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ThesisItem Open Access EVALUATION OF LARVAL EXTRACTS OF CHRYSOMYA MAGGOTS FOR ANTIBACTERIAL PROPERTY(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-08) GEETHA, N.; Dr. PLACID E DʼSOUZAIn the present study, the larval extracts of Chrysomya maggots was tested for antibacterial property. The maggots were collected from wounds and carcasses in and around Bengaluru, India. The maggots from wounds were morphologically identified as C. bezziana and of carcasses were C. megacephala and C. albiceps. For larval extracts, the larvae were collected from the fly colony of C. megacephala which was successfully maintained in the laboratory, whereas larvae of C. bezziana collected from the fresh wounds due to their obligation of oviposition on fresh wounds. About two hundred, third instar larvae of Chrysomya specieThesisItem Open Access SYSTEMATIC STUDY ON ARTHROPOD INFESTATION IN SHEEP WITH SPECIAL REFERENCE TO TICKS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-08) SUDHA RANI, R.; (Dr. PLACID E. D’SOUZAA systematic study on arthropod infestation in sheep in Karnataka based on season, breed, management system, agroclimatic zone and site preference was undertaken in nine organized and ten unorganised farms. The prevalence of ectoparasite infestations was 20.7% and 43.7% in organised and unorganized farms. Among all the ectoparasites, prevalence of ticks was highest followed by lice, mites, fleas, nasal bots and flies. The ticks were found resistant to deltamethrin, cypermethrin and were susceptible to amitraz using LPT and AIT-DD test. Among the two EPNs assessed, H.indica was found to be more virulent than S.abbasi. Among the phytoacaricides, Carica papaya seed extract was found to be more virulent than Ricinus communis leaves against ticks by LPT and AIT. By microscopy the prevalence rate of Theileria spp was 16.4% and 64% in organized and unorganized farms respectively. By PCR 24.7 % were positive for T.luwenshuni and 4.7% for T.ovis in organised farms. Also 72 % were positive for T.luwenshuni, 19 % positive for T.ovis and 4 % positive for B.ovis in unorganised farms. Staining of tick tissues with Geimsa revealed 13.5% and 10% of parasitic infection in organized and unorganized farms respectively. Similarly, MGP stain revealed 11.86% and 15.55% infection in organized and unorganized farms. Using PCR, the prevalence of T.luwenshuni in H.Kutchensis was 36% and of T.ovis in H.a.anatolicum was 4% in organised farms. Prevalence of T.luwenshuni and T.ovis was 77% in H.kutchensis and 47 % in H. a. anatolicum in unorganised farms. H.kutchensis ticks was considered as potential vector in disease transmission of T.luwenshuni and T.ovis in sheep in Karnataka. Key words: Arthropods prevalence, sheep farms, acaricides, phytoacaricides, EPNs, vector potentialityThesisItem Open Access PREVALENCE AND MOLECULAR DETECTION OF BABESIA SPECIES IN SHEEP AND GOATS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) SHAKUNTALA KAGE; Dr. G. S. MAMATHAThesisItem Open Access EVALUATION OF LARVAL EXTRACTS OF CHRYSOMYA MAGGOTS FOR ANTIBACTERIAL PROPERTY(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) GEETHA, N.; Dr. PLACID E DʼSOUZAIn the present study, the larval extracts of Chrysomya maggots was tested for antibacterial property. The maggots were collected from wounds and carcasses in and around Bengaluru, India. The maggots from wounds were morphologically identified as C. bezziana and of carcasses were C. megacephala and C. albiceps. For larval extracts, the larvae were collected from the fly colony of C. megacephala which was successfully maintained in the laboratory, whereas larvae of C. bezziana collected from the fresh wounds due to their obligation of oviposition on fresh wounds. About two hundred, third instar larvae of Chrysomya species were incubated in 2 ml of ultra-pure distilled water for one hour, centrifuged to 10,000X g and supernatant was used as excretions/ secretions (ES). The ES and whole larval extract of C. megacephala and ES of C. bezziana larvae of third instars were tested for antimicrobial property against Staphylococcus aureus (MRSA and MSSA), Escherichia coli, and Pseudomonas aeruginosa by agar well diffusion method, disc diffusion method and Minimum inhibitory concentration. The ES of C. bezziana was not effective. Contrastingly, the ES of C. megacephala showed zone of inhibition at the lowest concentration of 0.23 µg by Agar well diffusion method except for P. aeruginosa but ES was found effective by disc diffusion method against all the three bacteria. The minimum inhibitory concentration was 1.35 mg for S. aureus. Whereas the whole larval homogenate failed to show the antimicrobial property.ThesisItem Open Access STUDIES ON GASTROINTESTINAL PARASITISM IN HORSES(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2015) VALIBASHA, HThe global equine population has been estimated as 58 million, according to a report compiled by the Food and Agriculture Organization of the United NationsThesisItem Open Access OBSERVATIONS ON RESISTANCE OF COMMON GASTROINTESTINAL NEMATODE PARASITES OF CATTLE TO ANTHELMINTICS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2007) SUDHA RANI, RLivestock in India plays a vital role in socio-economic structure of our country and serves as the backbone of agrarian economy contributing approximately over 32 per cent of the total share from agriculture to National GDP.ThesisItem Open Access EPIDEMIOLOGICAL STUDIES AND MOLECULAR CHARACTERIZATION OF THEILERIA SPECIES IN SHEEP AND GOATS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2016) SHRUTHI, RIndia is predominantly an agricultural country with about 70 per cent of its population depending on income from agriculture.ThesisItem Open Access STUDY ON CRYPTOSPORIDIOSIS IN BOVINES(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2007) H.K.M. REKHACryptosporidiosis is an emerging zoonotic disease of global importance caused by the apicomplexan, protozoan parasite. Cryptosporidium, inhabits the microvilli of epithelial surface of the gastrointestinal and respiratory tracts of a wide variety of vertebrates, including humans, causing significant morbidity and mortality, which now represents the third major cause of diarrhoeal disease world wide (Spano and Crisanti, 2000).ThesisItem Open Access STUDIES ON THE EFFICACY OF SOME COMMONLY USED ACARIdDES AIUHNST A COMMON CATTLE AND DOG TICK SPECIES(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) PRADEEP, BSTicks belong lo Phylum, Arthropoda and make up the largest collection of creatures in the Order Acarina. Ticks are divided into two groups, soft ticks (Argasidae) and hard ticks (Ixodidae). Ticks transmit more kinds of pathogens than any other group of blood feeding arthropods and among arthropods are second only to mosquitoes in their public health importance worldwide. Ticks and the diseases they transmit are widely distributed throughout the world, particularly in tropical and subtropical regions. It has been estimated that 80 percent of the world's cattle population is exposed to tick infestation (KAO, 1984).
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