Studies on the pesticide (Chlorpyrifos and Endosulphan) residues in water, milk and feed/fodder using high performance liquid chromatography
Loading...
Date
2005-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
In the present study residual concentrations of chlorpyrifos and endosulphan residues in water (152), milk (170), feed (40) and fodder (25) samples collected from various locations of Tarai and Kumaon regions of Uttaranchal were determined. For extracting these residues from water C-18 cartridges were used, while liquid-liquid partition followed by alumina column chromatography was used for the clean up and the detection and quantification of these residues was undertaken with the help of HPLC using diode array detector.
Chlorpyrifos residues were detected in 1.32 % of the samples with the mean residual concentration of 0.036 μg/ml, while 13.2 % samples showed the residues of total endosulphan with the mean residual concentration of 0.278, 0.212 and 0.276 μg/ml, respectively, for endosulphan α, endosulphan β and endosulphan sulphate; where, 1.32 % and 11.18 % samples violated the prescribed limit for chlorpyrifos and endosulphan, respectively. About 4.7 and 8.23 % of milk samples showed chlorpyrifos and total endosulphan residues, respectively, with the mean residual concentration of 0.092, 0.244, 0.566 and 0.265 μg/ml, respectively, for chlorpyrifos, endosulphan α, endosulphan β and endosulphan sulphate. Of the total 170 milk samples analyzed 8 (4.7 %) and 11 (6.47 %) samples respectively, were found to contain chlorpyrifos and endosulphan residues above the prescribed MRL.
About 17.5 % of feed samples were positive for chlorpyrifos with mean residual concentration of 0.058 μg/g. On the other hand, 40 % samples were found positive for total endosulphan with the mean residual concentration of 0.402, 0.147 and 0.373 μg/g, respectively, for endosulphan α, endosulphan β and endosulphan sulphate; where in about 22.5 % samples contained residues above the prescribed limit. Out of 25 fodder samples analyzed, chlorpyrifos residues were present in 4 % of samples with mean residual concentration of 0.390 μg/g, while endosulphan α and endosulphan sulphate were found in 44 % of samples with the mean residual concentration of 0.225 μg/g and 0.248μg/g, respectively. None of the samples, however, contained the residues above the prescribed limit.
Description
Keywords
null