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Subject: Veterinary Pharmacology and Toxicology × Author: MADHU R × Start date: 2020 ×
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    ThesisItemOpen Access
    EVALUATION OF IN-VITRO ANTHELMINTIC ACTIVITY OF CURCUMIN AND PIPERINE NANOPARTICLES
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) MADHU R; PAVITHRA. B.H)
    The present study was carried out with an aim to synthesize and characterize curcumin nanoparticles and piperine nanoparticles and to evaluate the in vitro anthelmintic potential of curcumin- and piperine-nanoparticles against gastrointestinal (GI) nematodes of sheep. Curcumin nanoparticles and piperine nanoparticles were synthesized by using solvent (-) anti-solvent method. Scanning electron microscopy (SEM) analysis showed the average particle size of 32nm and 90nm for curcumin nanoparticles and piperine nanoparticles respectively. Dynamic light scattering (DLS) analysis showed hydrodynamic diameter of 261nm and 228nm for curcumin nanoparticles and piperine nanoparticles respectively. The zeta potential of curcumin nanoparticles and piperine nanoparticles was measured to be -24.9mV and -10.2mV respectively. Polydispersity index of curcumin nanoparticles and piperine nanoparticles was measured to be 25.4 per cent and 24 per cent respectively. Curcumin, piperine, curcumin nanoparticles and piperine nanoparticles were investigated for in vitro anthelmintic potential with albendazole as standard of comparison, against GI nematodes of sheep at various concentrations (0.025, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 μg/mL). The in vitro anthelmintic assays employed in the present study were egg hatch assay (EHA), larval development assay (LDA) and larval motility assay (LMA). The piperine nanoparticles (LC50: 0.035μg/ml) showed the good anthelmintic activity in terms of EHA compared to albendazole (LC50: 0.169μg/ml), showed the good anthelmintic activity in terms of EHA compared to albendazole (LC50: 0.152μg/ml), curcumin (LC50: 0.214μg/ml) and curcumin nanoparticles (LC50: 0.056μg/ml) in the present study. The piperine nanoparticles (LC50: 0.062μg/ml) showed the good anthelmintic activity in terms of LDA compared to albendazole (LC50: 0.142μg/ml), piperine (LC50: 0.169μg/ml), curcumin (LC50: 0.094μg/ml) and curcumin nanoparticles (LC50: 0.071μg/ml) in the present study. The curcumin nanoparticles (LC50: 0.071μg/ml) exhibited good anthelmintic activity in terms of LMA compared to albendazole (LC50: 0.142μg/ml) and curcumin (LC50: 0.094μg/ml), whereas piperine and piperine nanoparticles did not show inhibitory effect on L3 larval motility in the present study. Key words: Curcumin nanoparticles, piperine nanoparticles, in vitro anthelmintic assays.