12 /13), blaTEM (53.33%, 8/15), aadA1 and strA (50%, 2/4), sul1 (30.76 %, 4/13) while tetA was not found in any of the isolates. Overall, our study detected occurrence of Non-Typhoidal Salmonella in the fish retail meat shops. Resistance to critically important flouroquinolones and highly important Cephalosporin and Tetracycline antibiotic detected in Salmonella isolates is a serious threat to public health which highlights the indiscriminate use of antimicrobials.
  • ThesisItemOpen Access
    Whole genome sequencing & bioinformatics of Campylobacter isolated from animals
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2020-11) Roma; Upadhyay, A.K.
    Campylobacter species are one of the leading cause of bacterial foodborne zoonoses. Worldwide, Pathogenic Campylobacter species are responsible for causing over 400–500 million infections cases each year. These pathogenic Campylobacter species are grouped into major human enteric pathogens (C. jejuni, C. jejuni subsp. jejuni (Cjj), C. jejuni subsp. doyley (Cjd), C. coli and C. fetus) minor pathogens (C. concisus, C. upsaliensis, C. lari and C. hyointestinalis) and major veterinary pathogens (C. fetus subsp. venerealis (Cfv) and C. Fetus subsp. fetus (Cff)). A total of 498 samples consisting of poultry caeca (120), litter (40), poultry rectal swabs (24), poultry skin (24), water samples (38),faeces of pigs (30), meat swabs (32), clinical dog faecal ssample (20), goat (32) and lab animals faeces (92) were screened for the presence of C. jejuni and C. coli using conventional isolation and identification procedures. The overall occurence of Campylobacter was 8.83 %. The samples collected were screened for campylobacters. Highest isolation rate was observed from lab animals (19.56 %, 18/92) followed by poultry caeca (13.33%,16/120), pig faeces (10%,3/30), meat swabs (9.37 %, 3/32) ,poultry faeces (7.14%, 3/42) and litter (2.5%, 1/40). Since loads of virulence genes are responsible for bacterial pathogenecity, resilence, stress response and environmental persistence. The individual detection of every gene by techniques like PCR is a tedious process. The next generation sequencing concept play an extremely important role in this context. Among next generation sequencing also, whole genome sequencing (WGS) and assembly is best method for the characterization of isolates as it can detect every gene of the organism coding for it’s identification, virulence, pathogenicity and antibiotic resistance.The study characterised the entire genome of the infectious isolates by analysis of WGS sequence data via use of both web based and command based tools. Virulence genes pattern was observed using web based analysis tools namely VFDB Virulence Factor Database (http://www.mgc.ac.cn/VFs) and wgMLST (Whole-genome MLST) which is pangenome based tool was used using core genome genes/loci and all acessory genes/loci to detect lineage – specific genes/loci. A total of 23 genes were detected by VFDB and 33 virulence genes were detected via SRST2. Only resistance to beta – lactam were found. Overall, our study detected only one antimicrobial resistance gene that is blaₒₓₐ -₄₈₉ commonly found in Campylobacter spp. indicating good antibiotic management in areas of Pantnagar, Rudrapur and Barielly
  • ThesisItemOpen Access
    Molecular genotyping of multidrug-resistant Salmonella typhimurium isolates using repetitive sequence-based PCR fingerprinting technique
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2020-08) Sivakumar, V.; Maansi
    Non-typhoidal salmonellae, responsible for salmonellosis in humans, are a threat to food safety impacting human health in the form of hospitalizations and in severe cases, fatalities. Such an impact also results in huge economic losses. Several food borne outbreaks resulting from zoonotic non- typhoidal Salmonella serovars have been reported, of which, S. Typhimurium has shown predominance. Epidemiological investigations of an outbreak requires accurate identification of the infection source and the transmission route for effective implementation of preventive measures against microbial food-borne pathogens. For this, techniques which can detect differences among the isolates at genomic level are applied. Therefore, in the present investigation, multidrug-resistant isolates of Salmonella Typhimurium were genotyped using repetitive sequence-based PCR (rep-PCR) fingerprinting. A total of 45 MDR S. Typhimurium isolates belonging to various sources and locations were procured from the Department of Veterinary Public Health and Epidemiology, CVASc, GBPUAT, Pantnagar. All the isolates were tested for purity and were confirmed on the basis of cultural, biochemical, serotyping and molecular (duplex PCR targeting genus specific ompC gene and serovar specific typh gene) assay. The isolates were subjected to ERIC, REP, BOX and GTG5-PCR analysis after DNA extraction and PCR confirmation. ERIC, REP, BOX and GTG5-PCR fingerprinting generated reproducible band patterns ranging from 3-10, 1-5, 6-14 and 2-10 separate bands, respectively. Cluster analysis revealed 9 types from ERIC-PCR, 7 types from REP-PCR, 16 types from BOXPCR and 9 types from GTG5-PCR. Hundred percent typeability was obtained with all genotyping techniques except REP-PCR, which differentiated 39 Typhimurium isolates only (86.6%). The isolates’ sharing similar band patterns is suggestive of a genetic similarity between them directing towards the multifactor involvement in the transmission of Salmonella. Faecal isolates and meat swab isolates sharing identical band patterns and grouped into a single cluster is indicative of a likely cross contamination. No unique pattern was observed in penta resistant (ACSSuT) profile isolates by our typing techniques. DI value of BOX-PCR was highest (0.940) followed by GTG5-PCR (0.862), REP-PCR (0.846) and ERIC-PCR (0.843). On comparing the techniques, the BOX-PCR exhibited good DI value, typeability and complex band pattern on gel in differentiating the isolates. Hence, to conclude, the BOX-PCR fingerprinting technique was found useful in the genotyping of isolates suitable enough to find its application in an epidemiological investigation during an outbreak.
  • ThesisItemOpen Access
    Whole genome sequencing of nontyphoidal Salmonella isolates recovered from humans, swine and environment
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2020-01) Pathak, Anubha Prashant; Upadhyay, A.K.
    Nontyphoidal Salmonella (NTS) is a serious zoonotic concern worldwide. It is one of the most important causes of diarrhea due to the consumption of food of animal origin such as pork, chicken and eggs. Armed with a battery of virulence factors and equipped with chromosomal and plasmid mediated antimicrobial resistance (AMR), Salmonella has established itself as successful zoonotic pathogen. Whole Genome Sequencing (WGS) is increasingly replacing various molecular typing and subtyping techniques used in bacteria as it offers highest resolution as well as detailed information on the accessory genes. Keeping these assertions in perspective, this work was designed to study the serotypes, AMR genes, virulence genes and plasmids in the Salmonella isolates (n=90) obtained from swine, human and swine rearing environment using WGS. The WGS of all the isolates (n=90) was conducted using Illumnia Miseq platform. The analysis of sequence data using various online and command-line based tools revealed a stereotypically diverse population of bacteria. A total of 15 serotypes were identified. The isolates were further classified using eBGSs (eBurst Groups), MLST (Multi Locus Sequence Typing) and rST (Ribosomal Sequence typing). Isolates grouped into 16 eBGs, 18STs and 25 rSTs offering more information than serotyping alone. The wgMLST was used to construct the phylogenetic tree which revealed serotype-based clustering and rST based sub-clustering. The virulence gene analysis revealed a highly conserved repertoire of genes. A total of 115 genes were detected, all the isolates (100%) carried genes encoding for type three secretion system one (T3SS1) and two (T3SS2) and their effectors, nonfimbrial adherence determinants, macrophage inducible genes and genes aiding in Mg2+transport. However, six genes constituting the T3SS2 effectors gogB, sseK1, sseK2, sseI, sspH1, sspH2 showed a serotype specific distribution pattern. A total of 27.7% of the isolates, all consisting of the serotype Typhimurium, from the three sources, were found to carry IncF plasmid mediated highly virulent genes spv, rck and pef. Typhoidal toxin gene cdtB was reported in 11.1% of NTS isolates. A total of 30 antimicrobial resistance genes encoding for resistance to 9 groups of antimicrobials were detected. The gene sul1 (45.5%) was most commonly detected, followed by tetA (30%) and ant(3'')-Ia (28%). Notably, two recently discovered genes encoding for resistance to the most crucial antibiotics Fosfomycin (fosA7) and Colistin (mcr9) were also reported. Out of the 19 plasmids identified, The pSPCV (IncFII) plasmid was found in 30% of the isolates followed by pSLT-BT (IncFIB) in 27.7% of isolates. A total of 8 groups of coexisting genes were found, ant(3'')-Ia blaCARB-2 floR_2 sul1 tet(G) which is responsible for the penta- resistance (ACSSuT) pattern of Salmonella Typhimurium was the most commonly reported group.
  • ThesisItemOpen Access
    Detection and quantification of chlorpyrifos and endosulphan residues in dead animal’s using high performance liquid chromatography
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2007-05) Tyagi, Amit; Dixit, V.P.
    In the present study, about 168 tissue samples were analyzed for the presence of endosulfan and chlorpyrifos residues and 74 for presence of monocrotophos using the standardized method. The residues were extracted by treating with acetonitirile followed by liquid-liquid partition with sodium sulfate solution (2.5%): dichloromethane. The extracts obtained after dehydration on sodium sulfate column were cleaned up by performing adsorption chromatography on alumina column. The detection and quantification of these residues was carried out with the help of High Performance Liquid Chromatography using Diode Array Detector. Five (7.14 %) out of 70 muscle, 2 (2.85 %) of 70 liver and none of 70 kidney tissues were detected positive for endosulfan α residues. Out of these none of the samples violated the prescribed limits given by CODEX. Only one (1.42 %) of muscle tissue sample out of 70 was detected positive for endosulfan β residues. Out of these also none of the samples violated the prescribed limits given by CODEX. Seven (10.00 %) of muscle tissue, 6 (8.57 %) of kidney and 2 (2.85 %) of liver were detected positive for endosulfan sulfate residues. Out of these total samples none of the samples violated the prescribed limits given by CODEX. Chlorpyrifos residues were detected in 5 (7.14 %) of 70 muscle samples, 4 (5.71 %) of 70 liver and none of kidney tissue was detected positive. None of these samples also violated the prescribed limits given by CODEX. All of the 121 samples analyzed for presence of monocrotophos were found negative.
  • ThesisItemOpen Access
    Detection and quantification of endosulfan and chlorpyrifos residues in buffalo meat, using high performance liquid chromatography
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2006-01) Pradeep Kumar; Singh, S.P.
    In the present study, methods for the extraction, cleanup, detection and quantification of endosulfan and chlorpyrifos residues from buffalo meat tissues (muscle and liver) were standardized. As many as 556 buffalo tissue (muscle and liver) samples collected from various locations of Uttaranchal and Bareilly of Uttar Pradesh were analyzed for the presence of endosulfan and chlorpyrifos residues. The endosulfan and chlorpyrifos residues were extracted by treating with acetonitirile followed by liquid-liquid partition with sodium sulfate solution (2.5%):dichloromethane. The extracts obtained were cleaned up by performing adsorption chromatography on alumina column. The detection and quantification of these residues was carried out with the help of High Performance Liquid Chromatography. Forty four (7.91%) out of 556(8.6% muscle and 7.08% liver) tissues were detected positive for endosulfan α residues. Out of these, 42 tissue samples (7.55% of the total samples) violated the prescribed limits given by CODEX. Twenty three (4.13%) of the total tissue samples (4.96% of 302 muscle and 3.14% of 254 liver) were detected positive for endosulfan β residues. Out of these, 21 tissue samples (3.77% of the total samples) were found to contain the residues above the MRL (CODEX). Fifty six (10.07%) of the total tissue samples (11.58% of 302 muscle and 8.26% of 254 liver) were detected positive for endosulfan sulfate residues. Out of these, 43 tissue samples (7.73% of the total samples) violated the limits prescribed by CODEX. The mean residual concentration estimated was 0.954960.04146, 2.930940.17633 and 0.574960.0464μg/g for endosulfan α, endosulfan β and endosulfan sulfate, respectively. Chlorpyrifos residues were detected in 41 (7.37%) of 556 tissue samples (5.96% of 302 muscle and 9.05% of liver) analyzed with the mean residual concentration of 0.28561000.02617μg/g. Of these 4 (0.71% of the total samples) tissue samples from Haldwani slaughter house violated the prescribed limits given by CODEX. However, 20 (3.59% of the total samples) tissue samples violated the Indian standard.
  • ThesisItemOpen Access
    Studies on the pesticide (Chlorpyrifos and Endosulphan) residues in water, milk and feed/fodder using high performance liquid chromatography
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2005-01) Karabasanavar, Nagappa; Singh, S.P.
    In the present study residual concentrations of chlorpyrifos and endosulphan residues in water (152), milk (170), feed (40) and fodder (25) samples collected from various locations of Tarai and Kumaon regions of Uttaranchal were determined. For extracting these residues from water C-18 cartridges were used, while liquid-liquid partition followed by alumina column chromatography was used for the clean up and the detection and quantification of these residues was undertaken with the help of HPLC using diode array detector. Chlorpyrifos residues were detected in 1.32 % of the samples with the mean residual concentration of 0.036 μg/ml, while 13.2 % samples showed the residues of total endosulphan with the mean residual concentration of 0.278, 0.212 and 0.276 μg/ml, respectively, for endosulphan α, endosulphan β and endosulphan sulphate; where, 1.32 % and 11.18 % samples violated the prescribed limit for chlorpyrifos and endosulphan, respectively. About 4.7 and 8.23 % of milk samples showed chlorpyrifos and total endosulphan residues, respectively, with the mean residual concentration of 0.092, 0.244, 0.566 and 0.265 μg/ml, respectively, for chlorpyrifos, endosulphan α, endosulphan β and endosulphan sulphate. Of the total 170 milk samples analyzed 8 (4.7 %) and 11 (6.47 %) samples respectively, were found to contain chlorpyrifos and endosulphan residues above the prescribed MRL. About 17.5 % of feed samples were positive for chlorpyrifos with mean residual concentration of 0.058 μg/g. On the other hand, 40 % samples were found positive for total endosulphan with the mean residual concentration of 0.402, 0.147 and 0.373 μg/g, respectively, for endosulphan α, endosulphan β and endosulphan sulphate; where in about 22.5 % samples contained residues above the prescribed limit. Out of 25 fodder samples analyzed, chlorpyrifos residues were present in 4 % of samples with mean residual concentration of 0.390 μg/g, while endosulphan α and endosulphan sulphate were found in 44 % of samples with the mean residual concentration of 0.225 μg/g and 0.248μg/g, respectively. None of the samples, however, contained the residues above the prescribed limit.
  • ThesisItemOpen Access
    Studies on non-typhoidal Salmonella isolates obtained from indigenous and exotic layers of an organized poultry farm
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-07) Nagpal, Aastha; Maansi
    The present study was undertaken to assess the prevalence, virulence characteristics and antimicrobial resistance at phenotypic and genotypic level of non-typhoidal Salmonella isolated from layer flocks of indigenous (Uttara fowl and Kadaknath) and exotic (RIR, White Leghorn and Australorp) breeds of an organized farm at Pantnagar. A total of 470 samples were collected from 5 layer breeds, Uttara fowl (n=220), Kadaknath (n=55), RIR (n=91), White Leghorn (n=55) and Australorp (n=49). The samples (n=470) comprised of poultry faeces (n=90), litter (n=75), feed (n=70), water (n=65), eggs (n=120) [egg surface (60) + egg content (60)] and caecal content (n=50) of dead birds.The overall prevalence of non-typhoidal Salmonella was 4.89% (23/470). Higher prevalence was observed in exotic breeds (8.21%, 16/195) than indigenous breeds (2.55%, 7/275). Among the different breeds, RIR showed the higher occurrence (17.58%, 16/91), followed by Uttara fowl (2.73%, 6/220) and Kadaknath (1.82%, 1/55). White Leghorn and Australorp did not reveal any presence of Salmonella. Of the total samples (n=470), the highest prevalence was observed in water samples (7/65, 10.77%), followed by poultry faeces (6/90, 6.67%), caecal content (3/50; 6.0%), litter (4/75, 5.33%), feed (2/70, 2.86%) and egg samples (1/120, 0.83%). Only one sample of egg surface rinse was positive (1/60, 1.67%) and none of the egg content samples (n=60) showed positive result. Serotyping revealed the presence of a single serovar viz. Salmonella Typhimurium (91.3%, 21/23), while two isolates (8.7%, 2/23) remained untypable. All 23 isolates were screened for the presence of 8 virulence genes by PCR. Majority of the isolates (22) carried sipA (95.65%) followed by sopB 17 (73.91%), sopE1 14 (60.87%), stn 13 (56.52%), fliC 11 (47.83%) and mgtC 7 (30.43%), spvC and gipA 3 each (13.04%). All 23 isolates when tested against 13 antimicrobials, showed highest resistance for Erythromycin 23 (100%) followed by Ampicillin 15 (65.22%), Nalidixic Acid 13 (56.52%), Ciprofloxacin 12 (52.17%), Cefazolin 9 (39.13%), Cefotaxime 8 (34.78%), Sulfisoxazole 7 (30.43%), Enrofloxacin 6 (26.09), Gatifloxacin 5 (21.74%), Cefoxitin and Tetracycline 4 each (17.39%), Levofloxacin 3 (13.04%) and Streptomycin 2 (8.70%). Phenotypic co-resistance against Ciprofloxacin and Cefotaxime was identified in 5 (5/23, 21.74%) isolates. Eighteen out of twenty-three isolates (78.26%) were multidrug resistant (MDR). Sixteen different antimicrobial resistance patterns were observed. Of these, common resistance patterns were CZ CTX E AMP, NA CIP LE GAT EX E AMP SF, S E, NA CIP CTX E AMP TE SF, E AMP, NA CIP CZ CX CTX E AMP and NA CIP CZ CX E AMP (all 2/23, 8.7%). The multiple antibiotic resistance (MAR) index was found in a range of 0.15-0.69. All phenotypically resistant (including intermediate) isolates were screened for 16 corresponding antimicrobial resistance genes. The most commonly occurring resistant gene was gyrA (23/23, 100%) followed by parC (22/23, 95.65%), aadA1 (2/4, 50%), sul1 (3/7, 42.86%), blaTEM (5/23, 21.74%) and qnrS (1/23, 4.35%) while blaPSE, blaCMY, aadA2, strA, strB, sul2, tetA, tetB, tetG and qnrA were not found in any of the isolates. None of the isolates exhibited Class1 integrons. Overall, our study detected low occurrence of NTS from the layer flocks of an organized farm. Co-resistance to clinically important antimicrobials, ciprofloxacin and cefotaxime observed in this study is a matter of concern that necessitates only deliberate use of antimicrobials in the poultry farms in the country.
  • ThesisItemOpen Access
    Isolation, characterization and prevalence of thermophilic Campylobacters in poultry farms and meat vendors using novel enrichment method
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2019-07) Bisht, Piyush; Upadhyay, A.K.
    Campylobacters are Gram negative helical bacilli belonging to the family Campylobacteraceae and are of major public health significance as they are one of the leading cause of food borne gastroenteritis worldwide. The present study was carried out to find a novel technique for isolation of Campylobacter spp. and and determine the prevalence of thermophilic Campylobacter spp. in poultry and their living environment at different poultry farms and meat vendors located in Kumaon region of Uttarakhand state. Most of the Campylobacter culture media described in the literature are supplemented with sheep or horse blood, therefore, this study examined the use of goat blood as potential substitutes for sheep blood in blood-supplemented bacteriologic media commonly used in clinical microbiology laboratories. A total of 381 samples comprising of 156 poultry caeca, 86 poultry faeces, 25 goat faeces, 10 sheep faeces, 24 meat swabs and 80 environmental samples viz.; water (n=32) and litter (n=48) were collected from three (n=3) poultry farms and meat shops (n=4) were analyzed, of which 49 samples showed characteristic colonies, either having a spreading or watery nature. They were typical Gram negative spiral rods and had characteristic cork screw motility. All the 49 Campylobacter isolates were confirmed using biochemical and molecular assays. In latex agglutination test, all the isolates produced characteristic agglutination. Genus-specific PCR amplification of 16SrRNA gene yielded expected product of 816 bp in all the isolates. In multiplex PCR assay conducted targeting lpxA gene for the identification of C. jejuni (331 bp) and C. coli (391bp) was used. According to research, the mean of the viable count of bacteria obtained from media supplemented with Goat blood was 0.921x108 c.f.u./ml and sheep blood was 0.936x108 c.f.u./ml. The Chi square analysis (ϗ2) was performed which proves the result to be non-significant showing similarities between the results obtained by both the media. Hence, the data indicates that goat blood can also be used as alternative and all the studies were carried on goat blood. The prevalence rate of thermophilic campylobacters sample was found to be 12.79% (11/86) in poultry faeces, 21.15% (33/156) in poultry caeca, 12.5% (3/24) in meat swabs and 4.16% (2/48) in litter from poultry farms. No thermophilic Campylobacter was isolated from goat faeces, sheep faeces and water samples from the poultry farms. Farmwise, the highest prevalence in poultry farms was detected in Anandpur (11.7%) followed by Haldwani (7.5%), and Pantnagar (5.3%). Among the chicken meat shops, highest prevalence was detected in Pantnagar (24%) followed by Haldwani (23.3%), Lalkuan (15%) and Nainital (6.6%). Out of the total of 49 thermophilic Campylobacter isolated, comprising of 36 C. jejuni (67.34%) and 13 C. coli (32.65%). As poultry serve as important source of Campylobacter, awareness among the poultry farmers and chicken meat retailers should be created to prevent the further transmission of this zoonotic agent.