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ThesisItem Open Access IMMUNE RESPONSE IN SHEEP TO IMMUNIZATION WITH DNA ENCODING THE VARIABLE REGION OF ANTI-IDIOTYPIC ANTIBODY SPECIFIC FOR HN PROTEIN OF PESTE DES PETITS RUMINANTS VIRUS(KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2010-07) APSANA, R.; SHRIKRISHNA ISLOOR; M.S. SHAILA; D. RATHNAMMA; B. M. VEEREGOWDAThe present work was undertaken to evaluate and compare the immune responses in sheep to a recombinant DNA vaccine coding for VH chain region of anti-idiotypic antibody against HN protein of PPR and conventional PPRV vaccine. Twenty four seronegative sheep to PPRV antibodies were subjected to vaccination trials. Recombinant DNA vaccine and conventional PPRV vaccine were administered on days 0 and 21. Humoral immune response in vaccinates was evaluated by indirect ELISA and Serum Neutralization Test (SNT). The present study revealed high antibody titres in conventional cell culture PPRV vaccinated groups than recombinant DNA vaccinated group. However, the serum samples were having neutralizing titer of ≥ 8 in both the groups. Indirect ELISA results showed significant difference between the recombinant DNA vaccinated group and control groups indicating the capability of recombinant DNA vaccine to elicit immune response in the total absence of antigen. The preliminary study carried out infer that recombinant vaccine against PPR can be successfully used for protecting sheep against PPR and further overcomes the handling of PPRV for production of conventional cell culture vaccine.ThesisItem Open Access MOLECULAR CHARACTERIZATION OF E.coli FROM BOVINE MASTITIS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2007) VIVEKA PRABHU, BMastitis is an important problem in dairy farms and Escherichia coli is a pathogen of world wide importance, with reference to mastitisThesisItem Open Access COMPARATIVE EVALUATION OE RT-PCR EOR VP3, VP7 AND NS3 GENES IN DETECTING BLUETONGUE VIRUS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) VINAY, SThe Indian farming community mostly comprises of farmers of poor section having sheep and goat as their integral part contributing significantly to the nationâ s economyThesisItem Open Access USE OF STAPHYLOCOCCUS PROTEIN-A AS AN IMMUNODIAQNOSTIC AGENT FOR IDENTIFICATION OF STAPHYLOCOCCUS MASTITIS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2005) PRADIP KUMAR DASThe cow is a foster mother of human race. It does more to the human welfare than any other animal. The cows and buffaloes produce milk that has made important contributions to manâ s health and standard of living.ThesisItem Open Access USE OF IMMUNODIAGNOSTIC METHODS FOR DIAGNOSIS OF CANINE PARVOVIRUS INFECTION(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2005) MOHD. MUDASSAK CHANDAParvoviruses are among the smallest, simplest eukaryotic viruses and were only discovered in the 196Oâ s. They are widespread in nature. Canine parvovirus (CPV) is of great concern to veterinarians, pet owners and dog breeders. Canine parvovirus is a DNA virus with ~25nm in diameter, icosahedral symmetry. There are 3 capsid proteins VPl, VP2 and VP3. The canine parvovirus genome is linear, non-segmented, single stranded DNA of 5kb.ThesisItem Open Access EVALUATION OF BACTERIAL CONTAMINATION AND DETECTION OF SPECIFIC FOOD BORNE BACTERIAL PATHOGENS BY CULTURAL AND PCR BASED TECHNIQUES IN CHICKEN MEAT(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2007) SURESH BABU. TIndia produces over two million tons of poultry meat which accounts to around three per cent of the world's total meat productionThesisItem Open Access ISOLATION OF BACTERIOPHAGE AND ITS EVALUATION OF THERAPEUTIC EFFICACY ON EXPERIMENTALLY INDUCED ESCHERICHIA COU INFECTION IN CHICKENS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2008) CHArrHRA.B.SAn ever changing path of innovation and modernization has lead to the metamorphosis of primitive poultry farming system into an industry which is highly profitable, contributing substantially to national exchequer and making India one of the top nations in the field of poultry production.ThesisItem Open Access EVALUATION OF BOVINE MASTITIS CAUSING Staphylococcus aureus BIOFILM BASED VACCINE IN RABBITS(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2010) SHOAIB AHMADIn the Indian subcontinent, agriculture is the main occupation of 74 per cent of the rural populationThesisItem Open Access STANDARDIZATION OF INDIRECT ELISA FOR SEROPREVALENCE OF LEPTOSPIROSIS IN BOVINES(Karnataka Veterinary Animal And Fisheries Sciences University, Bidar, 2009) SENTHILNATHAN, GLeptospirosis is a widespread zoonosis caused by Leptospira interrogans, a pathogen capable of infecting most mammalian species through either direct contact with an infected animal or indirect contact with contaminated soil or water.