Comparative Evaluation of Dexamethasone Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicks

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dc.contributor.advisorDr. J.G. Sarvaiya
dc.contributor.authorANIL KUMAR KALIA
dc.date.accessioned2017-10-12T09:18:17Z
dc.date.available2017-10-12T09:18:17Z
dc.date.issued2004
dc.description.abstractThe present work was planned to study induction of CYP3A and CYP2H1 genes by Reverse Transcriptase polymerase chain reaction (RT-PCR) and Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks. Total RNA was extracted from the liver tissue samples using Tri Reagent® based method. The quantity of extracted RNA was assessed spectrophotometrically at 260/280nm, and it ranged from 1.7 to 2.0 OD suggesting good quantity of RNA extraction. The quality of extracted RNA was checked by 1% formaldehyde agarose gel electrophoresis and it showed bands at 28s, 18s and 5s rRNA subunits suggesting good integrity of RNA. First strand cDNA was synthesized using one step RT–PCR Kit.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810032982
dc.keywordsCOMPARATIVE EVALUATION OF DEXAMETHASONE,INDUCED CYP3A AND CYP2H1 GENE EXPRESSION BY QUANTITATIVE RT-PCR IN BANTAM, BANTAMIZED WHITE LEGHORN AND WHITE LEGHORN CHICKSen_US
dc.language.isoenen_US
dc.publisherAnand Agricultural University, Ananden_US
dc.research.problemComparative Evaluation of Dexamethasone Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicksen_US
dc.subVeterinary Pharmacology and Toxicologyen_US
dc.subjectVeterinary Pharmacologyen_US
dc.subjectStudyen_US
dc.themeCOMPARATIVE EVALUATION OF DEXAMETHASONEen_US
dc.these.typeM.V.Sc.en_US
dc.titleComparative Evaluation of Dexamethasone Induced CYP3A and CYP2H1 Gene Expression by Quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn Chicksen_US
dc.typeThesisen_US
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