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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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Now showing 1 - 9 of 12
  • ThesisItemOpen Access
    DETECTION AND MOLECULAR CHARACTERIZATION OF ENTEROCOCCUS SPECIES FROM POULTRY MEAT
    (Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2021) Parmar Nilam Nareshbhai; Dr. M. N. Brahmbhatt
    The present study demonstrated the detection and molecular characterization of Enterococcus species in poultry meat. Enterococci are associated with food spoiling, especially animal origin food. The involvement of enterococci in human disease has raised concerns about food safety.
  • ThesisItemOpen Access
    EPIDEMIOLOGY, MOLECULAR CHARACTERIZATION AND COMPARISON OF DIAGNOSTIC TEST FOR RABIES VIRUS
    (DEPARTMENT OF VETERINARY PUBLIC HEALTH & EPIDEMIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2021) Jeetendrakumar H. Chaudhary; Dr. M. N. Brahmbhatt
    Rabies is one of the ancient recognized lethal encephalitis caused by the rabies lyssavirus affecting almost all kinds of mammals, all over the world. The organism is a neurotropic, enveloped, single stranded, negative sense RNA virus belonging to the family Rhabdoviridae and genus Lyssavirus under the order Mononegavirales. In India, majority of human rabies deaths are due to dog bites and therefore pre-exposure immunization of dogs is of utmost importance to prevent the spread of rabies to other animals. Correct and prompt diagnosis of rabies is also important for the postexposure prophylaxis/measures in human and animals. Study of rabies dynamics in the field is required to understand the genetic variations currently in circulation and their evolutionary adaptation and modifications thereby to help devise effective control measures.
  • ThesisItemOpen Access
    COMPARISON OF LOOP MEDIATED ISOTHERMAL AMPLIFICATION WITH POLYMERASE CHAIN REACTION FOR DETECTION OF SALMONELLA SPP. IN POULTRY MEAT
    (Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2020) Pargi Zalak Bharatkumar; Dr. J. B. Nayak
    The present study was conducted with aim to isolate and identify Salmonella species from poultry meat samples collected from retail meat markets in and around Anand, confirming Salmonella spp. isolates by polymerase chain reaction (PCR) and loop mediated isothermal amplification (LAMP), antimicrobial resistance pattern of isolates by disc diffusion method and comparing LAMP with PCR method by checking their sensitivity and specificity for detection of Salmonella species.
  • ThesisItemOpen Access
    “COMPARISON OF LOOP MEDIATED ISOTHERMAL AMPLIFICATION WITH POLYMERASE CHAIN REACTION FOR DETECTION OF STAPHYLOCOCCUS AUREUS & METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS IN CHEVON
    (Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2020) Sonali Thakur; Dr. M. N. Brahmbhatt
    The present study demonstrated the comparison of Polymerase Chain Reaction (PCR) with Loop Mediated Isothermal Amplification (LAMP) for detection of Staphylococcus aureus (S. aureus) and Methicillin Resistant Staphylococcus aureus (MRSA) in chevon. Total 150 raw chevon samples were collected from retail meat shops in and around Anand city. These samples were collected under aseptic conditions and then transferred to the laboratory on ice for further processing.
  • ThesisItemOpen Access
    ISOLATION AND MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING ESCHERICHIA COLI FROM MILK
    (Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2019) PAGHDAR DHARABEN M.; Dr. J. B. NAYAK
    The present study was carried out in the post graduate laboratory, Department of Veterinary Public Heath and Epidemiology, College of Veterinary Science and Animal Husbandary, A.A.U., Anand in order to isolation and molecular characterization of Extended Beta Lactamase Producing Escherichia coli from milk to study their cultural characteristic and to judge the hygienic level of different local dairy cattle farms in and around Anand, Gujarat. A total of 150 samples were collected from different dairy cattle farms located in Navali (25), Sai dairy farm (50), Chikhodara (25), Bedva (25) and Mogar (25). All the samples were first inoculated on MacConkey Agar (MCA) and isolates showing pink colonies (lactose fermenting) were further transferred to Eosin Methylene Blue Agar (EMB) for confirmation and greenish metallic sheen producing isolates were confirmed as E. coli isolates.
  • ThesisItemOpen Access
    ISOLATION AND MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING ESCHERICHIA COLI FROM CHICKEN
    (Department of Veterinary Public Health and Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand, 2019) Gida Harpalbhai; Dr. M. N. Brahmbhatt
    This study was undertaken in order to isolate and molecularly characterize extended spectrum beta lactamase producing Escherichia coli from chicken, to check their prevalence and to judge hygienic level of different local chicken butcher’s and retail market meat in and around anand. Total 150 chicken muscle samples were collected from chicken shops in and around Anand under aseptic precautions in sterile screw lid sample collector for further processing and microbiological analysis. Samples were collected aseptically in sterile test screw capped vials and immediately transferred to the laboratory for processing and bacteriological investigation.
  • ThesisItemOpen Access
    DETECTION OF TETRACYCLINE ANTIBIOTICS IN MILK OF ANAND DISTRICT
    (COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Mistry Urvish Pravinbhai; Dr. M. N. Brahmbhatt
    This study was undertaken with the objective of determining the overall presence of the Tetracycline antibiotic residues in raw and pasteurized milk samples collected from different localities of Anand district, Gujarat. Establishing sensitivity and specificity of immuno-photometric test and comparing the method with traditional chromatographic techniques for residue detection was the complementary objective. The research work was done at Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, AAU, Anand and Food Quality Testing Laboratory, College of Food Processing Technology and Bio-Energy, AAU, Anand.
  • ThesisItemOpen Access
    SEROPREVALENCE OF BRUCELLOSIS IN SMALL RUMINANTS AND HUMANS OF ANAND DISTRICT
    (DEPARTMENTOF VETERINARY PUBLIC HEALTH AND EPIDEMIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) RADHABEN R. PADHER; DR. J. B. NAYAK
    The present study was carried out at the post graduate laboratory, Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, A.A.U., Anand with the intention to determine the seroprevalence of brucellosis among small ruminants and human beings of Anand district, Gujarat employing RBPT, STAT and I-ELISA and compared the efficacy of these tests.
  • ThesisItemOpen Access
    ISOLATION, IDENTIFICATION AND DETECTION OF VIRULENCE FACTOR ASSOCIATED GENES OF CAMPYLOBACTER SPP. FROM BUFFALO MEAT
    (AAU, Anand, 2015) PATEL, SWATI N.; BRAHMBHATT, M. N.
    Campylobacter spp. has been recognized as one of the most frequent cause of gastrointestinal illness in humans throughout world. In recent years, the frequency of human enteritis caused by Campylobacter spp. has exceeded those caused by salmonella especially in developed countries. The association of zoonotic Campylobacters with chronic and life threatening complications such as Guillain- Barre syndrome, reactive arthritis, haemolytic uraemic syndrome, abortion, meningitis etc. which increases the public health concern of this pathogen worldwide. Campylobacter is normal inhabitant of intestinal tract of food animals and faulty handling, lack of sanitation at processing and improper storage has been considered as the major factor contaminating all types of animal foods. Inadequately cooked meat, unpasteurized milk and contaminated drinking water are the most common sources for epidemic and sporadic food borne cases. Therefore, the present study was undertaken with the objective to isolate identify and characterize the Campylobacter spp. from raw buffalo meat intended for the human consumption available in the retail meat market in Anand, Gujarat. A total 200 samples including 75 beef, 75 liver, 25 intestinal content and 25 water samples were collected aseptically and processed immediately after arrival. The samples were enriched into 25 ml of Blood free Campylobacter broth base containing CCDA selective supplement. Mixed properly and incubated at under microaerophilic condition (85% N2, 5% O2 and 10% CO2) at 42° C for 48 hours and further inoculation on blood free Campylobacter selective agar (mCCDA). The presumptive Campylobacter isolates were further confirmed at genus and species level by the series of biochemical tests and also confirmed by polymerase chain reaction assay. The confirmed isolates were subjected for the antibiotic susceptibility test using seven antibiotics, detection of virulence determinants by PCR. In the present study, 7 out of 200 (3.5%)) samples in which 2 out of 75 (2.67 %)) buffalo beef, 3 out of 75 (4 %) liver, 2 out of 25 (8 %>) intestinal content and no any water sample were found positive for the Campylobacter spp. In all the seven isolates the only identified species was Campylobacter jejuni. All the seven isolates of Campylobacter were further processed to study antibiotic resistance and sensitivity pattern against seven antibiotics by using agar disc diffusion technique. All Campylobacter isolates were resistant to Ciprofloxacin (100%)), where as 85.71% and 28.57% resistant to Nalidixic acid and Tetracycline, respectively. All the Campylobacter isolates were sensitive to Chloramphenicol, Erythromycin, Gentamicin and Strepyomycin sulphate (100%)) followed by Tetracycline (71.42%o) and Nalidixic acid (14.28%). All the isolates were also subjected for in vitro detection of five virulence gene encoding for viz., Flagellin gene (flaA), Campylobacter adherence gene (CadF), Invasion associated marker (iamA) gene. Flagellar synthesis and modification (flgR) and Cytolethal distending toxin Subunit B gene (cdtB) by polymerase chain reaction (PCR) using specific primers. The result indicated that all the seven isolates were found positive for CadF, CdtB and JlgR gene (100%) while six isolates were positive for flaA gene (85.71%) and three isolates were positive for iamA gene (42.85%). Therefore, the presence of Campylobacter in buffalo meat confirms that raw meat consumption could be a significant risk factor for human beings and there is need for routine study of this pathogen in buffalo meat to decrease the chances of infection with Campylobacter by consumption.