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20191
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Subject: Fisheries × Author: SHAIK THAHUR AHAMED × End date: 2020 ×
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    ThesisItemOpen Access
    STUDY OF COMBINED EFFICACY OF SYNTHESIZED DOUBLE STRANDED RNA (dsRNA) SPECIFIC TO STRUCTURAL AND NON-STRUCTURAL GENES OF WSSV IN PROTECTING LITOPENAEUS VANNAMEI FROM WSSV INFECTION
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2019) SHAIK THAHUR AHAMED; M.N.VENUGOPAL
    RNA interference (RNAi) is a potential strategy to control shrimp viral diseases, including the white spot disease (WSD) caused by White Spot Syndrome Virus (WSSV). Although a significant progress has been achieved on dsRNA mediated anti-virus strategy development, there is still no treatment available to control spread of the disease. This investigation has mainly focused on utilizing viral structural and non-structural proteins subunit vaccines to protect the host. In the present study the protective and therapeutic efficacy of double-stranded RNAs (dsRNAs) targeting structural (VP281) and nonstructural gene (RR2) of WSSV singly and in combination in controlling viral multiplication in Litopenaeus vannamei was investigated by intramuscular injection. The suppression effects of shrimp mortality by either combined dsRNAs of RR2 and VP281 or dsRNA-RR2 & VP281 alone was monitored for 15 days after viral challenge in both protective and therapeutic experiments. Targeting the combination of RR2 and VP281 genes provided better protection (90% survival) compared to RR2 (80%) and VP281 (70%) in protective experiment indicating that targeting a combination of RR2 and VP281 would be effective in limiting WSSV multiplication. In therapeutic experiment, 80% of shrimp survivals were detected from combined dsRNAs treatment, while RR2, VP281 showed survival rates of 70% and 60 % respectively, suggesting that the combination of dsRNAs can therapeutically clear existing WSSV in shrimp after a relatively short time of injecting.