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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Disposition kinetics and tissue residue study of sulphadiazine-trimethoprim and amprolium in broiler poultry
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-07) Bisht, Preeti; Ahmad, A. H.
    The present study was undertaken to investigate the pharmacokinetics and tissue residue study of sulphadiazine-trimethoprim ,when given intramuscular (i.m) in a combination (20mg.kg-1 sulphadiazine and 4mg.kg-1 trimethoprim) @ 24.0mg.kg-1 and amprolium given orally @ 30.0mg.kg-1 in poultry following single and multiple (5) dose administration. In the present study Biotrim®, a combination of sulphadiazine-trimethoprim in the ratio of 5:1 (sulphadiazine 400mg and trimethoprim 80mg) was given @24.0mg.kg-1 b.w i.m as a single dose to nine birds and as a multiple dose to nine birds for 5 consecutive days. Amprolium 20% was used and given @30.0mg.kg-1 b.w orally as a single dose to nine birds and as a multiple dose to nine birds for 5 consecutive days. The concentration of sulphadiazine-trimethoprim and amprolium in plasma and tissue of these animals was analysed by HPLC. The initial peak plasma concentration of 24.06 and 1.27μg.ml-1 for sulphadiazine and trimethoprim was detected in poultry following single dose i.m. administration and 0.94μg.ml-1 for amprolium following single dose oral admiministration, respectively. The volume of distribution, clearance, mean area under curve (AUC) and elimination half- life calculated was 0.85L.kg-1, 0.12L.h-1.kg-1, 171.12h.μg.mL-1 and 4.73h and 2.64 L.kg-1,0.43 L.h-1.kg-1, 9.71 h.μg.mL-1and 4.53h for sulphadiazine (SDZ) and trimethoprim (TMP) respectively, following single dose i.m administration of SDZ/TMP combination. For amprolium, the volume of distribution, clearance, mean area under curve (AUC) and elimination half- life following single dose oral administration calculated was 21.941 L.kg-1,14.446 L.h-1.kg-1,2.172 h.μg.mL-1 and 1.027h, respectively. In multiple dose study, volume of distribution, clearance, mean area under curve and elimination half-life calculated for sulphadiazine was 0.81 L.kg-1, 0.11 L.h-1.kg-1,176.41 h.μg.ml-1 and 4.93h after first dose and 0.80 L.kg-1, 0.12L.kg-1, 176.32 h.μg.ml-1 and 4.87 h after last dose, respectively. For TMP volume of distribution, clearance, mean area under curve and elimination half-life calculated as 2.75L.kg-1, 0.49L.h- 1.kg-1,8.72h.μg.ml-1 and 4.22h, respectively, after first dose and 2.81L.kg-1,0.35L.h-1.kg-1,11.70h.μg.ml-1 and 5.73h, respectively after last. For amprolium volume of distribution, clearance, mean area under curve and elimination half-life calculated was 16.731L.kg-1, 14.145L.h-1.kg-1, 2.202h.μg.ml-1 and 0.833±0.061h, respectively after first dose, 0.893L.kg-1,12.647L.h-1.kg-1,2.540h.μg.ml-1and 16.361h, respectively after last dose. According to the results obtained in the pharmacokinetic study an individualized dosage regimen were calculated. An i.m. dosage regimen of sulphadiazine with a priming dose of 43.32 mg.kg-1 and a maintenance dose of 35.25 mg.kg-1 for every 12 hr were estimated to maintain the therapeutic concentration. An i.m. dosage regimen of trimethoprim with a priming dose of 10.0mg.kg-1 followed by a maintenance dose of 8.7 mg.kg-1 at 12 h interval is recommended. For amprolium an oral dosage regimen with a priming dose of 28.4mg.kg-1 followed by a maintenance dose of 28.3 mg.kg-1 recommended. Following single dose i.m administration highest residual concentration of sulphadiazine and trimethoprim were observed in kidney (0.28 μg.g-1 for SDZ and 0.69μg.g-1 for TMP) followed by liver at 24hrs post administration of SDZ/TMP combination. No drug residue was found after 72h of post administration of SDZ/TMP combination. In case of multiple dose i.m administration of SDZ/TMP combination, residue of SDZ and TMP were present in kidney, liver and muscle upto 96hr post administration with highest concentration in kidney (0.78μg.g-1 for sdz and1.19 μg.g-1 for tmp). For amprolium following single dose oral administration residue were found in liver, kidney, muscles with highest concentration found in liver (0.53 μg.g-1) at 24hr post administration. In case of multiple dose oral administration of amprolium, highest concentration found in liver (0.62μg.g-1) at 48hr post administration. Tissue residue were found in liver, kidney and muscles upto 96 hr post administration of amprolium.
  • ThesisItemOpen Access
    Disposition kinetics and tissue residue study of Sulphadiazine-trimethoprim and amprolium in broiler poultry
    (G. B. Pant University of Agriculture and Technology, Pantnagar, 2022-07) Bisht, Preeti; Ahmad, A.H.
    The present study was undertaken to investigate the pharmacokinetics and tissue residue study of sulphadiazine-trimethoprim ,when given intramuscular (i.m) in a combination (20mg.kg-1 sulphadiazine and 4mg.kg-1 trimethoprim) @ 24.0mg.kg-1 and amprolium given orally @ 30.0mg.kg-1 in poultry following single and multiple (5) dose administration. In the present study Biotrim®, a combination of sulphadiazine-trimethoprim in the ratio of 5:1 (sulphadiazine 400mg and trimethoprim 80mg) was given @24.0mg.kg-1 b.w i.m as a single dose to nine birds and as a multiple dose to nine birds for 5 consecutive days. Amprolium 20% was used and given @30.0mg.kg-1 b.w orally as a single dose to nine birds and as a multiple dose to nine birds for 5 consecutive days. The concentration of sulphadiazine-trimethoprim and amprolium in plasma and tissue of these animals was analysed by HPLC. The initial peak plasma concentration of 24.06 and 1.27μg.ml-1 for sulphadiazine and trimethoprim was detected in poultry following single dose i.m. administration and 0.94μg.ml-1 for amprolium following single dose oral admiministration, respectively. The volume of distribution, clearance, mean area under curve (AUC) and elimination half- life calculated was 0.85L.kg-1, 0.12L.h-1.kg-1, 171.12h.μg.mL-1 and 4.73h and 2.64 L.kg-1,0.43 L.h-1.kg-1, 9.71 h.μg.mL-1and 4.53h for sulphadiazine (SDZ) and trimethoprim (TMP) respectively, following single dose i.m administration of SDZ/TMP combination. For amprolium, the volume of distribution, clearance, mean area under curve (AUC) and elimination half- life following single dose oral administration calculated was 21.941 L.kg-1,14.446 L.h-1.kg-1,2.172 h.μg.mL-1 and 1.027h, respectively. In multiple dose study, volume of distribution, clearance, mean area under curve and elimination half-life calculated for sulphadiazine was 0.81 L.kg-1, 0.11 L.h-1.kg-1,176.41 h.μg.ml-1 and 4.93h after first dose and 0.80 L.kg-1, 0.12L.kg-1, 176.32 h.μg.ml-1 and 4.87 h after last dose, respectively. For TMP volume of distribution, clearance, mean area under curve and elimination half-life calculated as 2.75L.kg-1, 0.49L.h- 1.kg-1,8.72h.μg.ml-1 and 4.22h, respectively, after first dose and 2.81L.kg-1,0.35L.h-1.kg-1,11.70h.μg.ml-1 and 5.73h, respectively after last. For amprolium volume of distribution, clearance, mean area under curve and elimination half-life calculated was 16.731L.kg-1, 14.145L.h-1.kg-1, 2.202h.μg.ml-1 and 0.833±0.061h, respectively after first dose, 0.893L.kg-1,12.647L.h-1.kg-1,2.540h.μg.ml-1and 16.361h, respectively after last dose. According to the results obtained in the pharmacokinetic study an individualized dosage regimen were calculated. An i.m. dosage regimen of sulphadiazine with a priming dose of 43.32 mg.kg-1 and a maintenance dose of 35.25 mg.kg-1 for every 12 hr were estimated to maintain the therapeutic concentration. An i.m. dosage regimen of trimethoprim with a priming dose of 10.0mg.kg-1 followed by a maintenance dose of 8.7 mg.kg-1 at 12 h interval is recommended. For amprolium an oral dosage regimen with a priming dose of 28.4mg.kg-1 followed by a maintenance dose of 28.3 mg.kg-1 at 12 h interval is recommended. Following single dose i.m administration highest residual concentration of sulphadiazine and trimethoprim were observed in kidney (0.28 μg.g-1 for SDZ and 0.69μg.g-1 for TMP) followed by liver at 24hrs post administration of SDZ/TMP combination. No drug residue was found after 72h of post administration of SDZ/TMP combination. In case of multiple dose i.m administration of SDZ/TMP combination, residue of SDZ and TMP were present in kidney, liver and muscle upto 96hr post administration with highest concentration in kidney (0.78μg.g-1 for sdz and1.19 μg.g-1 for tmp). For amprolium following single dose oral administration residue were found in liver, kidney, muscles with highest concentration found in liver (0.53 μg.g-1) at 24hr post administration. In case of multiple dose oral administration of amprolium, highest concentration found in liver (0.62μg.g-1) at 48hr post administration. Tissue residue were found in liver, kidney and muscles upto 96 hr post administration of amprolium.
  • ThesisItemOpen Access
    Evaluation of therapeutic efficacy of Amaranthus hypochondriacus seed extract in fipronil intoxicated rats with special reference to In Silico prediction of squalene-CYP3A1 gene interaction
    (G.B. Pant University of Agriculture and Technology, Pantnagar, District Udham Singh Nagar, Uttarakhand. PIN - 263145, 2022-08) Verma, Manish Kumar; Singh, S.P.
    This study was designed to evaluate the protective efficacy of Amaranthus hypochondriacus seed extract (HSEAH) following its oral administration @ 100 mg/kg b. wt. alone and simultaneously with fipronil @ 24.25 mg/kg b. wt. orally for 90 days in rats by measuring haematobiochemical, oxidative, reproductive, histopathological examination, impact on gene expression of CYP3A1 and residue analysis parameters. Hydroethanolic extract of the seed of Amaranthus hypochondriacus was prepared for phytochemical analysis and in vitro evaluation of antioxidant potential was undertaken in-silico prediction of squalene-CYP3A1 gene interaction. Phytochemical analysis revealed the presence of various phytochemical like alkaloid, flavonoids, carbohydrates, proteins, glycosides, oils, phenolic compounds, terpenoids, saponins and tannins whereas the coumarins, resins and steroids were absent. Mineral estimation reveled presence of calcium, iron magnesium, zinc, and copper was present in higher concentration whereas lead, cobalt and nickel in trace amount. In-silico study of cross linking comparison between squalene and fipronil-CYP3A1 gene interaction was found to have high binding affinities for squalene than fipronil. In vitro antioxidant activity assessed by measuring NO radical scavenging, DPPH radical scavenging, reducing power activity, and metal chelating of Fe+2 activities which revealed the excellent antioxidant potential of extract. For evaluation of protective efficacy of HSEAH in fipronil intoxicated rats, twenty four male Wistar rats weighing 90-100 gm of 4-6 weeks of age were divided equally and randomly into four groups. Group I served as control, in group II fipronil @24.25 mg/kg b. wt. po, in group III HSEAH @ 100 mg/kg b wt. po, and in group IV HSEAH 100 mg/kg b. wt. plus fipronil @24.25 mg/kg b. wt. po., were given for 90 days. The reduction in body weight and organ weight significantly decreased in fipronil group. However, reduction in body weight was restored in HSEAH treated group IV. Fipronil exposure caused significant (P<0.05) reduction in Hb, PCV, MCV, MCH, MCHC, TLC and DLC as compared to control groups. A significant (P<0.05) decline in total protein, albumin and globulin was observed in group II as compared to control. Fipronil plus HSEAH treated group IV showed significant (P<0.05) amelioration in the level of total proteins as compared to group II and at par with normal values in control showing ameliorative effect of HSEAH. A significant (P<0.05) increase in AST, ALT, ALP, LDH, glucose, creatinine, BUN, cholesterol, triglycerides and LDL were significant decreased in fipronil treated group II, which were restored by HSEAH towards normalcy indicating amelioration of these parameters by HSEAH. A significant (P<0.05) decline in catalase, GSH and SOD and an increase in LPO in RBCs and tissues was observed in group II which, however, returned to normalcy following simultaneous administration of HSEAH in group IV after 90 days. Administration of HSEAH alone in group III significantly elevated the catalase, GSH and SOD activity as compared to control group I. Histopathological changes were observed in liver, kidney, lungs, spleen, heart, intestine, testis, brain and thyroid fipronil treated rats which were ameliorated by treatment with HSEAH after 90 days in rats. Gene expression study revealed that expression of CYP3A1 gene was upregulated in fipronil treated rats and down regulated in group IV treated with fipronil plus seed extract as compared to animals of control group I. A significantly (P<0.05) high sperm morphological abnormalities and decrease in sperm motility, sperm viability, and sperm density was observed group II rat whereas, treatment with HSEAH restored these parameters towards normal values. A comparison of tissue residue study in between fipronil alone and combination with HSEAH, detected significantly (P<0.05) higher fipronil residues in the tissues of group II in comparison with group IV rats indicating ameliorating efficacy of HSEAH. It is concluded from this study that the extract of HSEAH exhibited the antioxidant property. Administration of fipronil @24.25 mg/kg b.wt. po, produced haemotoxic, hepatotoxic, nephrotoxic, cardiotoxic, reproductive toxic and oxidative, which were ameliorated following simultaneous administration of Amaranthus hypochondriacus seed extract @ 100 mg/kg b. wt. po, for 90 days in rats.
  • ThesisItemOpen Access
    Evaluation of antioxidative and immunomodulatory potential of Trigonella foenum graecum against imidacloprid induced toxicity in rats
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-01) Naveen Kumar; Ahmad, A.H.
    The study was carried out to investigate the ameliorating potential of Trigonella foenum graecum in combating imidacloprid induced immunotoxicity and oxidative stress in adult male Wistar albino rats. Rats were divided into five groups of eight rats each. In group I, vehicle (olive oil), group II, imidacloprid @ 170 ppm, group III, aqueous extract of seeds of Trigonella foenum graecum alone @ 12000 ppm, group IV imidacloprid @ 170 ppm and diosgenin @ 2400 ppm, and group V, imidacloprid @ 170 ppm and aqueous extract of Trigonella foenum graecum @ 12000 ppm were administered daily with feed to the rats orally for 90 days.The results of qualitative phytochemical analysis revealed presence of alkaloids, saponins, flavonoids, phenolic compounds and carbohydrates. The DPPH and ABTS radical scavenging assays for aqueous extracts were also found to be highest in seed extract. The rate of gain in body weight was significantly decreased in imidacloprid group, however, in combination with fenugreek and diosgenin it was restored to normal level. Imidacloprid exposure caused significant increase in serum level of ALT, AST, ALP and creatinine activities as compared to all other groups. Treatment with imidacloprid significantly decreased the RBC, Hb, PCV values and was significantly lower than either of the treatment. Imidacloprid produced toxicity in the form of enhanced lipid peroxidation and reduced GSH, SOD and catalase levels. Trigonella foenum graecum was significantly effective in restoration of these parameters towards normal. ROS generation was significantly increased following exposure to imidacloprid as compared to control. Significant (p<0.05) improvement in these parameters were observed in groups where imidacloprid along with fenugreek and diosgenin was administered. Total immunoglobulin, immunoglobulin G and foot pad thickness were significantly increased in imidacloprid group in comparison to co-treatment groups of imidacloprid with fenugreek and diosgenin control group. Thepresent study summarizes that the aqueous extract of Trigonella foenum graecum seeds and diosgenin exert ameliorative effects on imidacloprid induced toxic effects which may attribute to their antioxidative and immunomodulatory properties.
  • ThesisItemOpen Access
    Toxicological evaluation of indoxacarb and its amelioration by Hedychium spicatum L. in WLH cockerels and HepG2 cell culture models
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2016-09) Choudhary, Govind Kumar; Singh, S.P.
    This research work was undertaken to assess the immunotoxicological potential of indoxacarb and its amelioration following administration of Hedychium spicatum rhizome powder (HSRP) @ 2000 ppm and 4000 ppm in feed for 16 weeks study in White Leghorn Cockerels by determining the hematobiochemical and antioxidant parameters. Phytochemical analysis revealed the presence of alkaloid, flavonoid, phenolics, glycoside, saponins, carbohydrates, proteins and amino acids, diterpine, resins, fat and oils, phytosterols and tannins. In vitro evaluation of antioxidant property of different extracts by DPPH, ABTS and nitric oxide free radical scavenging assay showed that the methanolic extract has minimum IC50 in comparison to other extracts. Methanolic extract contained maximum concentrations of flavonoid and phenolics and also possessed in vitro anthelmintic efficacy against Hemonchus contortus. Forty nine male white leghorn chicks were divided equally and randomly into seven groups viz. I, II, III, IV, V VI and VII. Group I served as control and other groups were fed medicated ration as indoxacarb @ 250 ppm in II, silymarin @ 250 ppm in III, silymarin @250 ppm + indoxacarb @ 250 ppm in IV, HSRP @ 4000 ppm in group V, HSRP @ 2000 ppm + indoxacarb @ 250 ppm in group VI and HSRP @ 4000 ppm + indoxacarb @ 250 ppm in group VII, respectively, for 16 weeks and parameters were recorded at 8 and 16 weeks interval. A significant (P<0.05) decline in body weight and body weight gain was measured in indoxacarb treated cockerels, whereas, HSRP and silymarin treatments revealed an improvement in body weight gain. A significant (P<0.05) reduction in Hb, PCV, TEC, TLC, ALC, AHC and lymphocyte % was observed in indoxacarb treated birds and a significant (P<0.05) improvement in Hb, PCV, TEC, TLC was observed after treatment with HSRP. A significant decline in total serum protein, albumin, globulin and glucose in indoxacarb treated cockerels while significant (P<0.05) improvement in serum protein and glucose by HSRP and silymarin medication. A significant (P<0.05) increase in triglycerides, cholesterol, total bilirubin, indirect bilirubin, creatinine, AST, ALT, ALP and GGT was observed in cockerels treated with indoxacarb whereas, a significant (P<0.05) reduction in these parameters by supplemention with HSRP in dose dependent manner. A significant (P<0.05) decline in catalase, SOD, GSH, GPx and GST activity in RBC whereas an increase in LPO in RBC was observed in group II, however, these parameters were returned to normalcy following simultaneous medication with HSRP in groups VI and VII after 16 weeks. A significant (P<0.05) increase in tissue LPO, GPx and GST whereas significant reduction in tissues GSH, CAT and SOD observed in cockerels of indoxacarb treated Group II, whereas, a significant (P<0.05) improvement was observed in these parameters by supplemention with HSRP. A significant (P<0.05) decrease in serum IgY, IL-2, INF-γ and LST, MFT and CDNB reactivity by measuring the thickness of skin was observed in indoxacarb treated group II and simultaneous feeding of HSRP showed the amelioration which was at par with control. The sperm motility and sperm count was decreased significantly (P<0.05) whereas % dead sperm was increased significantly in indoxacarb treated cockerels. Simultaneous feeding of HSRP produces the protective effects. The SOD and CAT gene expression was significantly down regulated in indoxacarb treated group in comparison to control and feeding of HSRP showed down regulation but less than the indoxacarb treated groups. Whereas GPx, IL-2 and CYP1A1 gene were upregulated significantly (P<0.05) in indoxacarb treated group I in comparison to control but simultaneous feeding of HSRP produces improvement in the expression of these genes. Histopathological and ultra structural changes in liver, kidneys, spleen, heart, brain and testes were observed in indoxacarb treated groups whereas treatment with HSRP ameliorated the lesion. It is concluded from this study that the extracts of Hedychium spicatum have potent antioxidant and anthelmintic properties, and also produced amelioration in PCM induced toxicity in HepG2 cell line model. The rhizome powder of Hedychium spicatum @ 2000 ppm and @ 4000 ppm produced significant amelioration in indoxacarb @ 250 ppm induced haemotoxic, hepatotoxic, nephrotoxic and oxidative effects and immunosuppression following administration for 16 weeks in WLH cockerels.
  • ThesisItemOpen Access
    Evaluation of protective effect of Erythrina variegata and Spondias pinnata in cadmium intoxicated rats and their antiviral activity against PPR and CSF virus in cell lines
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2014-07) Nrip Kishore Pankaj; Singh, S.P.
    This investigation was undertaken to evaluate ameliorative efficacy of the bark powder of E. variegata (EVBP) and Spondias pinnata (SPBP) against cadmium chloride induced toxic effects in a 90 days trial in rats. Hydroethanolic bark extract of E. variegata (EVBE) and S. pinnata (SPBE) were also evaluated for their antiviral activity against PPR and CSF viruses using vero and PK-15 cell lines and effects on smooth muscle activities of isolated rat ileum during the investigation. Forty two, six month old, albino wistar rats, weighing 170-200gm, were randomly and equally divided into seven groups. For 90 days trial, group I served as control. Groups II, III, IV, V, VI and VII were given cadmium chloride @100mg/L of drinking water. Group II served as negative control. Simultaneous treatment was given as silymarin@100mg/kg bwt in group III and EVBP and SPBP @ 1 and 2% in groups IV, V, VI and VII, respectively, in feed for 90 days. No clinical sign was observed except curved spine or lordosis in Cd treated group II. Haemato-biochemical and antioxidant parameters were examined at day 0, 30, 60 and 90 whereas LST and histopathological examination after 90 days. There was a significant (P<0.05) decline in haematological and immunological parameters Hb, PCV, TEC, TLC, DLC and LST in Cd treated groups whereas a significant elevation in these parameters was observed in treatment groups as compared to Cd treated group II. A significant increase in biochemical parameters AST, ALT, ALP: G ratio, BUN and creatinine, and reduction in total proteins, albumin and globulin were observed in Cd intoxicated rats after 90 days. SPBP, EVBP and silymarin treated groups showed significant (P<0.05) improvement in these biochemical parameters as compared to Cd-treated rats. Cd also significantly (P<0.05) suppressed antioxidant enzymes in comparison to control. SPBP and EVBP significantly ameliorated haematobiochemical, antioxidant and immunological parameters, however, did not reverse AST, ALT, ALP, BUN, creatinine, LPO, CAT, SOD and GST up to control level. The histopathological changes were characterized by haemorrhage, moderate to severe swelling of interstitial tissue and glomeruli along with coagulative necrosis of renal tubular epithelial cells in kidneys; severe congestion of central vein, large blood vessel with vacuolar degeneration of hepatocytes; severe congestion of alveolar capillaries and presence of mononuclear cells throughout the parenchyma of lungs; congestion and haemorrhage in heart and degeneration of cardiac muscles; and severe congestion of the blood vessel and moderate degeneration of neurons in brain. Histopathological changes were severe in Cd treated group while mild to moderate changes were found in EVBP and SPBP treatment groups. On electron microscopy, ultra structure of the renal cells showing disappearance of nucleolus, pyknosis and marginalisation of chromatin material at the periphery of the nucleus were evident in the Cd treated group II. Ultra structure details of hepatocyte were represented through vacuolation, marginalisation of chromatin material. A significant (P<0.05) elevation in the Cd residue was highest in kidney followed by liver and bones. EVBP and SPBP reduced residue level in liver and kidney but did not reveal any effect on bones. EVBE and SPBE did not reveal antiviral activity against PPR and CSF viruses in Vero and PK-15 cell lines. EVBE was found to have dose dependent spasmolytic activity on isolated ileum. It is concluded from this study that EVBP and SPBP @1 and 2 % in feed ameliorated the cadmium chloride, given @ 100 ppm in drinking water, induced haemotoxic, hepatotoxic, nephrotoxic, oxidative stress and immunosuppression following simultaneous administration for 90 days in rats. EVBE produced spasmolytic effect on isolated rat ileum However; antiviral activity against PPR and CSF viruses was not evident on in vitro studies in Vero and PK-15 cell lines