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20183
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Subject: Veterinary Parasitology × End date: 2018 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    EVALUATION OF LARVAL EXTRACTS OF CHRYSOMYA MAGGOTS FOR ANTIBACTERIAL PROPERTY
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-08) GEETHA, N.; Dr. PLACID E DʼSOUZA
    In the present study, the larval extracts of Chrysomya maggots was tested for antibacterial property. The maggots were collected from wounds and carcasses in and around Bengaluru, India. The maggots from wounds were morphologically identified as C. bezziana and of carcasses were C. megacephala and C. albiceps. For larval extracts, the larvae were collected from the fly colony of C. megacephala which was successfully maintained in the laboratory, whereas larvae of C. bezziana collected from the fresh wounds due to their obligation of oviposition on fresh wounds. About two hundred, third instar larvae of Chrysomya specie
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    ThesisItemOpen Access
    PREVALENCE AND MOLECULAR DETECTION OF BABESIA SPECIES IN SHEEP AND GOATS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) SHAKUNTALA KAGE; Dr. G. S. MAMATHA
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    ThesisItemOpen Access
    EVALUATION OF LARVAL EXTRACTS OF CHRYSOMYA MAGGOTS FOR ANTIBACTERIAL PROPERTY
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) GEETHA, N.; Dr. PLACID E DʼSOUZA
    In the present study, the larval extracts of Chrysomya maggots was tested for antibacterial property. The maggots were collected from wounds and carcasses in and around Bengaluru, India. The maggots from wounds were morphologically identified as C. bezziana and of carcasses were C. megacephala and C. albiceps. For larval extracts, the larvae were collected from the fly colony of C. megacephala which was successfully maintained in the laboratory, whereas larvae of C. bezziana collected from the fresh wounds due to their obligation of oviposition on fresh wounds. About two hundred, third instar larvae of Chrysomya species were incubated in 2 ml of ultra-pure distilled water for one hour, centrifuged to 10,000X g and supernatant was used as excretions/ secretions (ES). The ES and whole larval extract of C. megacephala and ES of C. bezziana larvae of third instars were tested for antimicrobial property against Staphylococcus aureus (MRSA and MSSA), Escherichia coli, and Pseudomonas aeruginosa by agar well diffusion method, disc diffusion method and Minimum inhibitory concentration. The ES of C. bezziana was not effective. Contrastingly, the ES of C. megacephala showed zone of inhibition at the lowest concentration of 0.23 µg by Agar well diffusion method except for P. aeruginosa but ES was found effective by disc diffusion method against all the three bacteria. The minimum inhibitory concentration was 1.35 mg for S. aureus. Whereas the whole larval homogenate failed to show the antimicrobial property.