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20212
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Subject: Aquatic Animal Health Management × End date: 2021 × Start date: 2020 ×
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    ThesisItemOpen Access
    EVALUATION OF DIFFERENT AGENTS TO CONTROL TURBIDITY IN WATER
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2021) THIPPESWAMY, T. S; SHIVAKUMAR M
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    ThesisItemOpen Access
    ISOLATION OF BIOACTIVE MOLECULES FROM NEMATOCYSTS OF JELLYFISH ALONG KARNATAKA COAST
    (KARNATAKA VETERINARY, ANIMAL & FISHERIES SCIENCES UNIVERSITY, BIDAR, 2021) HARSHITHA, H.,; SURESH T
    Marine toxins have received global consideration for their participation in human intoxication. Many marine phyla are well adapted to produce venoms or toxins to protect themselves from associated microfauna, predators, and pathogens. Despite the toxicity, some biotoxins stand as potential drug sources in human and veterinary medicine. Among all marine fauna, Cnidarians are well renowned for producing bioactive peptides used in drug development, as they harbor various biological activities such as anticancer, anti-parasitic anti-inflammatory, radical scavenging, immunomodulatory activities, and many more. In the present study, standardization of the crude venom extract from the jellyfish Acromitus flagellatus was fulfilled by employing the TCA/ Acetone extraction protocol followed by nematocyst disruption using glass beads and PBS (pH 7.4). Bioassay was conducted for the crude venom extract having the protein concentration (0.068 μg/μl) and molecular mass between 66 kDa and 97.4 kDa. The crude venom extract isolated from A. flagellatus showed antimicrobial activity against both the Gram-positive and Gram-negative bacteria. Salmonella enterica serovar Weltevreden showed the highest clear zone (9 mm), and Vibrio alginolyticus displayed the least clear zone (2 mm). However, the crude venom extract lacked haemolytic activity against human and fish erythrocytes. Recently, development of multiple drug resistance (MDR) in pathogenic bacteria is a significant concern in medical science. Isolation of such antimicrobial compounds is very much essential to combat raising concerns on MDR. However, further studies are required to purify and characterize the crude venom extract from the present study to recognize its unique chemical and antimicrobial properties.