MOHAMMED ARSHAD
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Date
2019
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Publisher
KVAFSU,BIDAR
Abstract
Zoonotic aspects of mycobacteria transmitted through cattle, wild birds and associated environments underscores a serious public health threat. Infections caused by members of MAC depends on many factors.Infections caused by the zoonotic members of the MAC are often neglected with respect to prevalence and animal and human health risks. A cross sectional study was conducted in Shivamogga region of Karnataka by screening a total of 318 samples (cattle-100, wild birds – 100, associated environments- 118) by the use of five target multiplex PCR and nested multiplex PCR for the detection of MAC and MTBC respectively. When screened for the presence of Mycobacterium species, prevalence of 11 % and 20 % were obtained from cattle and associated environments respectively. Wild birds showed a prevalence of 6.73 % and the associated environments had a prevalence of 18.18 %. Prevalence of MAC in cattle and associated environments were found to be 7 % and 4.28 % respectively. Wild birds had a prevalence of 2.88 % and the associated environments showed a prevalence of 2.27 % for MAC. However, prevalence of Mycobacterium avium subspecies paratuberculosis (Map) in cattle and associated environments was same as that of MAC .None of the samples showed presence of MTBC when detected by a nested multiplex PCR. The study concluded that, mycobacteria are widespread organisms which can be detected by molecular tools like PCR. It also emphasized the need to include non tuberculous mycobacteria while designing the studies related to Mycobacterium spp as they significantly contribute to zoonotic infections.
Keywords: Mycobacterium species, MAC, Cattle, wild birds, five target multiplex PCR, nested multiplex PCR.