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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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20044
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Subject: Veterinary Pharmacology and Toxicology × End date: 2004 × Start date: 2004 × Type: Thesis ×
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Now showing 1 - 4 of 4
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    ThesisItemOpen Access
    Comparative Evaluation of Dexamethasone Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicks
    (AAU, Anand, 2004) KALIA, ANIL KUMAR; Sarvaiya, J. G.
    The present work was planned to study induction of CYP3A and CYP2HI genes by Reverse Transcriptase polymerase chain reaction (RT-PCR) and Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Legiiorn chicles. Total RNA was extracted from the liver tissue samples using Tri Reagent based method. The quantity of extracted RNA was assessed spectrophotometrically at 260/280nm, and it ranged from 1.7 to 2.0 OD suggesting good quantity of RNA extraction. The quality of extracted RNA was checked by 1% formaldehyde agarose gel electrophoresis and it showed bands at 28s, 18s and 5s rRNA subunits suggesting good integrity of RNA. First strand cDNA was synthesized using one step RT-PCR Kit. The PCR was performed and the product was subjected to agarose gel electrophoresis which yielded targeted amplification of 1107 bp, 1567 bp and 486 bp amplicon for CYP3A, CYP2HI and p-actin genes, respectively. β-actin (house keeping gene) was used as an internal control for normalization of CYP3A and CYP2H1 gene transcripts. Quantitative RT-PCR was done to quantify gene expression level of CYP3A and CYP2H1 genes. Four end points were selected for sample dropping at 26th, 31st 36rd and 41st cycles to perform Quantitative RT-PCR. The quantity of expressed genes was detected by Gene tool software using 1 kb DNA ladder having concentration of 7.1 ng/0.5 μl at 500 bp as reference. Relative expression ratio of CYP3A and CYP2H1 genes was calculated by Relative Expression Software Tool (REST), It was found that CYP3A is up regulated by a factor of 1.271 and 1.2 in Bantam and White Leghorn chicks, respectively and down regulated by a factor of 11.385 in Bantamized White Leghorn chicks. In Bantamized White Leghorn and White Leghorn chicks CYP2H1 gene was down regulated by factor 1.68 and 1.3 respectively, but up regulated by a factor of 1.126 in case of Bantam chicks. The PCR efficiency ranged from 1.4 to 1.9, 1.36 to 1.8 and 1.36 to 4.4 for CYP3A, CYP2H1 and P-actin genes, respectively in Bantam, Bantamized White leghorn and White Leghorn chicks.
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    ThesisItemOpen Access
    TOXICOLOGICAL AND IMMUNOLOGICAL STUDIES OF SUB - ACUTE EXPOSURE OF COCKERELS TO IMIDACLOPRID AND QUINALPHOS
    (AAU, Anand, 2004) Siddiqui, M. Amir.; Thaker, A. M.
    The present study was conducted in 8 to 10 week old WLH cockerels, which were vaccinated with Fl vaccine of ranikhet disease at day old age. Approximate lethal medium dose (ALD50) of quinalphos (diethyl, 2-quinoxalyIphosphorothionate) and imidacloprid (1-{(6-chloro-3-pyridinylmethyl}-N-nitro-2-imadazolidinimine) was found to be 2.5 mg and 50 mg per kg body weight, respectively. l/50th and l/25th of ALD50 of both the insecticide were selected. In different groups of birds, daily oral administration of 50 and 100 ug per kg body weight of quinalphos and 1 and 2 mg per kg body weight of imidacloprid, suspended in 1 ml of groundnut oil, was carried out for 28 days. Before the start of administration of either insecticide or groundnut oil (control group) all the birds were vaccinated with Newcastle Disease Vaccine (R2B). All the birds were monitored for any observable toxic symptoms throughout the experimental period and they were also weighed weekly to monitor body weight gain. The blood samples were collected from wing vein at weekly interval and were analyzed for hematological, biochemical and immunological parameters. No observable symptoms were noticed throughout the investigation period. In first week of treatment, there was no significant change in the mean body weight of quinalphos and imidacloprid treated birds as compared to control. However, in the subsequent week there was a reduction in the body weight of both the insecticides treated birds. Both the insecticides produced hyperglycemia but did not affect total plasma cholesterol, packed cell volume and hemoglobin level. Initially after 7 days of treatment, plasma acetylcholenestrase were significantly decreased in quinalphos treated birds and was remained decreased until the end of experiment. Imidacloprid did not show any sign of acetylcholenestrase inhibition. Plasma transaminases viz. serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT) were increased in all the insecticide treated groups which was significant after 14 days of treatment. Total protein was decreased in all the insecticide treated birds after 14 days of treatment where as total globulin was decreased only in quinalphos treated groups. Antibody titre against NDV vaccine was significantly decreased in both of the insecticide treated group at first week of treatment, but beyond 14 days of treatment, antibody titre level did not show any significant reduction.
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    ThesisItemOpen Access
    STUDIES ON CEFTRIAXONE PHARMACOKINETICS FOLLOWING INTRAVENOUS AND INTRAMUSCULAR ADMINISTRATION IN BUFFALO CALVES
    (Anand Agricultural University, Anand, 2004) P.V.Gohil; Dr. A. M. Thaker
    Ceftriaxone, a broad spectrum semisynthetic third generation cephalosporin has excellent activity against gram negative bacteria as well as wide range of gram positive bacteria and some anaerobic bacteria, including enterobacteriaceae and many strain of pseudomonas aeruginosa. The present study was conducted to determine the pharmacokinetics of ceftriaxone after single dose intravenous and intramuscular administration in Surti buffalo calves. The ceftriaxone concentration in plasma was determined by High Performance Liquid Chromatography (HPLC).
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    ThesisItemOpen Access
    Comparative Evaluation of Dexamethasone Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicks
    (Anand Agricultural University, Anand, 2004) ANIL KUMAR KALIA; Dr. J.G. Sarvaiya
    The present work was planned to study induction of CYP3A and CYP2H1 genes by Reverse Transcriptase polymerase chain reaction (RT-PCR) and Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks. Total RNA was extracted from the liver tissue samples using Tri Reagent® based method. The quantity of extracted RNA was assessed spectrophotometrically at 260/280nm, and it ranged from 1.7 to 2.0 OD suggesting good quantity of RNA extraction. The quality of extracted RNA was checked by 1% formaldehyde agarose gel electrophoresis and it showed bands at 28s, 18s and 5s rRNA subunits suggesting good integrity of RNA. First strand cDNA was synthesized using one step RT–PCR Kit.