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Theses (PG)

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2010 - 201921
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Subject: Veterinary Parasitology × End date: 2019 × Start date: 2010 × Type: Thesis ×
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Now showing 1 - 9 of 21
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    ThesisItemOpen Access
    MOLECULAR CHARACTERISATION AND IDENTIFICATION OF IMMUNOREACTIVE POLYPEPTIDES OF FILARID NEMATODES OF DOGS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCIES UNIVERSITY, BIDAR, 2019) ARAVINDA, K.; ANANDA, K. J.
    A study on the molecular characterization and identification of immunoreactive polypeptides of filarid nematodes of dogs in Mangalore, Karnataka state was conducted. Different methods were used for detection of microfilariosis in dogs. A total of 250 dogs screened during the present study, 62 % harboured microfilaria. In the present study, three methods used for detection of microfilariosis in dogs. Modified knott’s method detected 94.19 % of the positive samples, direct wet smear and giemsa’s stained blood smear examination detected 61.93 and 57.42 % positive samples. Morphological studies of microfilaria were done by using giemsa’s and methylene blue phosphate staining techniques revealed microfilariae were unsheathed with blunt head and a tapering tail and cuticle appeared striated. The length and width of microfilaria when measured by giemsa’s and modified knott’s technique were 292.2 ± 1.267 μm, 334.9 ± 4.573 μm, 10.65 ± 0.068 μm and 6.862 ± 0.133 μm respectively. The histochemical staining technique revealed acid phosphatase activity at anal pore region of microfilaria by showing red azo dye indicating the microfilaria were of D. repens. Confirmatory of identification of species was done by PCR using species specific primers showing amplification of mitochondrial cytochrome oxidase subunit 1 (COI) gene with single amplicon 350 bp indicated D. repens. The protein profile of microfilarial and adult worm antigen of D. repens was evaluated using SDS-PAGE. The protein profile of microfilarial antigen showed six polypeptides ranging between 183 kDa to 29 kDa. A total of thirteen polypeptides were identified in adult worm antigen ranging from 208 kDa to 29 kDa. The immunoreactive polypeptides detected on western blot with microfilarial and adult worm antigen of D. repens using known positive serum with anti-dog IgG conjugate at 1:1000 dilution revealed 50 kDa and 29 kDa. Key words: Molecular characterization, Immunoreactive polypeptides, filarid nematodes, dog, Mangalore.
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    ThesisItemOpen Access
    EVALUATION OF LARVAL EXTRACTS OF CHRYSOMYA MAGGOTS FOR ANTIBACTERIAL PROPERTY
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-08) GEETHA, N.; Dr. PLACID E DʼSOUZA
    In the present study, the larval extracts of Chrysomya maggots was tested for antibacterial property. The maggots were collected from wounds and carcasses in and around Bengaluru, India. The maggots from wounds were morphologically identified as C. bezziana and of carcasses were C. megacephala and C. albiceps. For larval extracts, the larvae were collected from the fly colony of C. megacephala which was successfully maintained in the laboratory, whereas larvae of C. bezziana collected from the fresh wounds due to their obligation of oviposition on fresh wounds. About two hundred, third instar larvae of Chrysomya specie
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    ThesisItemOpen Access
    SYSTEMATIC STUDY ON ARTHROPOD INFESTATION IN SHEEP WITH SPECIAL REFERENCE TO TICKS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-08) SUDHA RANI, R.; (Dr. PLACID E. D’SOUZA
    A systematic study on arthropod infestation in sheep in Karnataka based on season, breed, management system, agroclimatic zone and site preference was undertaken in nine organized and ten unorganised farms. The prevalence of ectoparasite infestations was 20.7% and 43.7% in organised and unorganized farms. Among all the ectoparasites, prevalence of ticks was highest followed by lice, mites, fleas, nasal bots and flies. The ticks were found resistant to deltamethrin, cypermethrin and were susceptible to amitraz using LPT and AIT-DD test. Among the two EPNs assessed, H.indica was found to be more virulent than S.abbasi. Among the phytoacaricides, Carica papaya seed extract was found to be more virulent than Ricinus communis leaves against ticks by LPT and AIT. By microscopy the prevalence rate of Theileria spp was 16.4% and 64% in organized and unorganized farms respectively. By PCR 24.7 % were positive for T.luwenshuni and 4.7% for T.ovis in organised farms. Also 72 % were positive for T.luwenshuni, 19 % positive for T.ovis and 4 % positive for B.ovis in unorganised farms. Staining of tick tissues with Geimsa revealed 13.5% and 10% of parasitic infection in organized and unorganized farms respectively. Similarly, MGP stain revealed 11.86% and 15.55% infection in organized and unorganized farms. Using PCR, the prevalence of T.luwenshuni in H.Kutchensis was 36% and of T.ovis in H.a.anatolicum was 4% in organised farms. Prevalence of T.luwenshuni and T.ovis was 77% in H.kutchensis and 47 % in H. a. anatolicum in unorganised farms. H.kutchensis ticks was considered as potential vector in disease transmission of T.luwenshuni and T.ovis in sheep in Karnataka. Key words: Arthropods prevalence, sheep farms, acaricides, phytoacaricides, EPNs, vector potentiality
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    ThesisItemOpen Access
    PREVALENCE AND MOLECULAR DETECTION OF BABESIA SPECIES IN SHEEP AND GOATS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) SHAKUNTALA KAGE; Dr. G. S. MAMATHA
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    ThesisItemOpen Access
    EVALUATION OF LARVAL EXTRACTS OF CHRYSOMYA MAGGOTS FOR ANTIBACTERIAL PROPERTY
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) GEETHA, N.; Dr. PLACID E DʼSOUZA
    In the present study, the larval extracts of Chrysomya maggots was tested for antibacterial property. The maggots were collected from wounds and carcasses in and around Bengaluru, India. The maggots from wounds were morphologically identified as C. bezziana and of carcasses were C. megacephala and C. albiceps. For larval extracts, the larvae were collected from the fly colony of C. megacephala which was successfully maintained in the laboratory, whereas larvae of C. bezziana collected from the fresh wounds due to their obligation of oviposition on fresh wounds. About two hundred, third instar larvae of Chrysomya species were incubated in 2 ml of ultra-pure distilled water for one hour, centrifuged to 10,000X g and supernatant was used as excretions/ secretions (ES). The ES and whole larval extract of C. megacephala and ES of C. bezziana larvae of third instars were tested for antimicrobial property against Staphylococcus aureus (MRSA and MSSA), Escherichia coli, and Pseudomonas aeruginosa by agar well diffusion method, disc diffusion method and Minimum inhibitory concentration. The ES of C. bezziana was not effective. Contrastingly, the ES of C. megacephala showed zone of inhibition at the lowest concentration of 0.23 µg by Agar well diffusion method except for P. aeruginosa but ES was found effective by disc diffusion method against all the three bacteria. The minimum inhibitory concentration was 1.35 mg for S. aureus. Whereas the whole larval homogenate failed to show the antimicrobial property.
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    ThesisItemOpen Access
    STUDIES ON GASTROINTESTINAL PARASITISM IN HORSES
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2015) VALIBASHA, H
    The global equine population has been estimated as 58 million, according to a report compiled by the Food and Agriculture Organization of the United Nations
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    ThesisItemOpen Access
    EPIDEMIOLOGICAL STUDIES AND MOLECULAR CHARACTERIZATION OF THEILERIA SPECIES IN SHEEP AND GOATS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2016) SHRUTHI, R
    India is predominantly an agricultural country with about 70 per cent of its population depending on income from agriculture.
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    ThesisItemOpen Access
    STUDIES ON GASTROINTESTINAL PARASITIC INFECTIONS IN GOATS
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2010) NAZIR AHMAD TOOKHY
    Goat {Capra hircus), a member of the Bovidae family and subfamily Caprinae is one of the oldest domesticated species. For thousands of years goats have been used for their milk, meat, hair and skins over much of the world. The success of goat husbandry depends upon the disease-free status of the herd, for which knowledge of various disease entities affecting them is of paramount importance. India ranks second in goat population in the world but the literature on various aspects of caprine diseases is comparatively meagre (Kumar and Prasad, 1986).
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    ThesisItemOpen Access
    EPIDEMIOLOGICAL STUDIES ON BABESIOSIS IN DOGS IN BENGALURU DISTRICT
    (Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2017) ROOPESH, M. P
    Dog is the first pet animal to be domesticated worldwide about ten to fifteen thousand years ago and considered as the best companion animal of human beings because of its well-known qualities viz., faithfulness