Loading...
Theses
Browse
92 results
Search Results
ThesisItem Open Access Induction of mutation in Trichoderma strains for enhancing biocontrol potential and compatibility with fungicides(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Dwivedi, Meenakshi; Vishunavat, K.Biocontrol serves as an effective alternate approach for plant disease management under changing food habits and commercialization of agriculture. Trichoderma has become one of the most successful fungal biocontrol agents (BCAs) in past few decades. The progress in achieving commercial, large-scale usage of biological pest control has been rather slow, major roadblocks being instability under diverse environmental conditions, questionable shelf-life and inconsistent performance, and limited compatibility with many fungicides commonly used in agriculture. BCAs need to be tolerant to chemicals which would be a prerequisite for their application in an integrated pest management (IPM) strategy and could be produced through induction of chemical and physical mutation in popularly used biocontrol species of Trichoderma. The present study was attempted to induce mutation by chemical (ethylmethane sulphonate, EMS) and physical (UV rays and _ rays) mutagens in two Trichoderma strains viz. T. asperellum (Th 14) and T. harzianum (Th 3), to develop mutants tolerant to the fungicides (thiophanate methyl and propiconazole) incompatible with the parent strains, that also possessed comparatively enhanced biocontrol potential. Mutants were developed to tolerate thiophanate methyl (up to 200μg ml-1) and propiconazole (up to 1000 μg ml-1). Mutants Th 3 M1 and Th M6 were found comparatively superior in rhizosphere competence in three crops viz. urd bean, mung bean and chilli. Upon evaluation of growth promoting potential in urd bean, mung bean and chilli, mutants Th 3 M1 (8067, 8600 and 11166 PVI), Th 3 M2 (7233, 7633 and 100500 PVI) and Th 3 M6 (7500, 8033 and 9966 PVI) were found significantly better. On the basis of production of indole acetic acid (IAA), mutants Th 3 M1 and Th 3 M6 were found significantly superior with 40.44 and 40.41 IU ml-1, respectively. The parent strains and all the mutants exhibited 100 per cent parasitisation of R. solani and M. phaseolina at 7 days after contact (DAC) and 12 DAC, respectively, whereas only mutants mutants Th 3 M1, Th 3 M3 and Th 3 M6 were able to completely parasitize F. oxysporum capsici at 15 DAC. Mutants Th 3 M1 and Th 3 M6 performed exceedingly well at 52°C with cfu count of 2.90 and 2.85 log10 cfu ml-1, respectively, as compared to the parent strains and other mutants. In quantitative assay for cellulase, mutants Th 3 M1 and Th 3 M6 were found superior with 163.00 and 123.00 IU ml-1 cellulase activity, respectively, as compared to the parent strains and other mutants. High cellualse activity of the mutants Th 3 M1 and Th 3 M6 could be correlated with their high rhizosphere competence potential. Quantitative assay revealed that mutants Th 3 M6 was best with 122.00 IU ml-1 chitinase activity, followed by Th 3 M1 (98.00 IU ml- 1) and Th 3 M3 (94.33 IU ml-1) as compared to the parent strain Th 3 (48.66 IU ml-1) and other mutants. Among all these secondary metabolites identified during analysis, the maximum peak area of chromatogram was covered by tributyl phosphate followed by benenamine, 2-methyl and quinoline. Their quantity was elevated in the mutants Th 3 M1 and Th 3 M6 as compared to parent strain Th 3. Hence, the present investigation highlighted that induction of mutation led to enhancement as well as diminution of biocontrol characteristics of Trichoderma strains (Th 14 and Th 3). Through chemical and physical mutagenesis, stable mutants Th 3 M1 and Th 3 M6 were obtained to tolerate high concentrations of fungicide propiconazole (1000 and 500μg ml-1). Mutants Th 3 M1 and Th 3 M6 were also found most promising in their performance in various parameters such as rhizosphere competence, growth promoting potential, mycoparasitic potential, conidial thermo-tolerance (upto 52°C), production of cellulase and chitinase enzymes and production of volatile secondary metabolites, as compared to parent strain Th 3. Mutants Th 3 M1 and Th 3 M6 could prove to be very crucial biocontrol component in integrated disease management strategies.ThesisItem Open Access Study of the mechanisms underlying synergy in triple combination of copper-chitosan-Trichoderma based novel fungicide against late blight disease of potato (Solanum tuberosum L.)(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Singh, Akansha; Kumar, J.A ‘triple combination’ comprising of low dose of fungicide (CuOH), plant defence activator compound (Chitosan) and copper tolerant biocontrol agent (Trichoderma asperellum TCMS 36 (MH593785) wasdeveloped at Biocontrol laboratory, GBPUA&T, Pantnagar and field tested over years. In the present study, the copper tolerance mechanism of Trichoderma TCMS 36 was investigated and was found that Trichoderma TCMS 36 showed Copper tolerance mechanism by modulating fatty acid composition of its membrnane in copper containing environment. Specifically, derivatives of linoleic acid, oleic acid, palmitic acid and phosphate ester, which determine the membrane stability of Trichoderma in copper toxic environment. Simultaneously, in ‘Triple combination’ chitotsan may chelate the copper ions making Cu-Chitosan chelated complex, which was not absorbed/adsorbed by the T.asperellum. Hence, high modulation in membrane fatty acid composition was recorded in dual combination of Trichoderma+copper while in Triple combination as Chitosan interfere copper adsorption by Trichoderma not much modulation in fatty acid composition was required. Four important beneficial secondary metabolites with high concentration were produced in case of ‘Triple combination’ (Diphenyle sulphone 12.36%; 6-Pentyl alpha pyrone 61.12%; Tetracontane 6.01% and Pentacosane 3.36%) while in dual combination of Copper + Trichoderma three metabolites (Diphenyle sulphone 10.39%, 6-Pentyl alpha pyrone 69.96%, and Tetracontane 2.98%) were observed. Resistance inducing marker enzymes (APX, GR, CAT and LOX) and non enzymatic antioxidants (Ascorbate, reduced Glutathione and total Flavanoids) were significantly high in triple combination and BTH as compared to single and dual combination within 24-72 hrs post inoculation. Hypersensitive reaction through ROS (H2O2) production was observed in triple combination and BABA treated leaves while excessive oxidative stress damage was found in control. Meanwhile, PR-2 (β1,3-Glucanase) protein and antimicrobial secondary metabolites including phytoalexins production were also significantly higher in Triple combination (1.433 μmol/mim/mg and 12.58 peak area% ) as compared to control ( 0.723 μmol/mim/mg and 2.5 peak area%). Disease severity (91.66%) and pathogen invasion were maximum in control and minimum in ‘Triple combination’ treated leaves (4.53%) in detached leaf assay at 72 hr. post inoculation. Field trial at Pantnagar (plain) and at village Gaja (hills) revealed that triple combination containing ready to use Chitosan-661-500ppm gave better results (4.8% to 8.33%) that were at par with commercially available standard fungicides Mancozeb (5% to 7%) as compared to control (95% to 98.99%). Resistance inducer BTH and BABA managed the disease severity up to a certain limit (25% to 33.33%) under field condition. Synergestic action of all theses mechanisms made the ‘Triple combination’ more effective than single or dual combination. The results revealed that ‘triple combination’ so designed could be a viable alternative to chemical fungicides for late blight disease management as well as for increasing the tuber yield of potato.ThesisItem Open Access Improving the myceliation process of Lentinula edodes (Berk.) Pegler with organic substrates and supplements(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-07) Varunesh Kumar; Mishra, S.K.Lentinula edodes (Berk.) Pegler is one of the lignicolous mushrooms that produces brown sporocarps. It found naturally on dead wood of hardwood trees from sub-tropical to sub-temperate climatic conditions and grows commercially on artificial logs of sawdust of different hardwood trees throughout the world. However, in some parts of the world, wheat straw is used as an alternate of sawdust. Although, the sawdust as well as wheat straw based substrates are taking long time for spawn run. Long exposure of substrate to spawn run results the mushroom cultivation un-economical and also invites contamination of various moulds. It is important to realise how to reduce the duration of spawn run in the substrates (medium). For this view, the present research work was formulated. The strain Le-17-04 of Lentinula edodes, the substrates wheat straw, sawdust of Eucalyptus spp., Sesbania aculeate, Bombax spp., and Poplus spp. and the supplements wheat bran, soybean meal and curd were initially selected for the study to measure duration of spawn run and weight of fresh mushroom alongwith biological efficiency and morphology of fruiting body. Under in-vitro study, the vegetative growth of Le-17-04 was also evaluated on culture media, modified media of 5% extract of each wheat bran, soybean meal and curd, pH and temperature. It was found that Lentinula edodes Le-17-04 was well grew on PDA medium at 24oC from pH 5.0 and pH 6.0 with maximum 90 mm, 83.33 mm and 90 mm radial growth at 15, 12 and 12 days after inoculation, respectively. Wheat straw while, testing in vitro showed highest 119 cm vertical growth of Le-17-04 than the sawdust of each Eucalyptus spp., Sesbania aculeate, Bombax spp., and Poplus spp. In the next experiment, modified medium with 5% wheat bran extract resulted maximum 90 mm radial growth of mushroom mycelium than the remaining modified media of 5% each curd and soybean meal. In crop room study, fastest spawn run of Le-17-04 was achieved within 26.33 days on the medium constituted with eucalyptus sawdust + curd (5%) while, poplar sawdust + soybean meal (5%) did not show any growth of mushroom mycelium. All wheat straw based treatments coupled with 1) 5% wheat bran; 2) 5% soybean meal; 3) 5% curd; 4) mixture of poplar sawdust + eucalyptus sawdust (1:1) and poplar sawdust + curd (5%) were successfully produced mushrooms. The treatment wheat straw + curd (5%) resulted highest 20.33% biological efficiency against the lowest biological efficiency of 11.33% as recorded in the treatment wheat straw + poplar sawdust + eucalyptus sawdust (1:1:1). It was also found that the stipe length and its diameter of mushroom were inversely proportional among the majority of the treatments studied.ThesisItem Open Access Epidemiology and molecular characterization of soybean genotypes for identification of resistant sources against Rhizoctonia aerial blight(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-06) Kumari Surbhi; Singh, K.P.Soybean (Glycine max L. Merill) is one of the oldest and most important Kharif oilseed crop grown throughout the world in tropical and sub tropical climates. Severe attack of Rhizoctonia aerial blight of Soybean caused by Rhizoctonia solani Kühn is one of the major constraints in successful cultivation of Soybean. The disease produces greyish brown spots on leaves, leaf stalks, stem, pods and foliage affecting as hot spot in Tarai region. The weather variables such as temperature, relative humidity, rainfall, wind speed and sunshine hours greatly influence the development of disease. In order to ascertain the impact of weather parameters on disease development, severity and progression of RAB were recorded during 2016 and 2017 in the field. It was observed that disease initiated in the field in the first week of September and per cent disease severity reached maximum in the third week of October. The disease index of RAB of Soybean for the Kharif season of 2016 ranged from 21.48 to 64.33 per cent and in 2017 the disease index ranged from 21.88 to 75.85 per cent. The pooled data of two years showed that out of 47 genotypes evaluated in different state, none of the variety was found resistant to RAB. However, 19 genotypes were moderately resistant, fifteen susceptible and thirteen genotypes were found to be moderately susceptible to RAB. Out of the 19 moderately resistant genotypes, seven genotypes viz. PS-1556, PS-1546, PS-1518, PS-1569, PS-1572, PK-416 and PS-1583 were from Pantnagar. Three genotypes viz. PS-1572, MACS-1460 and SL-979 exhibited lowest disease index during the two years of study. PS-1572 showed minimum increase in the disease severity and AUDPC during the peak period of disease occurrence while cultivars SL-1028, DS-3101, JS-2098, SL-982, PS-1550, PS-1518, SL-1074, MACS-1460, PS-1583 showed moderate increase. The progress of RAB was recorded during the 1st week of Sept on susceptible cultivars which gradually progressed due to favourable environmental conditions up to the third week of October, but slowly declined afterwards. The per cent disease index of RAB of Soybean declined with increase in temperature (maximum and minimum) and relative humidity during morning. However, there was no influence of rainfall and wind velocity on the severity index of RAB. The data of disease progression of RAB was subjected to multiple regression analysis in relation to the corresponding weather variables (max temperature, morning relative humidity, rainfall and sunshine) for developing prediction equations which depict the association of the weather variables to disease severity. Eleven out of the twelve markers showed clear polymorphism for the Soybean genotypes under study. The SSR markers Satt 119, Sat_076, Satt 433, Satt 281, Satt277, Satt245 and Satt 520 were able to differentiate clearly between the resistant and susceptible genotypes and will be useful in the screening of resistant genotypes against Rhizoctonia aerial blight. 18 genotypes of Soybean were screened out which showed resistance response against RAB both in the markers analysis and during the two consecutive growing seasons during 2016 and 2017. The genotypes of Soybean identified as the resistant sources against RAB in the present study, can be utilized in the breeding programmes for development of resistant varieties against the disease and can be recommended in general for cultivation in the field.ThesisItem Open Access Etiology of Rhizoctonia solani Kühn causing web blight disease of urdbean and its management(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-07) Ramkrishna; Yadav, L.B.Blackgram or urdbean [Vigna mungo (L.) Hepper] is one of the most important pulse crops of the ‘vigna’ group and its grain contains not only protein but also phosphoric acid in high amount. The crop is infested by a number of fungal, bacterial and viral diseases. Among them, web blight disease caused by Rhizoctonia solani Kühn. [Thanatephorus cucumeris (Frank) Donk] is one of the major serious constraints in its growth and production. It is said to be one of the most devastating fungal diseases of urdbean leading to heavy yield losses. The present investigations were undertaken to study symptomatology and progressive development of disease, study the effect of different culture media, temperatures and pH on mycelial growth and sclerotia formation and evaluate different fungicides, botanicals and bioagents against the pathogen in vitro and in vivo to develop a suitable integrated management strategy. Web blight symptoms on urdbean plants appeared on roots, stems, petioles as well as pods but the disease was most devastating on foliage. On the infected leaves, mycelium appeared as spider web, the characteristic symptom thus suggesting the name of the disease as "web blight". In vitro, on the basis of morphological and cultural characteristics and pathogenicity test, the pathogen was proved as Rhizoctonia solani Kühn. Out of five different culture media studied, PDA was found best for mycelial growth and development as well as sclerotia formation of Rhizoctonia solani. Best temperature and pH for the growth and development of R.solani was found at 30°C and pH 7, with maximum mycelial growth and sclerotia production. Among the fungicides tested in vitro Fluxapyroxad 62.5/l + Epoxiconazole 62.5/l was most effective against Rsolani and showed 100.00 per cent inhibition of its mycelial growth at 20 ppm. Among the botanicals tested in vitro, Tulsi @ 35 per cent was most effective in mycelia growth inhibition of R.solani i.e. 74.81 per cent in dual culture experiment, biocontrol agent Trichoderma harzianum (Th-14) showed better performance over Pseudomonas fluorescens (Psf 173) in inhibiting mycelial growth of Rsolani i.e. 60.70 per cent Under field conditions,three foliar sprays of fungicide a combination of (Fluxapyroxad 62.5/l + Epoxiconazole 62.5/l) @ 0.2 per cent at 15 days interval and seed treatment with T. harzianum + Carbendazim(4 g+2 g/kg), showed highest control in disease severity i.e. 67.96%, over the cheak .with highest grain yield i.e. 1391.80 Kg/ha followed by seed treatment with T.harzianum + Thiram(4 g+ 3g/kg) i.e 67.26 % with 1355.76 Kg/ha grain yield.ThesisItem Open Access Evaluation of popular cultivars and introgression lines against red rot disease of sugarcane(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-06) Jagoori, Prarthana; Sahu, R.K.Sugarcane (Saccharum officinarum L.) is one of the valuable commercial crops of India and grown chiefly for sugar. It produced more than 75 percent of the sugar and provides raw material for various industries. It grew well in tropical and subtropical and thrives well in various agri-ecological situations. It also produces various economically important by-products such as bagasse, fly ash, pressmud, and molasses etc. As it is a long duration and sugar-rich crop, therefore, suffers from a number of diseases. Majority of them are of fungal origin. Among them, the red rot of sugarcane caused by Colletotrichum falcatum Went. is an important disease which reduces production and productivity of cane at national and international level. In the present study, experiments were conducted to assess the physiological characteristics, in vitro efficacy of botanicals and fungicides, the effect of the crude toxin on leaves, in vivo evaluation of introgression lines (C1), and the status of resistance declination in popular sugarcane cultivars against red rot. According to physiological studies, results clearly indicate that 250C temperature and 7.0 pH values are optimum for the growth of test pathogen. On the basis of cultural characterstics, it was concluded that isolated pathogen from CoS8436 (Pant isolate) resembled with Cf08 and thus no new isolate reported. Seven botanicals viz. Parthenium hysterophorus, Argemone mexicana, Lantana camara, Cannabis sativa and Ageratum conyzoides tested against the test pathogen at 5, 10, and 20 per cent concentrations. Parthenium was found most effective. In vitro efficacy of four fungicides viz. Folicur (Tebuconazole), Bavistin (Carbendazim), Custodia (Azoxystrobin + Tebuconazole), Cabri Top (Metiram + Pyraclostrobin) was evaluated. Carbendazim, Azoxystrobin + Tebuconazole, and Tebuconazole (5, 10, 15, 20 and 50μgml-1) found efficacious (100 percent inhibition) to control the pathogen. Among one hundred and eighty-eight introgression (188) lines (C1), 6 lines namely, CoPant96219GC-1, CoPant96219GC-3, Bo91×Co453-4, Co91269×Co62198-2, CoS91269×Co62198-3, and CoS84×Co89003-11 were found resistant to red rot. Among twelve cultivars of sugarcane, four cultivars viz. CoJ64, CoS8436, Co1148, and CoS767 showed resistance declination and showed susceptible reaction, therefore are prone to C. falcatum Race Cf08 and Cf09. Screening for resistance to red rot disease by using crude toxin of the test pathogen on sugarcane leaves showed that only plants susceptible to fungus were affected by the toxin.ThesisItem Open Access Status of loose smut, karnal bunt, ear cockle diseases and seed quality of wheat in Kumaon and Garhwal regions of Uttarakhand(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-07) Negi, Pradeep; Bijendra KumarWheat (Triticum aestivum L. em Thell.) is grown in almost all the temperate and sub-tropical regions of Uttarakhand. Uttarakhand produces over 8.78 lakh metric tonnes under 3.79 lakh hectares of land with productivity of 2.31 tonnes/ha. Its production is being constrained by several biotic and abiotic factors. However, 3 diseases namely; loose smut, Karnal bunt and ear cockle are objectionable under seed production of wheat in India. These are considered as the main constraints in wheat cultivation as they not only affects the crop yield but grain quality as well. Therefore, the present investigation was carried out to determine status and distribution of loose smut, Karnal bunt and ear cockle diseases and to assess the seed quality of wheat in Kumaon and Garhwal regions of Uttarakhand. Results of the present study revealed that out of 86 samples, only 3 samples from hills of Kumaon and Garhwal were infected with loose smut. However, its incidence was within the permissible limit as prescribed by Government of India. Of the 86 samples, 55 were found to be infected with Karnal bunt. However, only in 11 samples the incidence of Karnal bunt was above the permissible limit. None of the sample showed the presence of nematode galls or cockles. Amongst all, 33 samples could qualify the germination standard (85%) prescribed by the government of India. In Kumaon and Garhwal hills, maximum germination (86.33%), root length (15.30cm), shoot length (11.20cm), seedling vigour (2287.75) and minimum seedling abnormality (7.67%) and rotten seeds (6.00%) were recorded in seed samples of UP 2572. In Bhabar region, maximum germination (89.00%), root length (16.74cm), shoot length (12.05cm), seedling vigour (2562.31) and minimum seedling abnormality (6.00%) and rotten seeds (5.00%) were recorded in the sample of variety PBW 154. However, in the plains of Kumaon and Garhwal, maximum germination (92.0%), root length (15.40cm), shoot length (11.80cm), seedling vigour (2502.40) and minimum abnormal seedlings (4.67%) and rotten seeds (3.33%) were recorded in the sample of variety PBW 502. Overall study indicated that loose smut was confined to hills only. The incidence of Karnal bunt in hill and Bhabar region was more as compared to plains of Uttarakhand. None of the sample showed presence of nematode galls or cockles. The seed samples obtained from hilly areas of Uttarakhand had poor seed quality as compared to seed of plain region. Because of limited approach in hilly areas the availability of quality seeds is very poor hence; farmers are forced to use their own saved seeds. On the other hand in plains of Uttarakhand quality was found to be better, may be because of the presence of several government organizations such as G.B.P.U.A&T, Pantnagar, US&TDC and hundreds of private seed companies which are involved in production and promotion of quality seeds.ThesisItem Open Access Pathogenicity of root knot nematode (Meloidogyne enterolobii Yang & Eisenback) in tomato (Lycopersicon esculentum Mill.) and its management through botanicals(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-08) Rawat, Neha Surendra Singh; Satya KumarThe Tomato (Lycopersicon esculentum Mill.), belongs to the family Solanaceae. It is one of the important crop widely cultivated throughout Asia for its edible fruits which are known to be rich in nutrients. Several biotic and abiotic factors have been reported to be associated with low production of tomato. Among the phytoparasitic nematodes, root knot nematode (Meloidogyne spp) is one of the important plant parasitic nematode known to be important limiting factor in the production and productivity of tomato. Root knot nematode attack tomato plant worldwide and reduce the yield of the crop. Periodic surveys were conducted in Mango and Guava orchard in Horticulture Research Centre, G.B.P.U.A.T, Pantnagar, Meloidogyne spp. were found to be the most prevalent in soil Meloidogyne spp. was the most prevalent nematode with 1.21-39.96% and population density ranged between 89-9289 nematodes per 200cc soil. On the other hand, Criconemoides spp, Hoplolaimus spp, Xiphinema spp and Trichodorus spp exhibited low population densities in soil.Among free living nematodes Dorylaimus spp were quite common in all soil samples with 3.06-27.35 per cent and population density range between 311-1689 nematodes per 200cc soil. These observations demonstrated that different genera of plant parasitic and free living nematodes are wide spread in Mango and Guava orchard in Horticulture Research Centre, G.B.P.U.A.T, Pantnagar. Results on pathogenicity experiment conducted in pot showed that an inoculums level of 500 J2 per kg soil and above significantly reduced the various plant growth parameters (shoot length, root length, fresh shoot weight, fresh root weight, dry shoot weight, dry root weight, number of gall formation and final population of nematodes) over uninoculated control and the maximum reduction was obtained at the inoculums level of 8000 larvae of nematode per kg soil. Increase in the inoculum levels i.e. 100, 250, 500, 1000, 2000, 4000, and 8000 larvae of nematode per kg soil decrease the growth parameter of tomato, respectively. Experiment on egg hatching and mobility of M. enterolobii indicate that Azadirachta indica leaf extract is the most effective and Eucalyptus globulus leaf extract is least among used botanicals. Azadirachta indica leaf extract @ 2.5 per cent concentration at the end of 72 hours exposure was most effective. Egg hatching of the M. enterolobii was directly proportionate to exposure period and inversely proportionate to the concentration of botanical extracts. Whereas, the immobility of the M. enterolobii was directly proportionate to exposure period and concentration of botanical extracts as it increases with increase in concentration. The study on management of M. enterolobii infecting tomato by botanicals indicated that all treatments increased the plant growth over untreated inoculated control, while Azadirachta indica leaf @ 5g per kg soil was the best in increasing the overall plant growth as well as in reducing the galling of M. enterolobii. This was followed by Tagetes erecta, Jatropha curcas, Lantana camara, Calotropis procera, and Eucalyptus globulus.ThesisItem Open Access Studies on growth inhibition of Exserohilum turcicum (Pass) Leonard and Suggs, the causal organism of Northern leaf blight of maize(G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2018-06) Bhatt, Bhagyashree; Pradeep KumarMaize is one of the most important cereal crops of the world and contributes to food security in most of the developing countries. Northern leaf blight disease of maize caused by Exserohilum turcicum (Telomorph: Setosphaeria turcica) is one of the most important fungal diseases of maize. The disease is prevalent in almost all the maize growing areas and cause severe yield loses. Keeping in view the importance of the disease, in vitro and glasshouse studies were conducted to elucidate different aspects of pathogen biology and pathogenic growth inhibiting potential of various compounds. Pathogen produced white cottony mycelia growth which later turned black in colour. Conidia were spindle-shaped with 3-8 septation, and had a protruding hilum. Potato Dextrose Agar Media, 300 C temperature and pH 7 were found best for growth of E.turcicum. In vitro evaluation studies revealed that among fungicides tested Propiconazole 25 EC and Tebuconazole 25.9EC were most effective in inhibiting growth of the pathogen. Heena (Lawsonia inermis) among the botanicals, Lemon tulsi (Ocimum africanum) among essential oils and Th isolate of Trichoderma among the other tested isolates were found to be most effective in inhibiting mycelial growth of the pathogen in vitro conditions. Among different tested concentrations of cow urine, 10% concentration showed maximum growth inhibition of pathogen. Under glasshouse condition among three methods of inoculation spraying method gave best results both in Amar and Surya variety of maize. Four fungicides, botanicals, essential oils, Trichoderma isolates and three concentrations of cow urine which proved most effective under lab conditions were tested under glasshouse conditions. Propiconazole 25 EC showed maximum control over the disease and minimum disease incidence (23.33%) and disease severity (17.67%) was observed in case of Amar variety. Reduced disease incidence (27.67%) and disease severity (26.67%) was recorded in case of Surya variety.