Detection and quantification of endosulfan and chlorpyrifos residues in buffalo meat, using high performance liquid chromatography
Loading...
Date
2006-01
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
In the present study, methods for the extraction, cleanup, detection and quantification of endosulfan and chlorpyrifos residues from buffalo meat tissues (muscle and liver) were standardized. As many as 556 buffalo tissue (muscle and liver) samples collected from various locations of Uttaranchal and Bareilly of Uttar Pradesh were analyzed for the presence of endosulfan and chlorpyrifos residues. The endosulfan and chlorpyrifos residues were extracted by treating with acetonitirile followed by liquid-liquid partition with sodium sulfate solution (2.5%):dichloromethane. The extracts obtained were cleaned up by performing adsorption chromatography on alumina column. The detection and quantification of these residues was carried out with the help of High Performance Liquid Chromatography. Forty four (7.91%) out of 556(8.6% muscle and 7.08% liver) tissues were detected positive for endosulfan α residues. Out of these, 42 tissue samples (7.55% of the total samples) violated the prescribed limits given by CODEX. Twenty three (4.13%) of the total tissue samples (4.96% of 302 muscle and 3.14% of 254 liver) were detected positive for endosulfan β residues. Out of these, 21 tissue samples (3.77% of the total samples) were found to contain the residues above the MRL (CODEX). Fifty six (10.07%) of the total tissue samples (11.58% of 302 muscle and 8.26% of 254 liver) were detected positive for endosulfan sulfate residues. Out of these, 43 tissue samples (7.73% of the total samples) violated the limits prescribed by CODEX. The mean residual concentration estimated was 0.954960.04146, 2.930940.17633 and 0.574960.0464μg/g for endosulfan α, endosulfan β and endosulfan sulfate, respectively. Chlorpyrifos residues were detected in 41 (7.37%) of 556 tissue samples (5.96% of 302 muscle and 9.05% of liver) analyzed with the mean residual concentration of 0.28561000.02617μg/g. Of these 4 (0.71% of the total samples) tissue samples from Haldwani slaughter house violated the prescribed limits given by CODEX. However, 20 (3.59% of the total samples) tissue samples violated the Indian standard.
Description
Keywords
null