Comparative Evaluation of Dexamethasone Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicks
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Date
2004
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Anand Agricultural University, Anand
Abstract
The present work was planned to study induction of CYP3A and CYP2H1
genes by Reverse Transcriptase polymerase chain reaction (RT-PCR) and Quantitative
RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks. Total
RNA was extracted from the liver tissue samples using Tri Reagent® based method.
The quantity of extracted RNA was assessed spectrophotometrically at 260/280nm,
and it ranged from 1.7 to 2.0 OD suggesting good quantity of RNA extraction. The
quality of extracted RNA was checked by 1% formaldehyde agarose gel
electrophoresis and it showed bands at 28s, 18s and 5s rRNA subunits suggesting
good integrity of RNA. First strand cDNA was synthesized using one step RT–PCR
Kit.
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Keywords
Veterinary Pharmacology, Study