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Anand Agricultural University, Anand
Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur
AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.
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ThesisItem Open Access COMPARISON OF LOOP MEDIATED ISOTHERMAL AMPLIFICATION WITH POLYMERASE CHAIN REACTION FOR DETECTION OF SALMONELLA SPP. IN POULTRY MEAT(Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2020) Pargi Zalak Bharatkumar; Dr. J. B. NayakThe present study was conducted with aim to isolate and identify Salmonella species from poultry meat samples collected from retail meat markets in and around Anand, confirming Salmonella spp. isolates by polymerase chain reaction (PCR) and loop mediated isothermal amplification (LAMP), antimicrobial resistance pattern of isolates by disc diffusion method and comparing LAMP with PCR method by checking their sensitivity and specificity for detection of Salmonella species.ThesisItem Open Access “COMPARISON OF LOOP MEDIATED ISOTHERMAL AMPLIFICATION WITH POLYMERASE CHAIN REACTION FOR DETECTION OF STAPHYLOCOCCUS AUREUS & METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS IN CHEVON(Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2020) Sonali Thakur; Dr. M. N. BrahmbhattThe present study demonstrated the comparison of Polymerase Chain Reaction (PCR) with Loop Mediated Isothermal Amplification (LAMP) for detection of Staphylococcus aureus (S. aureus) and Methicillin Resistant Staphylococcus aureus (MRSA) in chevon. Total 150 raw chevon samples were collected from retail meat shops in and around Anand city. These samples were collected under aseptic conditions and then transferred to the laboratory on ice for further processing.ThesisItem Open Access ISOLATION AND MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING ESCHERICHIA COLI FROM MILK(Department of Veterinary Public Health & Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2019) PAGHDAR DHARABEN M.; Dr. J. B. NAYAKThe present study was carried out in the post graduate laboratory, Department of Veterinary Public Heath and Epidemiology, College of Veterinary Science and Animal Husbandary, A.A.U., Anand in order to isolation and molecular characterization of Extended Beta Lactamase Producing Escherichia coli from milk to study their cultural characteristic and to judge the hygienic level of different local dairy cattle farms in and around Anand, Gujarat. A total of 150 samples were collected from different dairy cattle farms located in Navali (25), Sai dairy farm (50), Chikhodara (25), Bedva (25) and Mogar (25). All the samples were first inoculated on MacConkey Agar (MCA) and isolates showing pink colonies (lactose fermenting) were further transferred to Eosin Methylene Blue Agar (EMB) for confirmation and greenish metallic sheen producing isolates were confirmed as E. coli isolates.ThesisItem Open Access ISOLATION AND MOLECULAR CHARACTERIZATION OF EXTENDED SPECTRUM BETA LACTAMASE PRODUCING ESCHERICHIA COLI FROM CHICKEN(Department of Veterinary Public Health and Epidemiology College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand, 2019) Gida Harpalbhai; Dr. M. N. BrahmbhattThis study was undertaken in order to isolate and molecularly characterize extended spectrum beta lactamase producing Escherichia coli from chicken, to check their prevalence and to judge hygienic level of different local chicken butcher’s and retail market meat in and around anand. Total 150 chicken muscle samples were collected from chicken shops in and around Anand under aseptic precautions in sterile screw lid sample collector for further processing and microbiological analysis. Samples were collected aseptically in sterile test screw capped vials and immediately transferred to the laboratory for processing and bacteriological investigation.ThesisItem Open Access DETECTION OF TETRACYCLINE ANTIBIOTICS IN MILK OF ANAND DISTRICT(COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Mistry Urvish Pravinbhai; Dr. M. N. BrahmbhattThis study was undertaken with the objective of determining the overall presence of the Tetracycline antibiotic residues in raw and pasteurized milk samples collected from different localities of Anand district, Gujarat. Establishing sensitivity and specificity of immuno-photometric test and comparing the method with traditional chromatographic techniques for residue detection was the complementary objective. The research work was done at Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, AAU, Anand and Food Quality Testing Laboratory, College of Food Processing Technology and Bio-Energy, AAU, Anand.ThesisItem Open Access SEROPREVALENCE OF BRUCELLOSIS IN SMALL RUMINANTS AND HUMANS OF ANAND DISTRICT(DEPARTMENTOF VETERINARY PUBLIC HEALTH AND EPIDEMIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) RADHABEN R. PADHER; DR. J. B. NAYAKThe present study was carried out at the post graduate laboratory, Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, A.A.U., Anand with the intention to determine the seroprevalence of brucellosis among small ruminants and human beings of Anand district, Gujarat employing RBPT, STAT and I-ELISA and compared the efficacy of these tests.ThesisItem Open Access ISOLATION AND CHARACTERIZATION OF AEROMONAS SPECIES FROM POULTRY MEAT(AAU, Anand, 2009) SMITA; Brahmbhatt, M. N.The aim of the present study was to isolate, identify and characterize Aeromonas species from poultry meat. A total of 120 samples were processed for estimating prevalence oi Aeromonas spp. Out of those 120 samples, 66 isolates oi Aeromonas were identified. On the basis of biochemical characterization 47 isolates of A. sobria, 11 isolates of A. hydrophila and 8 isolates of A. caviae were detected. When the source wise study of Aeromonas was conducted it was found that maximum number of Aeromonas isolates were recovered from heart (87.5 per cent) followed by liver (66.66 per cent), thigh muscle and chest muscle (40 per cent each). When different selective culture media were evaluated for isolation of Aeromonas spp. from poultry meat it was found that percent recovery of Aeromonas isolates were more from Ampicillin Dextrin Agar (89.39 per cent), followed by Aeromonas Starch DNAse agar (68.18 per cent) and Aeromonas isolation media (18.18 per cent). Specificity of PCR assay for detection of A. hydrophila and A. sobria was performed by testing against different gram positive and gram negative organisms.Primers were found to be specific for A. hydrophila and A. sobria. All the isolates of A. sobria (47) and A. hydrophila (11) which were identified on the basis of biochemical characterization were subjected to PCR and confirmed. All 66 Aeromonas isolates were tested for presence of aerolysin, haemolysin and enterotoxin gene. None of the isolate showed presence of aerolysin and enterotoxin while overall prevalence of haemolysin gene was 78.78 per cent. In A. hydrophila, 54.54 per cent; A. caviae, 37.5 per cent and in A. sobria, 91.48 per cent isolates were found to possess haemolysin gene. All the isolates of Aeromonas were subjected to antimicrobial drug sensitivity test against gentamicin, chloramphenicol, ciprofloxacin, kanamycin, bacitracin, rifampicin, tetracycline and erythromycin. Maximum sensitivity pattern was recorded with chloramphenicol (86.36per cent), gentamicin (81.82 per cent), ciprofloxacin (60.61 per cent), kanamycin (34.85 per cent), bacitracin (25.76 per cent), tetracycline (16.67 per cent), rifampicin (15.15 per cent) and erythromycin (9.09 per cent). The resistance pattern of Aeromonas isolated from chicken meat to various antibiotics was observed as bacitracin (74.24 per cent) followed by rifampicin (71.21 per cent), kanamycin (65.15 per cent), erythromycin (53.03 per cent), tetracycline (33.33 per cent), ciprofloxacin (22.73 per cent) and gentamicin (18.18 per cent). It was concluded that overall prevalence of Aeromonas spp. in poultry meat was 55 per cent which is a matter of concern from public health point of view and needs proper attention. The overall prevalence of 78.78 per cent of Aeromonas isolates showing presence of haemolysin gene (Virulence gene) in poultry meat is also a matter of concern and this study reveals that poultry meat could be a potential threat to public health. Hence, more attention for implementation of HACCP concept from food safety point of view is required. Antibiotic sensitivity pattern of Aeromonas isolates revealed that chloramphenicol was found to be most effective drug (86.36per cent) followed by gentamicin (81.82 per cent), ciprofloxacin (60.61 per cent), kanamycin (34.85 per cent), bacitracin (25.76 per cent), tetracycline (16.67 per cent), rifampicin (15.15 per cent) and erythromycin (9.09 per cent).ThesisItem Open Access EPIDEMIOLOGICAL STUDIES ON CANINE ZOONOTIC HELMINTHS WITH PARTICULAR REFERENCE TO TOXOCARA CANIS(AAU, Anand, 1999) Brahmbhatt, M. N.; Pal, MahendraHelminthological examination of 614 faecal samples from pet dogs attending the O.P.D. of the Clinics of the Veterinary College, Gujarat Agricultural University Campus, Anand was undertaken for a period of 12 months from November 1997 to October 1998. In addition, 115 faecal samples collected from stray dogs in and around the Anand city of Gujarat were also investigated for various helminths. The overall helminth prevalence rate observed in pet dogs was 34.53 per cent with 32.23 per cent in male and 37.85 per cent in female animals. The overall prevalence of helminths in the dogs between the age of 13 to 24 months was significantly higher. No significant difference in the prevalence of helminthic infection in male and female animals within each age group would be recorded. Stray dogs showed the overall prevalence of 41.74 per cent. Monthwise prevalence of parasitic infection in stray dog and pet dog population revealed high prevalence in the months of winter season. The examination of faecal samples from pet as well as stray dogs revealed Ancylostoma caninum (24.59 and 26,96%) and Toxocara canis (9.61 and 19.13%), Dipylidium caninum (2.44 and 3.48%), Toxascahs leonina (1.79 and 2.61%), Uncinaria stetiocephala (1.98 and 2.61%), Echinococais spp. (0.65 and 3.48%), Strongyloides stercoralis (0.65 and 1.74%)Diphyllobothrium latum (0.49 and 1.74%), Trichuris vulpis (0.0 and 1.74%) and Spirometra spp. (0.0 and 0.87%)). The infection rate was found almost similar in male and female animals for all the helminths Agcwise distribution of individual helminth did not reveal any definite trend. Monthwise and seasonal distribution of various helminths showed fairly high prevalence for each helminth during winter season followed by summer and monsoon. Single or multiple helminthic infection in pet and stray dog indicated single helminth infection in 28,34 and 21.74 per cent, two species infection in 5.86 and 17.39 per cent and three species infection in only 0.33 and 2.61 per cent samples respectively. Among the various sites of collection, the highest overall prevalence of helminths in stray dogs was observed in the samples from road sides. Epidemiological study of T.canis in pet and stray dog population showed the prevalence of 9.61 and 19.13 per cent, respectively. Monthwise and seasonal prevalence of T.cams in both the canine population was observed higher during the months of November-February and lowest in October. No significant difference was observed in sexwise prevalence of T.cams in pet dogs, and sitewise prevalence of T.canis in stray dogs, however, showed higher prevalence in the samples obtained from playgrounds. The prevalence of T.canis in 504 soil samples collected from various localities revealed overall prevalence rate of 26.39 per cent. Among the various sites of collection, highest prevalence was observed in the soil samples from slum area (45.83%) followed by playgrounds (37.50%)), rural area (27.78%), gardens/public places (26.39%), school compounds (22.22%), liuman dwellings (13 18%) and lowest in samples from road sides (11.11%). Monthwise prevalence of T.canis in soil samples indicated highest prevalence in December (36.09%) and lowest in October (11.90%) and seasonal prevalence showed higher values in winter season (29.17%). Haematological studies following the experimental infection with T.canis in laboratory mice revealed elevated eosinophilic count with peak after 2-3 weeks of postinfection. Higher values were recorded in mice with booster infection. Moderate leucocytosis and slight neutrophilia were observed throughout the study. Gradual decrease of haemoglobin and reduction in PCV was noticed after 24 day post-infection. Histopathological changes in the experimentally inoculated mice were noticed in the liver, muscle, lung, brain, kidney and spleen. The liver, lungs and kidneys showed fatty changes, congestion, cellular infilteration, granuloma formation and necrosis. Meningitis, focal liquefactive necrosis and gliosis were observed in the brain. No significant histopathological lesions were noticed except mild degenerative changes in the cardiac muscles. Seroprevalence study in human serum samples failed to demonstrate antibodies against T.canis in the specified group of persons such as staff members and students of Veterinary College. However, 8.57 per cent serum samples from 70 children showed positive reaction when tested by agar gel precipitation technique. Detailed clinical examination of positive cases showed leucocytosis, eosinophilia, fever, coughing, pneumonia and dyspnoea. Epidemiological investigation indicated that majority of positive cases had the habit of eating soil and history of contact with dogs. Blood smear examination of 159 children for the presence of eosinophilia along with the epidemiological information about the patient collected in a prescribed questionaire format revealed higher percentage of moderate, marked and severe eosinophilia in 0 to 5 year age group, association of dog ownership, poor socio-economic class, geophagia, habit of playing in soil, open school compound, improper personal hygiene and illiterate group with practically no educational background. It was concluded that the overall helminth infection was more prevalent in stray dog population as compared to pet dog population. No significant difference was observed in sex but the significant difference was observed in the various age group. Significantly higher prevalence was noticed in winter season. The prevalence for individual helminth was observed higher in stray dogs with more number of helminth. Single infection was found higher in pet dogs while mixed infection was noticed higher in stray dogs. Prevalence of T.canis was recorded higher in stray dogs as compared to pet dogs. Age group between birth to 4 months was more frequently affected with T.canis. Examination of soil samples for T.canis showed prevalence rate of 26.39 per cent with highest prevalence in soil samples from slum area. Experimental infection in mice is characterized by eosinophilia, and histopathological changes such as congestion, degeneration, cellular infiltration, moderate to marked fatty changes, necrosis and granuloma formation were observed in various organs. Seroprevalence study showed 8.57 per cent prevalence of T.canis antibodies in children who had contact with dog. On blood smear examination, eosinophilia was found as the constant feature in 152 children.ThesisItem Open Access MEAT SPECIATION BY MOLECULAR AND SEROLOGICAL TECHNIQUES(AAU, Anand, 2002) Thumbar, J. M.; BRAHMBHATT, M. N.Among several methods of meat speciation, method based on DNA especially Polymerase Chain Reaction (PCR) provide potentially more information than others. The present study was under taken for meat speciation by molecular and serological techniques. Meat samples from meat species (cattle, buffalo, sheep, goat, pig and chicken) were utilized for molecular analysis. Genomic DNA was isolated from eight meat samples of each species as per method described by Sambrook et al. (1989) with some modifications. Actin multigene family was amplified by PCR using a pair of family specific primers (forward primer-5' CCT ACA ACA GCA TCA TGA AGT G 3'and reverse primer-5' GCT GAT CCA CAT CTG CTG GAA G 3'). PCR cycling protocol included to initial denaturation at 95 °C for 5 minutes then followed by 30 cycles of 95°C for 1 minute, 48°C for 1 minute, 72°C for 1 minutes and final extension at 72°C for 1 minute, 48°C for 1 minute, 72°C for 1 minutes and final extension at 72°C for 5 minutes. PCR products were resolved by agarose gel electrophoresis it produced a characteristic band pattern for each species. PCR band pattern generated for cattle included one major band of 0.328 kb. The PCR band pattern for buffalo included two bands of 0.328 and 0.242 kb. Sheep and goat produced identical band patterns having two bands of 0.328 and 0.242 kb and one faint band of 0.765 kb. Pig also revealed pattern identical to buffalo. A band of approximately 0.330 kb size was common in all livestock species. Chicken exhibited a characteristic pattern with six bands, three bands of high intensity (approximately 0.365, 0.334 and 0.242 kb) and three faint bands (approximately 0.547, 0.480 and 0.113 kb). Out of six species explored, four were found to have characteristic PCR band pattern. Thus, PCR was considered to be a potential technique for meat species identification and speciation. Actin gene band patterns were identical in both the sexes for all the species studies. PCR was also found to be consistent and effective tool as it remained same for heat treated and putrified (unpreserved) of meat. An agar gel precipitation technique was successfully used for identification of meat species, cross-reaction study and detection of adulteration level in meat. Hyper immune sera were raised in rabbits by intramuscular injection of meat extract. Anti buffalo meat sera were cross-reacted to cattle, sheep and goat meat extract. At same time anti cattle meat sera were also found to be crossreacted with buffalo, sheep and goat meat extract. Species specific sera were made by absorption technique. These sera were used to detect level of adulteration. By AGPT, up to 10 % level of adulteration between buffalo or cattle meat with sheep and goat meat was successfully detected. Counter immunoelectrophoresis technique was also successfully used to detect the meat species. This method was found to be rapid as compare to the AGPT.
