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Subject: Veterinary Anatomy and Histology × End date: 2022 × Start date: 2020 ×
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    ThesisItemOpen Access
    PREPARATION OF ANATOMICAL MUSEUM SPECIMENS BY USING COMMERCIAL RESIN AND LIQUID SILICONE
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) VENKATESH; GIRISH. M. H
    The objective of present study was to compare different gross staining techniques for brain slices, to standardize the sheet plastination using commercially available resins and preparation of corrosion cast. Three brain staining methods were performed, brain slices were pre stained with different stains like Mulligan’s stain, Alston’s stain and Prussian blue stain to differentiate between grey and white matter. Desirable results were obtained in all the three staining techniques. Upon comparison it was found that Alston’s method of staining was more satisfactory than Mulligan’s and Prussian blue staining. The pre-stained brain slices were used for sheet plastination process using Epoxy resin, Polyester Resin and Polyurethane resin. Among these low setting Epoxy resin did not reacted with the stained brain tissues and produced good quality plastinates in which internal structure of grey matter was well differentiated. For corrosion casts Epoxy resin, Polyester resin, Liquid silicon and Polyurethane foam were used for vascular casts and luminal casts of organs. For vascular casts, Polyester resin was good because of its low viscosity and better perfusion in the capillary network. Major drawback of the Polyester resin was its brittleness in the presence of moisture and vascular casts were crumbled upon handling. However Epoxy resin corrosion casts were stronger and durable. For luminal casting of hollow organs Polyurethane foam yielded better results specially to reproduce air sacs in birds. Resins used in the present study were non-patented, less expensive and easily available. The choice of suitable casting material was determined by a variety of factors, such as viscosity of the resin, setting time or working time and reaction temperature. Key words: Sheet plastination, Corrosion cast, Resin, Brain slice staining.
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    ThesisItemOpen Access
    DEVELOPMENT OF FORMALIN FREE PRESERVATION OF BIOLOGICAL SPECIMENS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2022) BINDUMALA, M. V.; GIRISH, M. H.)
    Glycerine dry mount technique can be an innovative method of preserving biological specimens. In the present study, the specimens like liver, spleen, lung along with trachea, heart, a pair of kidneys, brain and poultry visceral organs were collected from slaughter house and dog limbs were obtained from Department of Veterinary Anatomy, Bengaluru. Dehydration was carried out in three changes of pure acetone for three weeks depending on size of the specimen and impregnation was carried out using glycerine for 3-4 weeks at four different conditions and divided into four groups. The group I organs were dehydrated in normal room temperature and impregnated under forced vacuum. Maximum shrinkage was noticed in brain and minimum in liver. The group II organs were dehydrated in normal room temperature and impregnated under normal atmospheric pressure. Maximum shrinkage was noticed in kidneys and minimum in liver. The group III organs were dehydrated in refrigeration temperature and impregnated under forced vacuum. Maximum shrinkage was seen in poultry viscera (in situ) followed by brain and least by the liver. The group IV organs were dehydrated in refrigeration temperature and impregnated under normal atmospheric pressure. Maximum shrinkage was shown by spleen followed by brain and least by the poultry viscera. The consistency of all organs were soft but there was no change in any morphological details. From economic point of view, the cost of production of each group of specimens was approximately Rs.4000 per group without considering the labour and one-time investment of equipments. The glycerine dry mounted tissue sections were also microscopically evaluated and found compactness and distortion of the cellular structures and poor staining charactertics. Key words: Glycerine dry mount, vacuum, temperature, shrinkage.