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Subject: Veterinary Physiology × End date: 2022 × Start date: 2022 ×
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    ThesisItemOpen Access
    STUDIES ON METABOLIC AND HEMATOLOGICAL PROFILES OF CROSSBRED COWS WITH LAMINITIS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) DODDABASAVANA GOWDA, K.; A. M. KOTRESH
    Laminitis is a diffuse, aseptic, serous, non-purulent inflammation of the laminar corium of the hoof wall. The present study was undertaken to determine assessment haemato-biochemical profiles in crossbred cows with laminitis reared in the central dry zone of Karnataka. The CB cows considered for the present study were divided into three groups like group-I (N=10) as control group having apparently healthy crossbred cows without laminitis, group- II (N=20) having crossbred cows with acute laminitis, Group- III (N=20) having crossbred cows chronic laminitis. Metabolic profiles such as Blood glucose, NEFA, Total protein, Blood urea nitrogen (BUN), revealed statically significant (P<0.05) increased levels in both laminitis groups compared to control group. In haematological profiles there is a significant decrease (p<0.05) in RBC count, PCV, WBC, and haemoglobin (Hb), however there was no significant difference in other haematological parameters such as MCH, MCV, MCHC, and lymphocyte percentage in laminitis group compared to control. In mineral profile there was a significant decrease(p<0.05) in the calcium noticed however there is no significant difference in phosphorus levels in group II, III, compared to control groups. Correlation of haematological parameters such haemoglobin concentration, RBC counts, and packed cell volume, lymphocytes (%) was made with NEFA which showed negative correlation while with BHBA showed positive correlation except lymphocyte percentage. However, calcium showed positive correlation expect lymphocytes while there was a negative correlation with respect to phosphorus and haematological parameters in laminitis group compared to control. Key words: Laminitis, Metabolic profile, Haematological profile, Hoof disorders.
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    ThesisItemOpen Access
    STUDIES ON BIOCHEMICAL AND HEMATOLOGICAL PARAMETERS IN MALNAD GIDDA COWS UNDER DIFFERENT MANAGEMENT SYSTEMS IN SHIVAMOGGA DISTRICT
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) SUNIL, M.G.; A. M. KOTRESH
    Native breeds of cattle in India are well known for draft purposes, resistance to diseases and adaptability. Shivamogga district of Karnataka is densely populated with Malnad Gidda cows which are small statured cattle and are reared by farmers mostly as subsidiary practice to agriculture in heavy rainfall hilly areas of Western Ghat. System of management in different seasons directly affects health, production and welfare status of an animal. Apparently healthy twenty-four Malnad Gidda cows of same parity were selected and were grouped as, Group- I (N=12), reared under semi intensive system of management, Group- II (N=12), reared under extensive system of management. Blood samples were collected two times in weekly intervals during summer, rainy and winter season and was used for the estimation of biochemical parameters like NEFA, BHBA, Serum Glucose, Total Cholesterol, Total Protein, Albumin, BUN, AST, ALT, Total Bilirubin, Calcium and Phosphorus and haematological parameters TLC, TEC, Hb%, PCV and Total Platelet Count. The obtained values for Malnad Gidda cows in different management practice and during the different seasons, statistically did not vary significantly (P<0.05). Although the sample size was limited, these results might indicate the breed’s natural resistance towards the climatic variations observed in the region of Shivamogga district of Karnataka state as well as the breed’s adaptability to the stress conditions, disease status and the different system of management. Key words: Adaptability, Season, System of management, Malnad Gidda.
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    ThesisItemOpen Access
    STUDIES ON HEMATO-BIOCHEMICAL PROFILE OF MICROSPORIDIAL AND CRYPTOSPORIDIAL INFECTIONS IN DOGS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) AMIT PUTHANIKAR; ANANTHAKRISHNA L. R.
    The Enterocytozoon bieneusi and Encephalitozoon intestinalis are most common microsporidia infects the humans and have also been detected in dogs and other animals causing gastrointestinal infection worldwide. Cryptosporidial infection is a life-threatening in immunosuppressed patient such as HIV infected and organ transplanted individuals. The studies have shown that dogs are intermittent shedders of both microsporidia and Cryptosporidium in the environment and also with a known fact that dogs are the most frequent close companion animals to humans posing a threat of transmission of these pathogens. Hence the present study was undertaken with an objective to investigate occurrence of microsporidia and Cryptosporidium and their hemato-biochemical changes in dogs present around Shimogga district, Karnataka. Upon examination of 124 faecal samples by using species specific PCR it was found to be 4.83% prevalence rate of microsporidia more specifically Enterocytozoon bieneusi. The prevalence rate varied between different places within Shivamogga. From the PCR positive samples, pinkish spores can be appreciated further by MT staining method. No samples were found positive for Cryptosporidium by PCR method. Hematological and biochemical parameters analyzed in microsporidia positive samples revels there was a limited changes in lymphocyte count, ALT, creatinine and total bilirubin concentration. The observations in the current study conclude that, microsporidial infection was prevalent in the dogs of Karnataka. For the first time, the zoonotic species, microsporidia Enterocytozoon bieneusi was recorded in dogs. As per the available published literature, this study is the first of its kind on the prevalence of microsporidia in the dogs in India. Key words: microsporidia, Cryptosporidium, Enterocytozoon bieneusi, Modified Trichrome (MT) stain, India
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    ThesisItemOpen Access
    EFFECT OF ASHWAGANDHA AND CITRUS BIOFLAVONOIDS SUPPLEMENTATION ON HEMATO-BIOCHEMICAL PROFILE AND GROWTH PERFORMANCE IN BROILERS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR- 585 401, 2022) SARITA; SRINIVAS REDDY BELLUR
    An experiment was carried out to assess the effect of Ashwagandha and Citrus Bioflavonoids as feed additives on hemato-biochemical profile, antioxidant status and growth performance in broiler. A total of Eighty (80)-day old broiler chicks (Vencobb- 400) were reared commonly and given similar managemental inputs during acclimatization period of two weeks. On 15th day birds were divided randomly and equally into four groups. Group-I (Control) had basal diet. For Group-II, basal diet was supplemented with Ashwagandha extract @1000mg/kg diet, Group-III with supplemental Citrus Bioflavonoids @1000 mg/kg of diet and Group-IV was supplemented with Ashwagandha extract@500mg/kg + Citrus Bioflavonoids @500 mg/kg diet was fed from 15th day to 35 days of age. Blood samples were collected at weekly intervals till the end of experiment. Hematological parameters and antioxidant status were measured manually as per standard protocol. Also, plasma biochemical parameters were estimated in automatic analyzer and growth performance was measured by calculating weekly feed intake, body weight, weekly body weight gain, FCR and dressing per centage. The results suggested that dietary supplementation of Ashwagandha and Citrus Bioflavonoids alone and in combination in broilers had positive influences on all haematological parameters and rather has shown noticeable improvement in Hb content and lymphoproliferative property in broilers. Besides, it had significantly (P< 0.05) lowered glucose, cholesterol, triglyceride and creatinine levels and positive effects on plasma proteins. Significantly (P< 0.05) lower LPO and higher SOD, CAT and GPx activity were observed in Group-II, III and IV compared to the Group-I. In addition, improved BW, WBWG, FI, FCR, and dressing percentage were noted in supplemented broiler chickens. In conclusion these results were indicative of Ashwagandha and Citrus Bioflavonoids alone and in combination are beneficial to improve plasma-biochemical parameters, antioxidant status and growth performance in broilers.
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    ThesisItemOpen Access
    EFFECT OF DIETARY SUPPLEMENTATION OF QUERCETIN AND LYCOPENE ON HAEMATO-BIOCHEMICAL PROFILE AND GROWTH PERFORMANCE IN BROILERS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) RAVIKANTH; SHRIKANT KULKARNI
    An experiment was carried out to investigate the effects of dietary supplementation of quercetin and lycopene on hematological, biochemical, antioxidant and growth parameters in broilers. Day old commercial broiler chicks (80 Nos) were reared commonly and given similar managemental inputs during acclimatization period of two weeks. On 15th day the broilers were divided randomly and equally into four groups. Group I (Control) provided with basal diet, in Group II basal diet were supplemented with quercetin @ 100 mg/ kg of diet, in Group III basal diet were supplemented with lycopene @ 100 mg/ kg of diet and in Group IV basal diet were supplemented with quercetin @ 50 mg/ kg + lycopene @ 50 mg/ kg of diet. Blood samples were collected at weekly intervals till the end of the experiment. Haematological, biochemical, antioxidant and growth parameters were measured as per standard protocol. The results showed that the supplementation of quercetin and lycopene significantly (P˂ 0.05) increased Hb, PCV, SOD, GSH-Px levels; significantly (P˂ 0.05) decreased Heterophil (%), Heterophil/Lymphocyte (H/L) ratio, MDA, Cholesterol and Creatinine levels during last week of the study. There was significant (P˂ 0.05) increase in TEC, Catalase and Body weight values; significant (P˂ 0.05) decrease in triglycerides and BUN values during last two weeks of the study. There was marked improvement in body weight gain, feed intake, FCR and Dressing percentage. However, treatments showed no significant (P˃ 0.05) difference in the values of MCV, MCH, MCHC, TLC, Lymphocytes, Monocytes, Eosinophils, Basophils, Glucose, Total protein, Albumin, Globulin and A/G ratio during the study period. It is concluded that the supplementation of quercetin, lycopene and their combination in the diet of broilers have positive influence on heamoto-biochemical, antioxidant and growth parameters
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    ThesisItemOpen Access
    EFFECT OF DIETARY BETAINE SUPPLEMENTATION ON PHYSIO-BIOCHEMICAL CHANGES AND MILK YIELD IN DEONI COWS DURING SUMMER
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) INDRALE UTPALA; SHRIKANT KULKARNI
    The present study was conducted to assess effects of dietary betaine supplementation on physio-biochemical profile, anti-oxidant parameters, hormonal profile and milk composition in lactating Deoni cows during summer stress. The study was carried out at LRIC, Deoni farm, Bidar during summer season (April-May) for a period of 8 weeks. Ten lactating cows were randomly divided into control and treatment group with five in each group. Control group was fed basal diet alone. For the treatment group, basal diet was supplemented with anhydrous betaine hydrochloride @ 15 grams per cow per day in the diet during the study period. In respiration and heart rate there were no significant differences noted, rectal temperatures were significantly (P< 0.05) lower in treatment group. TEC, Hb. PCV values revealed significant increase at 4th,6th and 8th weeks of the study. Whereas, Lymphocyte % were significantly higher and neutrophils were significantly lower in treatment group at 2nd,4th ,6th ,8th and 6th ,8th weeks respectively. The bio-chemical parameters viz., plasma glucose and total protein levels were significantly higher (P ≤ 0.05) whereas plasma cholesterol levels were lowered at 8th weeks, triglyceride levels and blood urea nitrogen were significantly (P < 0.05) lower at 6th and 8th weeks respectively. Anti-oxidant profile revealed significant (P <0.05) decrease in plasma lipid peroxidation activity and significant (P< 0.05) increase in the mean plasma SOD, catalase and glutathione peroxidase in treatment group at 6th and 8th weeks respectively. Hormonal profile revealed that the treatment group had significantly (P< 0.05) higher T3 at 8th weeks, T4 at 6th and 8th weeks and lower plasma cortisol levels at 4th,6thand 8th weeks respectively compared to control group. Milk parameters revealed significant (P< 0.05) increase in milk yield at 4th,5th,6th,7th and 8thand milk composition including SNF, milk protein, lactose at 7th and 8th weeks respectively compared to control group during study period. From the results of the present study, it can be concluded that supplementation of betaine is economically beneficial with increased milk yield and composition as well as it was found to ameliorate the ill effects of summers stress through improved physio-biochemical data in our study in betaine supplemented group.