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  • ThesisItemOpen Access
    SERUM AND MILK COMPONENTS IN AMRIT MAHAL, HOLSTEIN FRIESIAN CROSSBRED AND JERSEY CROSSBRED COWS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-09) PRAVEEN, V.; Dr. M. NARAYANA SWAMY
    The present study was undertaken in Amrit Mahal (Group I), Holstein Friesian crossbred (Group II) and Jersey crossbred (Group III) cows at third lactation to assess blood metabolic profile and milk components profile. A total of eighteen cows with six cows in each group were selected randomly from different dairy farms. The blood and milk samples were collected during fourth, eighth and twelfth weeks of lactation. The serum samples were subjected for the estimation of the levels of glucose, total protein, albumin, globulin, A/G ratio, urea nitrogen, total cholesterol, triglycerides, calcium, phosphorus, magnesium, sodium, potassium and chloride. The milk components such as percentage of lactose, fat, proteins and solids-not-fat were measured using ultrasonic lactoscan milk analyser. The pH of milk was measured using digital pH meter. The daily milk yield was also recorded. Serum components such as glucose, total protein and globulin were significantly higher during fourth week compared to twelfth week of lactation in Amrit Mahal cows. The serum lipid and mineral profile were higher in Amrit Mahal cows compared to crossbred cows. The milk profile of different parameters such as lactose, protein, fat and SNF were significantly higher in Amrit Mahal cows compared to crossbred cows. Whereas, milk yield and pH was higher in crossbred cows compared to Amrit Mahal cows. It was concluded that the levels of some of the serum and milk components were higher in Amrit Mahal cows compared to Holstein Friesian crossbred and Jersey crossbred cows. Therefore, Amrit Mahal cows should be conserved for further cross breeding programmes.
  • ThesisItemOpen Access
    SERUM ENZYMATIC AND LIPID PROFILE IN HOLSTEIN FRIESIAN CROSS BRED COWS WITH LOW SOLIDS-NOT-FAT SYNDROME
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2017-09) MANEESHA, H. S.; Dr. M. NARAYANA SWAMY
    The present study was conducted to estimate the enzymatic activities, lipid and blood metabolic profile in Holstein Friesian crossbred cows at 3rd and 5th lactation. Twenty four cows were divided into four groups with each group comprising of six animals based on lactation number and the milk SNF content. Group I, II, III and IV comprised of cows of 3rd lactation with normal milk SNF content, cows of 3rd lactation with low milk SNF content, cows of 5th lactation with normal milk SNF content and cows of 5th lactation with low milk SNF content, respectively. The blood and serum samples were collected from each animal at 4th and 8th week of lactation that were analyzed for blood glucose, blood β-hydroxy butyric acid, serum enzyme activities such as AST, ALT, ALP and LDH, lipid profile components and total serum protein. At 3rd lactation, significantly (P<0.05) higher ALP and HDL-C was noted in normal SNF cows compared to low SNF cows. Significantly lower activity of ALT was found in normal SNF cows at both 4th and 8th week of 3 rd lactation. At 5th lactation, blood glucose was significantly higher in normal SNF cows compared to low SNF cows at both 4th and 8th week of lactation. AST, LDL-C, VLDL-C and BHBA did not vary significantly between groups. LDH was significantly higher at 4 th week and cholesterol was significantly higher at 8 th week of 3rd lactation. Triglycerides and total serum protein were significantly higher in normal SNF cows at 8th week of both the lactations. It was concluded that the decreased blood glucose level in low SNF groups could be a reason for low SNF syndrome in HF crossbred cows.
  • ThesisItemOpen Access
    STUDIES ON HEMATO-BIOCHEMICAL PROFILE AND IMMUNE RESPONSIVENESS TO VACCINATION AGAINST FOOT AND MOUTH DISEASE IN MALNAD GIDDA CATTLE
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2017-07) HEMANTH GOWDA, K.; Dr. M. NARAYANA SWAMY
    The present study was conducted to determine the reference values for hematological parameters, biochemical parameters, female reproductive hormones in Malnad Gidda cattle and the physiogenomic aspect such as genetic polymorphism at MHC DRB3.2 loci and cellular immune responsiveness to FMD vaccination in Malnad Gidda compared to Hallikar X HF crossbred cattle. Significantly lower values were observed in young age groups of both sexes for TEC, hemoglobin, PCV, ESR, TLC, eosinophil per cent, except MCV which was significantly higher in young age groups of both sexes. However, No significant difference was observed for MCH, MCHC, and the percentages of neutrophil, basophil, monocyte and lymphocyte. The levels of different biochemical parameters such as the levels of serum total cholesterol, serum triglycerides, HDL cholesterol, LDL cholesterol and VLDL cholesterol, serum total protein, serum albumin, BUN, creatinine, β-hydroxy butyrate, blood glucose and the activities of AST and ALT did not differ significantly among groups. Significant difference was not observed between winter and summer seasons for any of the hematological and biochemical profile taken up for the study among different groups. The reproductive hormones such as 17β-estradiol and progesterone showed increasing and decreasing pattern respectively, during estrous cycle in Malnad Gidda cattle similar to other breeds of cattle. The two allelic variants MHC DRB3.2*117 and *219 present between Malnad Gidda and Holstein Friesian crossbred cattle could be responsible for better cellular immune responsiveness with significantly higher CD4 lymphocytes population to FMD vaccination in Malnad Gidda cattle compared to Hallikar X HF crossbred cattle.