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  • ThesisItemOpen Access
    GROSS, HISTOLOGICAL AND HISTOCHEMICAL STUDIES ON THE EYEBALL AND EXTRAOCULAR MUSCLES OF SLOTH BEAR (Melursus ursinus)
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) RAJASHAILESHA, N. M.; Dr. R. V. PRASAD
    The present study was conducted on the gross, histological and histochemical studies on the eyeball, extraocular muscles and bony orbit of sloth bear. The bony orbit was oval in shape with incomplete bony rim and formed by six bones. The shape of the eyeball was almost circular and lens was biconvex in shape. The color of the iris was dark brown and pupil was circular. Choroid showed the presence of semicircular tapetum and its base was placed horizontally ventral to the optic papilla. Extraocular muscles were seven in number, muscle dorsal oblique was longest. The corneal epithelium showed the presence of naked nerve endings and iron pigments in their cytoplasm. Histologically, the iris was made up of stroma, iridial muscles and a posterior epithelium and granula iridica was round and found at the tip of the iris. Tapetum was made up of rectangular shaped cells which were oriented in a brick-like manner. Well distinct Bruch’s membrane was observed. The iron pigments were appreciated in pigmented epithelium of retina and it was devoid of melanin pigments in the area adjacent to tapetal layer of the choroid. In the outer nuclear layer, the nuclei of rods were darkly stained and abundant in number, whereas, nuclei of cones were lightly stained and were few in number. In the inner nuclear layer horizontal cells were located adjacent to outer plexiform layer, bipolar cells were oriented radially with centrally located large heterochromatic nucleus, Muller cells were oriented radially, elongated and located in between bipolar cells. Amacrine cells were located in the innermost aspect of the inner nuclear layer, and capillaries were also observed in the inner nuclear, inner plexiform, ganglionic and nerve fiber layer indicating holangiotic pattern of retina. The nerve fibers converge at the optic disc and emerge as optic nerve and optic papilla was goblet shaped. Extraocular muscles were skeletal in nature showing fast and slow twitch based on the granular and homogenous muscle fibers histologically. Presence of intense PAS positive character indicated EOMs are highly metabolically active and participate in aerobic metabolism.
  • ThesisItemOpen Access
    HISTOLOGY, HISTOCHEMISTRY OF RESPIRATORY SYSTEM AND ULTRASTRUCTURAL STUDIES OF LINING EPITHELIA IN PIG (Sus scrofa domesticus)
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) MANJUNATHA, K.; Dr. K.V. JAMUNA
    The present study was carried out in Department of Veterinary Anatomy, Veterinary College, Hebbal, Bengaluru-24. Respiratory system of pig was broadly classified into conducting and alveolar divisions. The conducting part included nostrils, nasal cavity, nasopharynx, larynx, trachea and different divisions of bronchi upto terminal bronchi.Conducting part was lined by typical respiratory mucosa consisting of pseudostratified ciliated columnar epithelium consisting of ciliated columnar cells, basal cells sustentacular cells and goblet cells except larynx which was lined by stratified squamous non keratinized epithelium. Typical transitional type of epithelium was found lining the vocal folds. Specialized type of olfactory epithelium was found lining the ethmoturbinates. The alveolar or respiratory part includes respiratory bronchiole, alveolar duct, alveolar sac and alveoli. These portions were lined by cuboidal to low columnar cells. Clara cells were found lining the bronchioles. Alveoli were lined predominantly by pneumocytes I which were squamous type and few large cuboidal cells called pneumocytes II. In addition to these alveolar macrophages were also found.Goblet cells lining the respiratory tract and glands within the propria-submucosa were positive for both acid and neutral mucopolysaccharides with variable percentage. No glands or goblets were found in alveolar portion. SEM of conducting portion revealed the ciliated cells, microvillous cells and goblet cells which were part of mucociliary apparatus playing a major role in conditioning of inspired air. SEM of alveolar portion revealed pneumocytes type I and II, macrophages and more importantly thicker connective tissue component within the lung