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Subject: Veterinary Public Health × Author: MAMATHA S. P. × Start date: 2006 ×
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    ThesisItemOpen Access
    PREVALENCE AND CHARACTERIZATION OF LISTERIA SPECIES AT DIFFERENT ECO-EPIDEMIOLOGICAL UNITS OF ANIMAL INTERFACE
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) MAMATHA S. P.; MADHAVAPRASAD C.B.
    The current study was undertaken with the objective of studying Listeria species from different eco-epidemiological units of animal interface in the rural and peri-urban areas of Shivamogga district. A total of 310 samples were collected, analysed, characterized by biochemical, sugar fermentation and molecular methods. The overall prevalence of Listeria species was found to be 2.5%. Epidemiological unit wise, the prevalence of Listeria species was found to highest in the (Abbalgere) 4.4%, (Massur) 4% followed by (Bhadravathi) 3.63%, (Anupinakatte) 3.63% and (L.F.C, VCS) 1.42%. Culturally, identified Listeria species were subjected to PCR targeting of 16SrRNA, Lmo1030, namA, scrA and Oxidoreductasi genes. Further, the isolates were subjected to PCR targeting the virulence associated genes viz., plcA, hlyA, iap and prfA. Out of 310 samples eight isolates were identified as L.monocytogenes (0.6%), L. ivanovii (0.6%), L. welshimeri (0.6%) and L. grayi (0.6%). Genetic diversity of the isolates were performed by ERIC PCR where six ERIC types/cluster (C1to C6) were found with the Shannon weiver index of 0.752 and Simpson index of 0.928. Antibiogram showed that the eight Listeria species where resistant to bacitracin (62.5%) and cefotaxamie (62.5%), whereas susceptible to ciprofloxacin (62.5%), chloramphenicol (50%) and gentamicin (50%). The MAR index of L. monocytogenes (F113 and M9) was found to be 0.25 and 0.18, L. ivanovii (F82 and VS6) was 0.0625 and 0.125, L. welshimeri ( PF4 and F118) was 0.125 and 0.3125 and L.grayi (S3 and SD1) was 0.0625 and 0.125, respectively. L. monocytogenes and L. ivanovii showed weak biofilm ability at 24hrs were as, L.welshimeri and L.grayi were non biofilm formers. At 48 hrs, L. monocytogenes were moderate biofilm formers and at 72 hrs, L.monocytogenes were strong biofilm formers, L.ivanovii moderate biofilm formers, L.welshimeri and L.grayi were weak biofilm formers KEY WORDS: 16SrRNA, ERIC-PCR, AST, Biofilm