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Subject: Veterinary Physiology × Author: AMIT PUTHANIKAR × Start date: 2014 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    STUDIES ON HEMATO-BIOCHEMICAL PROFILE OF MICROSPORIDIAL AND CRYPTOSPORIDIAL INFECTIONS IN DOGS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) AMIT PUTHANIKAR; ANANTHAKRISHNA L. R.
    The Enterocytozoon bieneusi and Encephalitozoon intestinalis are most common microsporidia infects the humans and have also been detected in dogs and other animals causing gastrointestinal infection worldwide. Cryptosporidial infection is a life-threatening in immunosuppressed patient such as HIV infected and organ transplanted individuals. The studies have shown that dogs are intermittent shedders of both microsporidia and Cryptosporidium in the environment and also with a known fact that dogs are the most frequent close companion animals to humans posing a threat of transmission of these pathogens. Hence the present study was undertaken with an objective to investigate occurrence of microsporidia and Cryptosporidium and their hemato-biochemical changes in dogs present around Shimogga district, Karnataka. Upon examination of 124 faecal samples by using species specific PCR it was found to be 4.83% prevalence rate of microsporidia more specifically Enterocytozoon bieneusi. The prevalence rate varied between different places within Shivamogga. From the PCR positive samples, pinkish spores can be appreciated further by MT staining method. No samples were found positive for Cryptosporidium by PCR method. Hematological and biochemical parameters analyzed in microsporidia positive samples revels there was a limited changes in lymphocyte count, ALT, creatinine and total bilirubin concentration. The observations in the current study conclude that, microsporidial infection was prevalent in the dogs of Karnataka. For the first time, the zoonotic species, microsporidia Enterocytozoon bieneusi was recorded in dogs. As per the available published literature, this study is the first of its kind on the prevalence of microsporidia in the dogs in India. Key words: microsporidia, Cryptosporidium, Enterocytozoon bieneusi, Modified Trichrome (MT) stain, India