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  • ThesisItemOpen Access
    MOHAMMED ARSHAD
    (KVAFSU,BIDAR, 2019) MOHAMMED ARSHAD
    Zoonotic aspects of mycobacteria transmitted through cattle, wild birds and associated environments underscores a serious public health threat. Infections caused by members of MAC depends on many factors.Infections caused by the zoonotic members of the MAC are often neglected with respect to prevalence and animal and human health risks. A cross sectional study was conducted in Shivamogga region of Karnataka by screening a total of 318 samples (cattle-100, wild birds – 100, associated environments- 118) by the use of five target multiplex PCR and nested multiplex PCR for the detection of MAC and MTBC respectively. When screened for the presence of Mycobacterium species, prevalence of 11 % and 20 % were obtained from cattle and associated environments respectively. Wild birds showed a prevalence of 6.73 % and the associated environments had a prevalence of 18.18 %. Prevalence of MAC in cattle and associated environments were found to be 7 % and 4.28 % respectively. Wild birds had a prevalence of 2.88 % and the associated environments showed a prevalence of 2.27 % for MAC. However, prevalence of Mycobacterium avium subspecies paratuberculosis (Map) in cattle and associated environments was same as that of MAC .None of the samples showed presence of MTBC when detected by a nested multiplex PCR. The study concluded that, mycobacteria are widespread organisms which can be detected by molecular tools like PCR. It also emphasized the need to include non tuberculous mycobacteria while designing the studies related to Mycobacterium spp as they significantly contribute to zoonotic infections. Keywords: Mycobacterium species, MAC, Cattle, wild birds, five target multiplex PCR, nested multiplex PCR.
  • ThesisItemOpen Access
    EPIDEMIOLOGY OF ZOONOTIC ESCHERICHIA COLI IN BOVINES IN SOUTHERN TRANSITION ZONE OF KARNATAKA
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR- 585401, 2019) KARADI MANJULA
  • ThesisItemOpen Access
    EPIDEMIOLOGY OF BRUCELLOSIS IN SMAKK RUMINANTS IN CENTRAL DRY ZONE
    (KARNATAKA VETERINARY, ANIMAL AND FOISHERIE'S SCIENCES UNIVERSITY, BIDAR, 2018-08) SHASHIDHAR SOPPIMATA; MADHAVPRASAD C B
  • ThesisItemOpen Access
    STUDIES ON CERTAIN QUALITY PARAMETERS AND PREVALENCE OF PATHOGENS OF PUBLIC HEALTH SIGNIFICANCE IN RAW MILK OF FARM ANIMALS
    (KARNATAKA VETERINARY, ANIMAL AND FOISHERIE'S SCIENCES UNIVERSITY, BIDAR, 2017-08) TARINI, N. K.; TARINI, N. K.; Dr. MADHAVAPRASAD, C. B.; Dr. MADHAVAPRASAD, C. B.
    The quality and safety of raw milk produced from farm animals assumes utmost importance. The present study was planned to know the quality and safety aspect of raw milk produced from farm animals. Cross sectional studies were planned with a single time raw milk collection in micro watershed areas of Davanagere district for analysis of various parameters. The results of the present study revealed that TVC were within the BIS specifications (<2 lakhs) for raw milk of bovines where as for YMC, there are no specifications by any regulatory agencies. However YMC counts were in the higher range. The prevalence of milk borne pathogens in raw milk was reported as 38.68 per cent of EPEC, 24.53 per cent of EIEC, 15.09 per cent of ETEC, 15.09 per cent of STEC, 19.57 per cent of enterotoxin producing S. aureus, 70.73 per cent of enterotoxin producing CoNS and 0.008 per cent of L. monocytogenes and presence of these pathogens is not permitted by FSSAI standards (FSSAI, 2012). The antimicrobial resistance profile of the isolates as indicated by the MAR index ranged between 0.06-0.94 for S. aureus with 34 MDR strains out of 46 isolates, 0.08-0.83 for E. coli with 12 MDR strains out of 106 isolates and 0 resistance for L. monocytogenes. The overall prevalence of subclinical mastitis in the present study was found to be 20 per cent. Highest incidence of subclinical mastitis was recorded in HF cross (33.33%) followed by Jersey cross (25%), Non-descript (18.33%) and Buffalo (3.33%). The overall mean concentration of trace minerals like zinc, copper and iron was found to be 2.43±0.07, 0.49±0.02 and 4.4±0.21 ppm respectively that remained within the RDA values and the toxic minerals like lead, arsenic and mercury was found to be 1.72 ±0.06, 6.15±0.44 and 15.37±0.81 ppm respectively that exceeded the maximum permissible levels (FAO, 2012). Keeping the various parameters analysed, the quality of milk produced from farm animals in the study area was found to be inferior in quality and unsafe for consumption purpose as this may affect health of the consumers.
  • ThesisItemOpen Access
    STUDIES ON CERTAIN QUALITY PARAMETERS AND PREVALENCE OF PATHOGENS OF PUBLIC HEALTH SIGNIFICANCE IN RAW MILK OF FARM ANIMALS
    (KARNATAKA VETERINARY, ANIMAL AND FOISHERIE'S SCIENCES UNIVERSITY, BIDAR, 2017-08) TARINI, N. K.; Dr. MADHAVAPRASAD, C. B.
    The quality and safety of raw milk produced from farm animals assumes utmost importance. The present study was planned to know the quality and safety aspect of raw milk produced from farm animals. Cross sectional studies were planned with a single time raw milk collection in micro watershed areas of Davanagere district for analysis of various parameters. The results of the present study revealed that TVC were within the BIS specifications (<2 lakhs) for raw milk of bovines where as for YMC, there are no specifications by any regulatory agencies. However YMC counts were in the higher range. The prevalence of milk borne pathogens in raw milk was reported as 38.68 per cent of EPEC, 24.53 per cent of EIEC, 15.09 per cent of ETEC, 15.09 per cent of STEC, 19.57 per cent of enterotoxin producing S. aureus, 70.73 per cent of enterotoxin producing CoNS and 0.008 per cent of L. monocytogenes and presence of these pathogens is not permitted by FSSAI standards (FSSAI, 2012). The antimicrobial resistance profile of the isolates as indicated by the MAR index ranged between 0.06-0.94 for S. aureus with 34 MDR strains out of 46 isolates, 0.08-0.83 for E. coli with 12 MDR strains out of 106 isolates and 0 resistance for L. monocytogenes. The overall prevalence of subclinical mastitis in the present study was found to be 20 per cent. Highest incidence of subclinical mastitis was recorded in HF cross (33.33%) followed by Jersey cross (25%), Non-descript (18.33%) and Buffalo (3.33%). The overall mean concentration of trace minerals like zinc, copper and iron was found to be 2.43±0.07, 0.49±0.02 and 4.4±0.21 ppm respectively that remained within the RDA values and the toxic minerals like lead, arsenic and mercury was found to be 1.72 ±0.06, 6.15±0.44 and 15.37±0.81 ppm respectively that exceeded the maximum permissible levels (FAO, 2012). Keeping the various parameters analysed, the quality of milk produced from farm animals in the study area was found to be inferior in quality and unsafe for consumption purpose as this may affect health of the consumers.